Quantitative evaluation of the cheek area after maxillary advancement and rotation by orthognathic surgery in skeletal maxillary retrusion

福岡歯科大学博士（歯学）2024年度doctoral thesi

A study on the fatigue strength of titanium implants with different connection mechanisms

福岡歯科大学博士（歯学）2024年度doctoral thesi

Three-dimensional Analysis of the Shaping Characteristics and Ability of a Novel Ni-Ti Rotary File.

福岡歯科大学博士（歯学）2023年度doctoral thesi

AMPK activation enhances osteoblast differentiation on a titanium disc via autophagy

福岡歯科大学博士（歯学）2023年度doctoral thesi

Creating 3D constructs with cranial neural crest-derived cell lines using a bio-3D printer

福岡歯科大学博士（歯学）2024年度Objectives: The development of bio-three-dimensional (bio-3D) printers has led to significant advances in regenerative medicine. Three-dimensional constructs, including spheroids, are maintained by extracellular matrix proteins secreted by cells so that the cells can be cultured in conditions closer to the physiological environment. This study aimed to create a useful 3D construct as a model of the dentin-pulp complex. Methods: We examined the expression patterns of extracellular matrix proteins and cell proliferation areas in a 3D construct created using O9-1 cells derived from cranial neural crest cells of mice. The 3D construct was created by sticking the spheroid cultures onto a needle array using a bio-3D printer. Results: Cell proliferation areas along with characteristic expression of tenascin C and DMP1 were evaluated. The expression of tenascin C and DMP1 was significantly enhanced in the spheroids compared to that in two-dimensional cultures. Moreover, cell proliferation regions and tenascin C expression were confirmed in the outer layer of spheroids in the embryonic stem cell medium, with insignificant DMP1 expression being observed. Interestingly, in a 3D construct cultured in calcification-induction medium, DMP1 expression was promoted, and DMP1-positive cells existed in the outermost layer without overlapping with tenascin C expression. Conclusions: The extracellular matrix proteins, tenascin C and DMP1, were expressed in a polarized manner in spheroids and 3D constructs, similar to the findings in the dental papilla. Therefore, these 3D constructs show potential as artificial models for studying odontogenesis.doctoral thesi

Sphingosine-1-phosphate receptor 1-mediated odontogenic differentiation of mouse apical papilla-derived stem cells

福岡歯科大学博士（歯学）2023年度doctoral thesi

DNA damage response induced by etoposide enhances osteogenesis in MG63 cells through autophagy activation

福岡歯科大学博士（歯学）2023年度doctoral thesi

口腔粘膜炎に用いられる茵蔯蒿湯によるカエル坐骨神経の複合活動電位の抑制

福岡歯科大学博士（歯学）2023年度doctoral thesi

JNK inhibition enhances cell–cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes

福岡歯科大学博士（歯学）2023年度doctoral thesi

Effects of Lip-closing Force and Anterior Occlusion Relationship on Lip-closing during Articulation

福岡歯科大学博士（歯学）2023年度doctoral thesi