False identification of other microorganisms as Staphylococcus aureus in Southern Nigeria

Purpose: Staphylococcus aureus is the causative agent of many infections and the advent MRSA has drawn much attention to it. However, some organisms have been noted to be wrongly identified as S. aureus through phenotypic identifications leading to wrong treatment of infections. This study is therefore undertaken to evaluate the rate of false identification of other organisms as S. aureus in Southern Nigeria.Methods: 507 microorganisms which have been previously identified as S. aureus in 8 States in Southern Nigeria through characteristic morphology on blood agar, Gram staining, growth and fermentation on Mannitol Salt Agar and coagulase formation were collected. All the isolates were identified in this study through sequencing of 16S rRNA and detection of spa gene. The percentages of true and false identities were determined.Results: Of the 507 isolates previously identified as S. aureus, only 54 (11 %) were confirmed as S. aureus while the rest were coagulase negative Staphylococci (85 % misidentification rate), Bacillus sp. (12 % misidentification rate), and Brevibacterium sp. (3 % misidentification rate).Conclusion: A high rate of false positive identification of S. aureus which could lead to the misuse of antibiotics in emergency situation has been identified in this study. The use of standard methods for the identification of S. aureus at all times is highly recommended.Keywords: Coagulase staphylococci differentiation, misidentification of S. aureus, phenotypic identifications, Southern Nigeri

Occurrence of aminoglycoside-modifying enzymes genes (aac(6′)-I and ant(2′′)-I) in clinical isolates of Pseudomonas aeruginosa from Southwest Nigeria.

Background: Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa.Obejectives: We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria.Methods: A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs.Results and conclusion: Two types of AME genes [aac (6′) – I and ant (2′′) – I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6′) – I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6′′) – II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.Keywords: Aminoglycoside-modifying enzymes, antibiotics resistance, Pseudomonas aeruginos

Cadastral Information System for M.I. Wushishi Housing Estate.

This study presents the possibilities for efficient implementation of a Cadastral Information System for M. I. Wushishi Estate in a GIS environment. Logical and Physical models for the Cadastral Information System were built and utilized in the creation of the Cadastral Information System using an Entity relationship model. Keywords: Cadastral, Information System, Estat

A SURVIVABLE DISTRIBUTED DATABASE AGAINST BYZANTINE FAILURE

Distributed Database Systems have been very useful technologies in making a wide range of information available to users across the World. However, there are now growing security concerns, arising from the use of distributed systems, particularly the ones attached to critical systems. More than ever before, data in distributed databases are more susceptible to attacks, failures or accidents owing to advanced knowledge explosions in network and database technologies. The imperfection of the existing security mechanisms coupled with the heightened and growing concerns for intrusion, attack, compromise or even failure owing to Byzantine failure are also contributing factors. The importance of  survivable distributed databases in the face of byzantine failure, to other emerging technologies is the motivation for this research. Furthermore, It has been observed that most of the existing works on distributed database only dwelled on maintaining data integrity and availability in the face of attack. There exist few on availability or survibability of distributed databases owing to internal factors such as internal sabotage or storage defects. In this paper, an architecture for entrenching survivability of Distributed Databases occasioned by Byzantine failures is proposed. The proposed architecture concept is based on re-creating data on failing database server based on a set  threshold value.The proposed architecture is tested and found to be capable of improving probability of survivability in distributed database where it is implemented to  99.6%  from 99.2%.

