19 research outputs found

    Cross-Species Network Analysis Uncovers Conserved Nitrogen-Regulated Network Modules in Rice

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    In this study, we used a cross-species network approach to uncover nitrogen-regulated network modules conserved across a model and a crop species. By translating gene “network knowledge” from the data-rich model Arabidopsis (Arabidopsis thaliana) to a crop (Oryza sativa), we identified evolutionarily conserved N-regulatory modules as targets for translational studies to improve N-use efficiency in transgenic plants. To uncover such conserved N-regulatory network modules, we first generated a N-regulatory network based solely on rice (O. sativa) transcriptome and gene interaction data. Next, we enhanced the “network knowledge” in the rice N-regulatory network using transcriptome and gene interaction data from Arabidopsis and new data from Arabidopsis and rice plants exposed to the same N-treatment conditions. This cross-species network analysis uncovered a set of N-regulated transcription factors (TFs) predicted to target the same genes and network modules in both species. Supernode analysis of the TFs and their targets in these conserved network modules uncovered genes directly related to nitrogen use (e.g. N-assimilation) and to other shared biological processes indirectly related to nitrogen. This cross-species network approach was validated with members of two TF families in the supernode network, bZIP-TGA and HRS1/HHO family, have recently been experimentally validated to mediate the N-response in Arabidopsis.Fil: Obertello, Mariana. University of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; ArgentinaFil: Shrivastava, Stuti. University of New York; Estados UnidosFil: Katari, Manpreet S.. University of New York; Estados UnidosFil: Coruzzi, Gloria M.. University of New York; Estados Unido

    Modeling the global effect of the basic-leucine zipper transcription factor 1 (bZIP1) on nitrogen and light regulation in Arabidopsis

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    Background: Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of nitrogen (N) and light (L) treatment conditions and performing transcriptome analysis. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide. Results: This transcriptome analysis demonstrates that a mutation in the bZIP1 gene can alter the L and/or N-regulation of several gene clusters. More surprisingly, the bZIP1 mutation can also trigger N and/or L regulation of genes that are not normally controlled by these signals in WT plants. This analysis also reveals that bZIP1 can, to a large extent, invert gene regulation (e. g., several genes induced by N in WT plants are repressed by N in the bZIP1 mutant). Conclusion: These findings demonstrate that the bZIP1 mutation triggers a genome-wide de-regulation in response to L and/or N signals that range from i) a reduction of the L signal effect, to ii) unlocking gene regulation in response to L and N combinations. This systems biology approach demonstrates that bZIP1 tunes L and N signaling relationships genome-wide, and can suppress regulatory mechanisms hypothesized to be needed at different developmental stages and/or environmental conditions

    Interaction between Trichoderma pseudokoningii strains and the arbuscular mycorrhizal fungi Glomus mosseae and Gigaspora rosea

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    The interaction between Trichoderma pseudokoningii (Rifai) 511, 2212, 741A, 741B and 453 and the arbuscular mycorrhizal fungi Glomus mosseae (Nicol. & Gerd.) Gerdemann & Trappe BEG12 and Gigaspora rosea Nicolson & Schenck BEG9 were studied in vitro and in greenhouse experiments. All T. pseudokoningii strains inhibited the germination of G. mosseae and Gi. rosea except the strain 453, which did not affect the germination of Gi. rosea. Soluble exudates and volatile substances produced by all T. pseudokoningii strains inhibited the spore germination of G. mosseae. The germination of Gi. rosea spores was inhibited by the soluble exudates produced by T. pseudokoningii 2212 and 511, whereas T. pseudokoningii 714A and 714B inhibited the germination of Gi. rosea spores by the production of volatile substances. The strains of T. pseudokoningii did not affect dry matter and percentage of root length colonization of soybean inoculated with G. mosseae, except T. pseudokoningii 2212, which inhibited both parameters. However, all T. pseudokoningii strains decreased the shoot dry matter and the percentage of AM root length colonization of soybean inoculated with Gi. rosea. The saprotrophic fungi tested seem to affect AM colonization of root by effects on the presymbiotic phase of the AM fungi. No influence of AM fungi on the number of CPUs of T. pseudokoningii was found. The effect of saprotrophic fungi on AM fungal development and function varied with the strain of the saprotrophic species tested.Fil: Martinez, Alicia Elba. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Obertello, Mariana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Ocampo, Juan A.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Godeas, Alicia Margarita. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

