A high fat diet increases mitochondrial fatty acid oxidation and uncoupling to decrease efficiency in rat heart

Elevated levels of cardiac mitochondrial uncoupling protein 3 (UCP3) and decreased cardiac efficiency (hydraulic power/oxygen consumption) with abnormal cardiac function occur in obese, diabetic mice. To determine whether cardiac mitochondrial uncoupling occurs in non-genetic obesity, we fed rats a high fat diet (55% kcal from fat) or standard laboratory chow (7% kcal from fat) for 3 weeks, after which we measured cardiac function in vivo using cine MRI, efficiency in isolated working hearts and respiration rates and ADP/O ratios in isolated interfibrillar mitochondria; also, measured were medium chain acyl-CoA dehydrogenase (MCAD) and citrate synthase activities plus uncoupling protein 3 (UCP3), mitochondrial thioesterase 1 (MTE-1), adenine nucleotide translocase (ANT) and ATP synthase protein levels. We found that in vivo cardiac function was the same for all rats, yet oxygen consumption was 19% higher in high fat-fed rat hearts, therefore, efficiency was 21% lower than in controls. We found that mitochondrial fatty acid oxidation rates were 25% higher, and MCAD activity was 23% higher, in hearts from rats fed the high fat diet when compared with controls. Mitochondria from high fat-fed rat hearts had lower ADP/O ratios than controls, indicating increased respiratory uncoupling, which was ameliorated by GDP, a UCP3 inhibitor. Mitochondrial UCP3 and MTE-1 levels were both increased by 20% in high fat-fed rat hearts when compared with controls, with no significant change in ATP synthase or ANT levels, or citrate synthase activity. We conclude that increased cardiac oxygen utilisation, and thereby decreased cardiac efficiency, occurs in non-genetic obesity, which is associated with increased mitochondrial uncoupling due to elevated UCP3 and MTE-1 levels

Adverse effects of extra-articular corticosteroid injections: a systematic review

<p>Abstract</p> <p>Background</p> <p>To estimate the occurrence and type of adverse effects after application of an extra-articular (soft tissue) corticosteroid injection.</p> <p>Methods</p> <p>A systematic review of the literature was made based on a PubMed and Embase search covering the period 1956 to January 2010. Case reports were included, as were prospective and retrospective studies that reported adverse events of corticosteroid injection. All clinical trials which used extra-articular corticosteroid injections were examined. We divided the reported adverse events into major (defined as those needing intervention or not disappearing) and minor ones (transient, not requiring intervention).</p> <p>Results</p> <p>The search yielded 87 relevant studies:44 case reports, 37 prospective studies and 6 retrospective studies. The major adverse events included osteomyelitis and protothecosis; one fatal necrotizing fasciitis; cellulitis and ecchymosis; tendon ruptures; atrophy of the plantar fat was described after injecting a neuroma; and local skin effects appeared as atrophy, hypopigmentation or as skin defect. The minor adverse events effects ranged from skin rash to flushing and disturbed menstrual pattern. Increased pain or steroid flare after injection was reported in 19 studies. After extra-articular injection, the incidence of major adverse events ranged from 0-5.8% and that of minor adverse events from 0-81%. It was not feasible to pool the risk for adverse effects due to heterogeneity of study populations and difference in interventions and variance in reporting.</p> <p>Conclusion</p> <p>In this literature review it was difficult to accurately quantify the incidence of adverse effects after extra-articular corticosteroid injection. The reported adverse events were relatively mild, although one fatal reaction was reported.</p

Infections associated with edible termites and their public health implications in parts of Imo state, Nigeria

Nutritional dependence on termites for proteins could predispose consumers to various infections. This study was carried out to determine the infections associated with winged-reproductive edible termites at Ahiazu Mbaise Area of Imo State, Nigeria. Four hundred and forty five (445) termites were randomly sampled from four sources/categories. Altogether five species were encountered and only four were properly identified. They were Macrotermes bellicosus, M. herrus, M. natatentis and M. subhyalinus. Of the 445 termites examined, 60(13.48%) were positive for cysts/ova of parasites. The infection rate of cysts/ova found, were Ascaris lumbricoides (7.64%), Hookworm (2.69%), Entaemoeba histolytica (1.12%) and Toxocara species (1.79%). Infection rates concerning sources of collection showed no difference (p&gt;0.05). The species of bacteria isolated from termite&#8217;s intestine included Escherichia coli (37.7%), Staphylococcus aureus (15.09%), Klebsiella spp (24.53%) and Streptococcus faecalis (22.64%). Over 50% of inhabitants-cum-consumers were unaware of possible acquisition and transmission of infections by termites. The medical and emerging zoonotic implications are highlighted.Keywords: Infections, Nutritional dependence, termites, Nigeria International Journal of Natural and Applied Sciences, 6(3): 368-372, 201

Assessment of the effectiveness of intervention strategies in the control of endemic dracunculiasis in Ebonyi state, Nigeria

An assessment study of the effectiveness of intervention strategies in the control of endemic dracunculiasis amongst farming communities of Ezza north, Ebonyi State, Nigeria was carried out. A total of 2,226 persons were physically examined during the initial study prior to the application of intervention measures. A total of 426 (19.1%) subjects had either Dracunculus ulcers or emergent worms. About 18.5% and 20.2% males and females were infected respectively. Infection varied among communities with Iyere (47.9%) and Ekka (43.9%) being significantly higher than others (