Resting serum concentration of high-sensitivity C-reactive protein (hs-CRP) in sportsmen and untrained male adults

There is an inverse relationship between regular physical activity and concentration of serum inflammatory markers, with variations in resting CRP in trained and  untrained subjects. The effect of acute and prolonged exercises has been studied on inflammatory markers with dearth of information and controversies on the resting serum values of high sensitivity CRP (hs-CRP). Therefore, this study sought to  identify and compare variations that occur in serum levels of high sensitivity CRP in groups of sportsmen (6) and physically active untrained subjects. Eighty-one healthy male participants made up of 21 untrained (control), 10 footballers, 10 athletes, 10 karates, 10 volleyballers, 10 basketballers, and 10 baseballers  voluntarily participated in the study. Participants rested while in sitting position for about 30 minutes during which blood pressures and heart rates were taken. 5 mls of venous blood was withdrawn from the antecubital vein of the participants  (aseptically) between 7:00 and 10:00 am into lithium heparin bottles following an overnight fast. The supernatant was decanted and centrifuged at 3000 rpm, serum was collected and stored at -20ºC prior to biochemical assay which was done with the use of enzyme linked immunosorbent assay (ELISA) kits for hs-CRP. Differences in the means within the sporting groups were analysed using one-way ANOVA while the difference between the trained sportsmen and untrained young adults was analysed using the independent T-test. Statistical significance was set at p < 0.05. The Mean ±SEM age, weight, height, and BMI of the participants were 22.0±0.8 years, 64.1±2.2 kg, 1.74±0.3 m, and 20.6±0.2 kg/m2 respectively. The resting concentration of hs-CRP (μg/ml) was 1.0±0.2 in the untrained, 2.6±0.7 in footballers, 3.6±2.1 in track athletes, 2.4±0.5 in basketballers, 2.2±0.5 in volleyballers, 2.4±1.3 in baseballers, and 1.7±0.5 in karate respectively. There was no significant difference in the resting hs-CRP amongst the sporting groups, and in the sportsmen and untrained group. Resting serum levels of hs-CRP falls within normal range, and varies insignificantly in groups of sports, and in sportsmen and untrained young male adults. This may suggest that the types of sporting activities or participation in sports has no impact on the resting serum hs-CRP.Keywords: Resting hs-CRP, Sportsmen, Young male adult

A Remote-Sensing Based Assessment of Seasonal Variation in Vegetation Quality and Productivity in Nimbia Forest

Agriculture plays a vital role in the growth and sustainability of developing countries, such as those in sub-saharan Africa. Therefore, there is need to monitor and assess the changes taking place on vegetation quality and productivity. Conventional ground survey method has proved to be highly tedious, laborious and grossly limited especially when large and densely forested regions are concerned. This paper presents a remote sensing based approach as a real time technology to determine the effect and extent of water loss caused by seasons on vegetation quality in Nimbia forest, Kaduna State. ENVI 4.7, ArcGIS 10.0 and Global Mapper Softwares were used and a discrepancy of about 45% in quality of vegetation has been observed between seasons in the region under study. Keywords: Remote Sensing, Normalized Difference Vegetative Index, ENVI, ArcGIS

Eudragit E100 and Polysaccharide Polymer Blends as Matrices for Modified-Release Drug Delivery II: Swelling and Release Studies

Purpose: To compare the effects of two states of polymer/polymer blending (dry and aqueous/lyophilized) of locust bean gum with Eudragit® E100 and sodium carboxymethylcellulose on swelling and drug (levodopa) release from their tablet matrices.Methods: Sodium carboxymethylcellulose (SCMC), Eudragit® (E100) and locust bean (LB) were blended in their dry (as purchased) state or modified by aqueous blending and subsequent lyophilization prior to use as tablet matrices. The tablets were evaluated for swelling and in vitro drug release. Furthermore, in vivo absorption was predicted from the in vitro release data by convolution method.Results: E100 matrices exhibited little or no swelling while the matrices of SCMC and LB and their blends exhibited a degree of swelling > 180 %. Aqueous blending and lyophilization modulated the rate of release from matrices formulated with LB, SCMC and their polymer/polymer blends. Drug release profiles of the lyophilized polymer/polymer blends matrices were dissimilar to those of the dry polymer/polymer blends. Formulations F1aq, F2aq and F3aq exhibited fairly uniform absorption in the first 8 h, indicating the possibility of producing a steady delivery of drug.Conclusion: Polymer blending of LB, SCMC and E100, achieved by aqueous blending and lyophilization, enhances the performance of the matrices thereby exhibiting controlled levodopa release with no burst effect and the tablets retained their three-dimensional network.Keywords: Controlled release, Drug delivery, Eudragit, Locust bean, Levodopa, Matrix, Polymer blend, Sodium carboxymethylcellulos

