Establishment of Methylation-Specific PCR for the Mouse p53 Gene

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene

Injury patterns of medial meniscus posterior root tears

INTRODUCTION: Medial meniscus posterior root tear (MMPRT) can occur in middle-aged patients who have a posteromedial painful popping during light activities. MMPRTs are more common in patients with increased age, female gender, sedentary lifestyle, obesity, and varus knee alignment. However, injury mechanisms of minor traumatic MMPRTs are still unclear. We hypothesized that high flexion activities are the major cause of MMPRTs. The aim of this study was to clarify injury patterns of MMPRTs. MATERIALS AND METHODS: One hundred patients were diagnosed having MMPRTs after posteromedial painful popping episodes. Details of posteromedial painful popping episode, situation of injury, and position of injured leg were obtained from the patients by careful interviews. Injury patterns were divided into 8 groups: descending knee motion, walking, squatting, standing up action, falling down, twisting, light exercise, and minor automobile accident. RESULTS: A descending knee motion was the most common cause of MMPRTs (38%) followed by a walking injury pattern (18%) and a squatting action related to high flexion activities of the knee (13%). The other injury patterns were less than 10%. DISCUSSION: Descending knee motions associated with descending stairs, step, and downhill slope are the most common injury pattern of MMPRTs. High flexion activities of the knee are not the greatest cause of MMPRTs. Our results suggest that the descending action with a low knee flexion angle may trigger minor traumatic MMPRTs

Hydronephrosis associated with antiurothelial and antinuclear autoantibodies in BALB/c-Fcgr2b−/−Pdcd1−/− mice

Because most autoimmune diseases are polygenic, analysis of the synergistic involvement of various immune regulators is essential for a complete understanding of the molecular pathology of these diseases. We report the regulation of autoimmune diseases by epistatic effects of two immunoinhibitory receptors, low affinity type IIb Fc receptor for IgG (FcγRIIB) and programmed cell death 1 (PD-1). Approximately one third of the BALB/c-Fcgr2b−/−Pdcd1−/− mice developed autoimmune hydronephrosis, which is not observed in either BALB/c-Fcgr2b−/− or BALB/c-Pdcd1−/− mice. Hydronephrotic mice produced autoantibodies (autoAbs) against urothelial antigens, including uroplakin IIIa, and these antibodies were deposited on the urothelial cells of the urinary bladder. In addition, ∼15% of the BALB/c-Fcgr2b−/−Pdcd1−/− mice produced antinuclear autoAbs. In contrast, the frequency of the autoimmune cardiomyopathy and the production of anti–parietal cell autoAb, which were observed in BALB/c-Pdcd1−/− mice, were not affected by the additional FcγRIIB deficiency. These observations suggest cross talk between two immunoinhibitory receptors, FcγRIIB and PD-1, on the regulation of autoimmune diseases

Successful Emergency Carotid Endarterectomy after Thrombolysis with Intravenous Recombinant Tissue-Type Plasminogen Activator

Acute internal carotid artery (ICA) occlusion may result in severe disability or death. Revascularization by carotid artery stenting after treatment with intravenous (iv) recombinant tissue-type plasminogen activator (rt-PA) has been documented. However, there are few reports on emergency carotid endarterectomy (CEA) within 24 hours after the iv administration of rt-PA. We treated a 58-year-old man with right ICA occlusion with iv rt-PA. Although partial recanalization of the ICA was obtained, severe stenosis at the origin of the ICA persisted and he developed fluctuating neurological deficits. To prevent progressive stroke he underwent CEA 10.5 hours after rt-PA treatment. Thereafter his blood pressure was strictly controlled under sedation. During and after CEA there were no hemorrhagic complications. Our findings suggest that emergency CEA may be an option to address symptomatic severe residual ICA stenosis even after iv rt-PA therapy delivered in the acute stage

The Recognition of The Childminder Choice Student about The Information Appliance Utilization in The Preschool Education

In this study, we clarified how a childminder choice student understood the use of the information appliance for preschool education. Specifically, we performed a cluster analysis based on student responses that we obtained by Silent Dialogue, and analyzed the answers both quantitatively and qualitatively. Based on the results, we considered methods for increasing effectiveness of future practice. In addition, for training subjects such as“preschool education methods,” we piloted some concrete practices to consider the best approaches for teaching. Finally, the results of this study are discussed and future problems are described

トクシマケン ナンブ ノ キュウキュウ イリョウ ノ ゲンジョウ ト アラタナ トリクミ : ノウシンケイ ゲカ ノ タチバ カラ

A regular neurosurgeon was absent in the southern part II health demographic division of medical services where Kaifu-gun of South Tokushima was located after 2008 and was the present conditions that stroke treatment could not perform enough in comparison with prefecture central part. Furthermore, medical care collapse progressed with the obstetrics and gynecology department and pediatrics closedown at one sweep from the same period and, at the prefectural Kaifu Hospital which was this local nucleus hospital, became hard to maintain the emergency care, and increase of closedown of the emergency outpatient department on Saturday and the emergency transportation to the prefecture central part became remarkable, and it was with a big social problem for Tokushima. A medical difference might occur about stroke medical care in central part and the southern part in Tokushima. We performed the epidemiology survey by stroke patient in the southern II demographic division of medical services （popular name “Kaifu project”） as a President of University of Tokushima discretion project for this fact-finding individually in the University of Tokushima neurosurgery classroom. In addition, I worked on the enlightenment for inhabitants in Kaifu-gun. “Local neurosurgical medical treatment part” was established as a college course financially maintained by private donations of Tokushima University Hospital from November 1，2011． Kaifu Hospital neurosurgery medical treatment was enabled daylong, and emergency correspondence came to be in this way possible. The Kaifu Hospital devises the maintenance policy about Tokushima, local inhabitants, a medical association, a local government and the figure which there should be of “the new Kaifu Hospital” for a tsunami now in the plan of the full-scale reconstruction move to the hill in 2015

