High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved

Biobutanol: the outlook of an academic and industrialist.

Abstract The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures. Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production

Buildings contain chemicals that are toxic, sensitizing and promote colonisation by microbes of health concern

Antimikrobisia biosideja ja muita bioreaktiivisia kemikaaleja sisältyy rakennus tuotteisiin ja käytetään rakennusten ylläpidossa, huollossa, siivouksessa ja (home)saneerauksessa. Käytetyt aineet ovat pääosin pitkävaikutteisia biosideja, jotka sisätiloissa käytettyinä kertyvät muodostaen tilojen käyttäjille pysyvän kemiallisen rasitteen, koska mekanismit (luonnonvalo, sade, tuuli, biohajottavat mikrobit, jotka niitä hävittäisivät, puuttuvat. Selvitimme kokeellisesti ja kirjallisuudesta näiden kemikaalien: 1) antimikrobista tehoa terveyshaitallisiksi tunnettuihin, toksiineja tuottaviin mikrobilajeihin; 2) herkistävyyttä ja muita vaikutuksia ihmisen ja muiden lämminveristen soluihin; 3) tekijöitä, jotka vaikuttavat mikrobien haitta-aine päästöihin ja läpäisevyyteen rakennuksessa