Eudragit E100 and Polysaccharide Polymer Blends as Matrices for Modified-Release Drug Delivery I: Physicomechanical Properties

Purpose: To compare the effects of two states of polymer/polymer blending (dry and aqueous/lyophilized) on the physicomechanical properties of tablets, containing blends of locust bean gum (LB) with Eudragit® E100 (E100) and sodium carboxymethylcellulose (SCMC) as matrices.Methods: LB, SCMC and E100 were blended in their dry (as purchased) state or modified by aqueous blending and subsequent lyophilization, prior to use as matrices in tablets. The polymer blends were characterized by infra-red spectroscopy (FTIR), flow and compressibility tests, as well as physicomechanical analysis of their tablets.Results: No significant variations were noticeable in the FTIR peaks of the individual polymers in the dry and the aqueous/lyophilized states. Aqueous/lyophilized blending of the polymers resulted in better flow properties. The aqueous/lyophilized matrices were denser with improved mechanical strength and the tablets were harder than those produced from dry blended polymers.Conclusion: Dry blending of LB with E100 and SCMC greatly improved the physicomechanical properties of the tablets. This was further enhanced by aqueous/lyophilized blending.Keywords: Drug delivery, Polymer blend, Eudragit, Locust bean gum, Levodopa, Sodium carboxymethylcellulose, Matrix, Physicomechanical propertie

Bathymetric Survey and Volumetric Analysis for Sustainable Management: Case Study of Suleja Dam, Niger State, Nigeria

Ocean floor and the shape of the shoreline are major influencers of the changes in tidal propagation. Bathymetry; a survey operation aimed at determining the nature of the underwater level and topography of a water body’s bed level plays a cogent role in the determination of both the shape of the shoreline and the ocean floor thereby aiding the monitoring and spatio-temporal modelling of the changes in tidal propagation as much as it ensures the availability of hydro-data which is the foundation of a Maritime Database Management System (MDBMS). This work presents the findings of the bathymetric survey and volumetric analysis of Suleja Dam located in the North central Region of Nigeria. The Differential Global Positioning System receiver (DGPS) (Promark 3), automatic level, echomap 50s in conjunction with the engine boat were used during this research. The data acquired was processed using the GNSS solutions software with the chart plotted in surfer 9 software environment. The surface area of the dam was discovered to be 24.64ha less than the designed area. Also the volume of water was found to be 3.6 x 106m3 in contrast to the design volume of 34.7x106m3. It was also observed that the lowest water depth was 0.56m and the highest water depth was 22.06m, therefore a difference of 5.94m was obtained as against the initial construction depth of 28.00m. The undulating nature of the reservoir bed and the difference in volume of water and surface area covered by the dam are pointers and clear indications of sedimentation and siltation process going on in the dam. Keywords: Bathymetry, sounding, chart, reservoir, MDBM

Using a GIS-Based Network Analysis to Determine Urban Greenspace Accessibility for Different Socio-Economic Groups, Specifically Related to Deprivation in Leicester, UK

Accessibility to greenspace by different socio-economic groups specifically related to deprivation in English city of Leicester was done using Geographical Information System (GIS) network analysis. The result was compared with ANGSt standard which provided the benchmark for accessing greenspace in UK. The result shows that greater percentage of socio-economically deprived group in Leicester lack access to greenspace within 300m from home. For distances of 2000m and 5000m, 70% do have access. This work has explored the use of GIS network analysis to assess the accessibility of greenspace among socio-economically deprived groups which will help those saddled with the responsibility of providing greenspace in carrying out their duties to ensure equity. This analysis can be extended to other groups in Leicester e.g. the handicap, elderly etc to assess how accessible greenspace is to them. Keywords: GreenSpace, GIS Network Analysis, ANGSt Standards, Deprivatio