The Preparation of Sodium Azide in Liquid Ammonia. II : The Yield of Sodium Azide

When excess of nitrous oxide was reacted with a liquid ammonia solution of sodium the azide formation was large, and when sodium amide is reacted with excess of nitrous oxide, the azide formation was slower, but the sodium utility was greater than that in the former case

A salmon DNA scaffold promotes osteogenesis through activation of sodium-dependent phosphate cotransporters.

We previously reported the promotion of bone regeneration in calvarial defects of both normal and ovariectomy-induced osteoporotic rats, with the use of biodegradable DNA/protamine scaffold. However, the method by which this DNA-containing scaffold promotes bone formation is still not understood. We hypothesize that the salmon DNA, from which this scaffold is derived, has an osteoinductive effect on pre-osteoblasts and osteoblasts. We examined the effects of salmon DNA on osteoblastic differentiation and calcification in MC3T3-E1 cells, mouse osteoblasts, in vitro and bone regeneration in a calvarial defect model of aged mouse in vivo. The salmon DNA fragments (300 bps) upregulated the expression of the osteogenic markers, such as alkaline phosphatase, Runx2, and osterix (Osx) in MC3T3E1 cells compared with incubation with osteogenic induction medium alone. Measurement of phosphate ion concentrations in cultures showed that the DNA scaffold degraded phosphate ions were released to the cell cultures. Interestingly, we found that the inclusion of DNA in osteoblastic cell cultures upregulated the expression of sodium-dependent phosphate (NaPi) cotransporters, SLC20A1 and SLC34A2, in MC3T3-E1 cells in a time dependent manner. Furthermore, the inclusion of DNA in cell cultures increased the transcellular permeability of phosphate. Conversely, the incubation of phosphonoformic acid, an inhibitor of NaPi cotransporters, attenuated the DNA-induced expression and activation of SLC20A1 and SLC34A2 in MC3T3-E1 cells, resulting in suppression of the osteogenic markers. The implantation of a salmon DNA scaffold disk promoted bone regeneration using calvarial defect models in 30-week-old mice. Our results indicate that the phosphate released from salmon DNA upregulated the expression and activation of NaPi cotransporters, resulting in the promotion of bone regeneration.福岡歯科大学2016年

Degradation rate of DNA scaffolds and bone regeneration.

Scaffolds implanted into bone defect sites must achieve optimal biodegradation rates while appropriately filling the void as new bone formation progresses. We recently developed a unique biomaterial consisting of salmon deoxyribose nucleic acid (DNA) and protamine, which can be used as an osteoconductive scaffold for tissue engineering. The aim of the present study was to elucidate how the degradation rate of the scaffold affects bone regeneration. We examined the relationships between the degradation rate of salmon DNA scaffolds and new bone formation using a rat skin flank subcutaneous model and rat calvarial defect model. The degradation rates of the scaffolds were proportional to the durations of pretreatment with ultraviolet (UV) light irradiation. The biodegradation rates of the scaffolds were also dependent on the duration of UV irradiation, as tested a subcutaneous tissue implantation. Scaffolds irradiated with UV light for 0.5 h maintained gradual biodegradation of phosphate compared with scaffolds irradiated for 0 or 3 h. In the calvarial defect model, we found that new bone formation was higher in rats treated with scaffolds irradiated with UV light for 0.5 h compared with those irradiated with UV light for 0 or 3.0 h. The present results suggest that bioengineering of scaffolds for biodegradation is important to regenerate bone. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.福岡歯科大学2017年

SYNCHRONIZATION AND CHAOS IN THE DISCRETE CHEMICAL OSCILLATION SYSTEM(Session I : Cross-Disciplinary Physics, The 1st Tohwa University International Meeting on Statistical Physics Theories, Experiments and Computer Simulations)

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乳癌原発の転移性尿道腫瘍の1例

患者は57歳女性.外陰部出血のため某婦人科を受診し，外尿道口に腫瘤を指摘され， 1980年5月当科を初診した。約1年前に左乳癌にて左乳房切断術の既往があり，その時の病理診断は浸潤性乳頭腺管癌であった。当科に入院後の精査にて肺，肝，脳に一連の転移性腫瘍を認め，また，外尿道口腫瘍生検にて原発巣との組織型の一致を認めたことより乳癌原発の転移性尿道腫瘍と診断した。進行癌の状態であるため，泌尿器科的には膀胱カテーテル留置にとどめ外科に転科したが3カ月後癌性悪液質にて死亡した。遠際臓器よりの転移性尿道腫瘍はきわめて稀で，本症例は本邦文献上では第2例日と考えられる。A 57-year-old woman with a tumor of the external urethral meatus and a previous primary carcinoma of the breast was presented. Diagnosis of metastatic tumor of the urethra was made at excision biopsy and its simultaneous development in a series of generalized metastases. The perusal of Japanese literatures shows that there has been only one case of metastatic urethral tumor reported up to date