A physical and chemical stability study of amphotericin B lipid complexes A (ABELCET®) after dilution in dextrose 5%.

International audienceStudy objectives: Abelcet® is an amphotericin B (AmB) lipid complex administrated within 24 hours after dilution in Dextrose 5% (D5). In order to increase the delay before administration we have investigated the chemical and physical stability of Abelcet® diluted in D5 bags at different AmB concentrations and temperatures over a one-week period. Methods: Evolution of the concentration of AmB was measured by High Performance Liquid Chromatography (HPLC) as a function of these incubation conditions in order to check the chemical stability of AmB. Physical stability of Abelcet® has been followed by laser diffraction particle size analyser. UV spectroscopy and Circular Dichroism (CD) spectra were achieved to determine the evolution of AmB aggregation state. Results: It appears that AmB concentrations were stable, that no major change in the aggregation state of AmB in Abelcet® were observed in CD spectra, while particle size distribution have not shown any evolution. Conclusion: It is concluded that Abelcet® after dilution in D5 at concentration between 0.4 to 2 mg/mL is stable and may be stored during one week at 4 and 25°C

Outcome of home parenteral nutrition in 251 children over a 14-y period: report of a single center

Parenteral nutrition (PN) is the main treatment for intestinal failure

Endothelial function and mechanical properties of the common carotid artery in children on parenteral nutrition

Intravenous administration of nutrition mixtures induces endothelial damage and arterial wall remodeling in animal models. To study endothelial function and common carotid artery mechanical properties in children receiving parenteral nutrition, we used noninvasive ultrasonic measurements in 18 children on parenteral nutrition and 18 controls. No difference appeared in the geometry of the common carotid artery (intima media thickness, systolic and diastolic diameters) between the patients on parenteral nutrition and the controls. The incremental elastic modulus was significantly higher in the patients on parenteral nutrition (1.8 +/- 0.4 versus 1.4 +/- 0.5 4 mm Hg x 10(3), p < 0.05) reflecting alteration of the elastic properties of the arterial wall independent of the vessel geometry. The flow-mediated dilatation of the brachial artery was significantly lower in the patients on parenteral nutrition (6 +/- 3 versus 8 +/- 3%, p < 0.05), whereas the dilatation after glyceryl trinitrate administration was similar (22 +/- 9 versus 25 +/- 9%). Children on parenteral nutrition exhibit endothelial dysfunction and altered stiffness of the common carotid artery. The noninvasive methods used in this study may prove useful for objectively determining the effects of various preventive methods