Sporophytic ovule tissues modulate the initiation and progression of apomixis in Hieracium

Apomixis in Hieracium subgenus Pilosella initiates in ovules when sporophytic cells termed aposporous initial (AI) cells enlarge near sexual cells undergoing meiosis. AI cells displace the sexual structures and divide by mitosis to form unreduced embryo sac(s) without meiosis (apomeiosis) that initiate fertilization-independent embryo and endosperm development. In some Hieracium subgenus Pilosella species, these events are controlled by the dominant LOSS OF APOMEIOSIS (LOA) and LOSS OF PARTHENOGENESIS (LOP) loci. In H. praealtum and H. piloselloides, which both contain the same core LOA locus, the timing and frequency of AI cell formation is altered in derived mutants exhibiting abnormal funiculus growth and in transgenic plants expressing rolB which alters cellular sensitivity to auxin. The impact on apomictic and sexual reproduction was examined here when a chimeric RNAse gene was targeted to the funiculus and basal portions of the ovule, and also when polar auxin transport was inhibited during ovule development following N-1-naphthylphthalamic acid (NPA) application. Both treatments led to ovule deformity in the funiculus and distal parts of the ovule and LOA-dependent alterations in the timing, position, and frequency of AI cell formation. In the case of NPA treatment, this correlated with increased expression of DR5:GFP in the ovule, which marks the accumulation of the plant hormone auxin. Our results show that sporophytic information potentiated by funiculus growth and polar auxin transport influences ovule development, the initiation of apomixis, and the progression of embryo sac development in Hieracium. Signals associated with ovule pattern formation and auxin distribution or perception may influence the capacity of sporophytic ovule cells to respond to LOA.Matthew R. Tucker, Takashi Okada, Susan D. Johnson, Fumio Takaiwa, and Anna M. G. Koltuno

Stable isotopic studies on the origin of magnesite deposits in the Setogawa Group, Shizuoka, central Japan(MEMORIAL VOLUME TO THE LATE PROFESSOR TERUHIKO SAMESHIMA)

Massive magnesite deposits in the Setogawa Group in Shizuoka were formed by an extensive carbonation of serpentinite in an ancient accretionary prism. Stable isotopic investigations of the origin of massive magnesite deposits revealed that a homogeneous deep-seated magmatic carbon mixing with marine limestones may be the main source of the carbon. Many calcite and dolomite veins occur in the serpentines, basaltic and pelitic rocks and based upon carbon and oxygen isotopes are supposed to be of similar origin to carbonate veins found in modern serpentine diapirs of the Izu-Bonin and Mariana is-land arc. In contrast, massive magnesite deposits were formed during the subduction of an active volcano and/or an active ridge, with circulation of magmatic carbon providing the carbon source for the serpentine diapirs.publishe

Online Planning for a Material Control System for Liquid Crystal Display Manufacturing

The hyper-modular printer control project at PARC has proven that a tightly integrated model-based planning and control framework can effectively control a complex physical system. Recently, we have successfully applied this framework to another application: planning for the Material Control System (MCS) of Liquid Crystal Display (LCD) manufacturing plant in a joint project between the Embedded Reasoning Area at PARC and the Products Development Center at the IHI Corporation. The model-based planner created at PARC was able to successfully solve a diverse set of test scenarios provided by IHI, including those that were deemed very difficult by the IHI experts. The short projecttime (2 months) proved that model-based planning is a flexible framework that can adapt quickly to novel applications. In this paper, we will introduce this complex domain and describe the adaptation process of the Plantrol online planner. The main contributions are: (1) introducing a successful application of general-purpose planning; (2) outline the timeline-based online temporal planner; and (3) description of a complex warehouse management problem that can serve as an attractive benchmark domain for planning

Comparison of re-revision rate and radiological outcomes between Kerboull-type plate and metal mesh with impaction bone grafting for revision total hip arthroplasty

BackgroundThis study compared the re-revision rate and radiographic outcomes of revision total hip arthroplasty (THA) using a Kerboull-type acetabular reinforcement device (KT plate) with bulk structural allograft and metal mesh with impaction bone grafting (IBG).MethodsNinety-one hips of 81 patients underwent revision THA for American Academy of Orthopedic Surgeons (AAOS) classification type III defects from 2008 to 2018. Of these, seven hips of five patients and 15 hips of 13 patients were excluded due to insufficient follow-up information (= 60 mm, respectively. The current study compared the survival and radiographic parameters of 45 hips of 41 patients using a KT plate (KT group) and 24 hips of 24 patients using a metal mesh with IBG (mesh group).ResultsEleven hips (24.4%) in the KT group and 1 hip (4.2%) in the mesh group exhibited radiological failure. Moreover, 8 hips in the KT group (17.0%) required a re-revision THA, while none of the patients in the mesh group required a re-revision. The survival rate with radiographic failure as the endpoint in the mesh group was significantly higher than that in the KT group (100% vs 86.7% at 1-year and 95.8% vs 80.0% at 5-years, respectively; p = 0.032). On multivariable analysis evaluating factors associated with radiographic failure, there were no significant associations with any radiographic measurement. Of the 11 hips with radiographic failure, 1 (11.1%), 3 (12.5%), and 7 (58.3%) hips were of Kawanabe classification stages 2, 3, and 4, respectively.ConclusionsThe findings of this study suggest that revision THA using KT plates with bulk structure allografts could provide poorer clinical outcomes than revision THA using a metal mesh with IBG. Although revision THA using KT plates with bulk structural allografts could set the true hip center, there is no association between a high hip center and clinical outcomes. The relationship between the position of the KT plate and the host bone might be considered more carefully