Antimicrobial effects of propolis on Clostridium difficile strains belonging to the different PCR-ribotypes

Clostridium difficile is a Gram-positive, anaerobic rod-shaped bacterium, which is a primary cause of nosocomial diarrhoea. The rising incidence of antibiotic resistance in pathogens such as C. difficile makes the new antibacterial natural products research very important and necessary to conduct. It has been observed that propolis has bactericidal effects. Propolis is a natural resinous product that is manufactured by honeybees (Apis mellifera). The antimicrobial activity of propolis is an important biological property. In spite of the large composition differences of the propolis types, all of them have antimicrobial activity. Twenty strains of the C.difficile belonging to four prominent PCR-ribotypes (RT) (RT017, RT023, RT027, and RT046) were used in research. MBC value were determined by broth dilution method. Propolis samples were obtained from honey bees farm in lubelskie woyevodship. Bactericidal effect of the propolis on C.difficile strains was observed at its concentration of  39 mg/ml

Antimicrobial effects of propolis on Clostridium difficile strains belonging to the different PCR-ribotypes

Clostridium difficile is a Gram-positive, anaerobic rod-shaped bacterium, which is a primary cause of nosocomial diarrhoea. The rising incidence of antibiotic resistance in pathogens such as C. difficile makes the new antibacterial natural products research very important and necessary to conduct. It has been observed that propolis has bactericidal effects. Propolis is a natural resinous product that is manufactured by honeybees (Apis mellifera). The antimicrobial activity of propolis is an important biological property. In spite of the large composition differences of the propolis types, all of them have antimicrobial activity. Twenty strains of the C.difficile belonging to four prominent PCR-ribotypes (RT) (RT017, RT023, RT027, and RT046) were used in research. MBC value were determined by broth dilution method. Propolis samples were obtained from honey bees farm in lubelskie woyevodship. Bactericidal effect of the propolis on C.difficile strains was observed at its concentration of  39 mg/ml

Clonal Spread of a Clostridium difficile Strain with a Complete Set of Toxin A, Toxin B, and Binary Toxin Genes among Polish Patients with Clostridium difficile-Associated Diarrhea

Clinically relevant Clostridium difficile strains usually produce toxins A and B. Some C. difficile strains can produce an additional binary toxin. We report clonality among five strains carrying all toxin genes from Polish patients with C. difficile-associated diarrhea. In another strain, possible recombination between binary toxin genes is documented

Toxin Profiles and Resistances to Macrolides and Newer Fluoroquinolones as Epidemicity Determinants of Clinical Isolates of Clostridium difficile from Warsaw, Poland

Amplified fragment length polymorphism genotypes, antibiotic resistance profiles, and toxin profiles of Clostridium difficile strains from Warsaw were determined. The isolates segregate in six major genotypes, coinciding with toxin profiles. Most of the toxin A-negative toxin B-positive toxin CDT-negative strains possess ermB, and several strains were resistant to fluoroquinolones. Resistograms and toxin types of C. difficile strains are epidemicity determinants