ノウソッチュウ センモンイ フザイ チイキ ニオケル ノウソッチュウ チリョウ ト ヨゴ ノ ケントウ : トクシマケン ナンブ Ⅱ ホケン イリョウケン ト トクシマ ダイガク ノウソッチュウ センター トノ ヒカク ケントウ

INTRODUCTION: For cranial nerve diseases, particularly stroke, early-stage treatment by stroke specialists can significantly alter patient prognosis. With respect to the treatment of acute cerebral infarction, t‐PA was approved for health insurance coverage in Japan in November２００５, and has greatly influenced medical practice. However, the use of t‐PA necessitates an expertise in stroke treatment, and the drug cannot be used in areas where stroke specialists are absent. Consequently, disparities may be occurring in stroke treatment among different areas. We accordingly aimed to evaluate stroke patients in the south Tokushima Ⅱ medical areas（south Ⅱ medical areas）without stroke specialists and those transferred to the stroke care unit in the Stroke Center of Tokushima University Hospital（Tokushima University Hospital SCU）, and to clarify the presence or absence of disparities in area-based stroke treatment in Tokushima. METHODS: The subjects were１０３stroke patients in the south Tokushima II medical areas without stroke specialists and ３１７ stroke patients in Tokushima University Hospital SCU, who were assessed between October１，２００９and September３０，２０１０. RESULTS: In the areas without stroke specialists, the prognosis of４２％ of the cerebral infarction patients was related to the absence of stroke specialists, and to geographical disadvantages. In４８％ of the cerebral infarction patients in the areas without stroke specialists, the lapse time after the onset of cerebral infarction exceeded３h at their initial examinations. However, this is considered likely to be improved by the implementation of stroke awareness activities. DISCUSSION: Cerebral infarction, which is common in the elderly, is expected to increase in frequency and severity in the future. Therefore, rigorous prevention, awareness activities to spread super-acute stroke treatment using t‐PA, and medical environmental improvement are necessary

The neutrophil-to-lymphocyte ratio as a novel independent prognostic factor for multiple metastatic lung tumors from various sarcomas

Purpose Sarcomas are among the most refractory malignant tumors and often recur as pulmonary metastasis. Although the presence of a high neutrophil-to-lymphocyte ratio (NLR) has been associated with the prognosis of several malignancies, the relationship between the NLR and sarcoma with pulmonary metastasis is unclear. We investigated the impact of the NLR in patients who underwent surgical resection for metastatic lung tumors from various sarcomas. Methods The subjects of this retrospective study were 158 patients with metastatic lung tumors from various sarcomas, who underwent initial pulmonary metastasectomy between 2006 and 2015. We examined the clinicopathological variables, including the NLR and the characteristics of surgical procedures. Survival was estimated by the Kaplan–Meier method and prognostic factors were evaluated by multivariate analysis. Results Multivariate analysis revealed significantly better survival of the group with an NLR  2 years, and 3 or more pulmonary metastasectomies. Conclusion The NLR immediately before the most recent pulmonary metastasectomy is a novel independent prognostic factor, which may be helpful when considering repeated pulmonary metastasectomy

Impact of Hands-on Experience of a Cadaver Dissection on the Professional Identity Formation of Health Sciences Students

[Background] In Japan, some nursing and health science universities that train nurses and/or clinical laboratory technicians have a curriculum in which students observe medical students performing a cadaver dissection. Observing a cadaver dissection is believed to affect the formation of a student’s professional identity. This study aimed to investigate the effects of observing a cadaver dissection on the professional identity of nursing and clinical laboratory science students to find an effective educational support system for developing professional identity. [Methods] Sophomores majoring in nursing science or clinical laboratory science were asked to complete a questionnaire with a professional identity scale before and after hands-on experience of a cadaver dissection performed by medical students. After their hands-on session was complete, they responded to a free-answer question about acquiring a professional identity. [Results] The professional identity score of nursing students significantly decreased after the hands-on experience of the cadaver dissection. No significant change in professional identity score was observed in the clinical laboratory science students. However, the effect size (r) was moderate. [Conclusion] Although professional identity formation fluctuates immediately after the experience of the hands-on experience of a cadaver dissection, the findings do suggest that these hands-on sessions will be effective for developing their professional identity if educational support is provided to help them utilize what they learned through reflection

膠芽腫においてAd-DKK3の抗腫瘍効果はWnt蛋白と受容体の相互作用の阻害によりもたらされる

Field log book of Mike Gagner, labeled WM-7, 4/14/04, WM-7, 06/26/04, and WM-7, 8/20/04 of project 1418.01 PHABSIM site