5 research outputs found
Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray
Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray
Purpose: In the basic research of prostate cancer, prostate cancer cell lines are very valuable tools to
characterize the biology of prostate cancer. Three cell lines including LNCaP, PC-3 and DU-145 are
frequently utilized. Characterization of molecular characteristics of them is very important. In the
present study, we screened gene expression patterns of human prostate cancer cell lines, LNCaP, PC-3 and
DU-145, using cDNA microarray. Methods: Total RNA from LNCaP, PC-3 or DU-145 cells was
reversely transcribed in the presence of Cy3 or Cy5 as a probe. Mixed probes were hybridized to a slide
glass carrying 557 kinds of cancer-related known genes. Signal intensities of Cy-3 and Cy-5 were
separately scanned, and relative signal ratios of Cy3/Cy5 were analyzed. Gene expression levels of
several genes were quantified by real-time PCR. Results: We detected over-expressed or underexpressed
genes in each cell line. Among these genes,farnesyldiphosphate farnesyltransferase 1, amphiregulin,
E-cadherin, urokinase-type plasminogen activator (uPA), and glutathione peroxidase 1 gene
expression levels were quantified using quantitative real-time PCR. The relation of the gene expression
patterns of these genes was consistent with those assessed by real-time PCR. Conclusions: Gene
expression profiles of human prostate cancer cells varied among cell lines. These profiles will provide
useful information on prostate cancer cell lines in the design of experiments, analysis of experimental
data, and so on