    Actinorhizal nitrogen fixing nodules: infection process, molecular biology and genomics

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    Actinorhizal hosts are non-leguminous perennial plants belonging to 8 angiosperm families. They are capable of forming root nodules as a result of infection by a nitrogen-fixing actinomycete called Frankia. Actinorhizal nodules consist of multiple lobes, each of which represents a modified lateral root with infected cells in the expanded cortex. This article summarizes the most recent knowledge about this original symbiotic process. The infection process is described both at cytological and molecular levels. The use of transgenic Casuarinaceae for studying in actinorhizal nodules the regulation of several symbiotic promoters from legumes is also discussed. With progress in plant genome sequencing, comparative genomics in legumes and actinorhizal plants should contribute to the understanding of the evolutionary history of nitrogen-fixing symbioses. Key words : Nitrogen-fixation, actinorhizal nodules, Frankia, Casuarina, symbiotic gene. African Journal of Biotechnology Vol. 2 (12), pp. 528-538, December 200

    Interactions between Frankia BCU110501 (actinorhiza) and Gigaspora rosea (arbuscular mycorrhiza) with Discaria trinervis studied by spot inoculation

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    Root endosymbioses are associations between plants and soil microorganisms which contribute worldwide to plant nutrition and fitness. The most common associations is with arbuscular mycorrhizal fungi (AMF) and this is formed by the majority of land plants with fungi belonging to the Glomeromycota. Another important association is that formed by the Gram-positive actinobacteria, Frankia, and actinorhizal plants such as Discaria trinervis. In this paper we studied the effect of dual symbioses resulting from spot inoculation. This method was developed and tested to ensure actinorhizal nodule development only at the site of inoculation. Our results show that both symbioses can coexist and that AMF and Frankia can stimulate plant growth both as a single inoculum and in combination. Moreover, we suggest that there is no interference between the regulation of actinorhizal and arbuscular mycorrhizal symbiosis in D. trinervis, and that there is a synergistic effect on plant growth following the development of both symbioses.Fil: Obertello, Mariana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; ArgentinaFil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentin

    Estudio de un factor de transcripción MYB involucrado en la asimilación de nitrógeno en Arabidopsis y arroz

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    Nuestro objetivo a largo plazo es poder formular estrategias para la manipulación de reguladores claves de redes reguladas por nitrógeno, para lograr una incorporación y una asimilación más eficientes del nitrógeno en plantas de interés agronómico (ej. arroz). El nitrógeno es un elemento limitante para el crecimiento de las plantas. A partir del análisis de microarreglos de plántulas de arroz tratadas con nitrato/amonio por dos horas, se identificó genes candidatos que podrían modular y amplificar la respuesta a dicho nutriente. En este contexto se eligió inicialmente un factor de transcripción de la familia MYB, cuya expresión se vio altamente correlacionada con genes involucrados en la vía de la asimilación de nitrógeno. El estudio de su gen ortólogo en Arabidopsis thaliana sugiere que este TF estaría regulando positivamente la expresión de algunos genes claves en la asimilación del nitrógeno a nivel transcripcional (nitrato reductasa y glutamato sintasa). Además, utilizando dos líneas mutantes de Arabidopsis, se comprobó que la ausencia de dicho factor de transcripción repercutiría negativamente en el desarrollo de la raíz principal, contenido de nitrógeno total en semilla y, largo y numero de semillas por silicua cuando estas son crecidas en un medio con nitrógeno, comparado con wild-type. Estos resultados sugieren que este factor de transcripción tiene un rol importante en la respuesta mediada por nitrógeno.Fil: Romei, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaJornadas Exactas y el Agro: Aportes a la actividad agropecuaria y agroindustrialCiudad Autónoma de Buenos AiresArgentinaUniveridad de Buenos Aires. Facultad de Ciencias Exactas y Naturale

    Characterization of HHO5, a transcription factor involved in nitrogen response in A. thaliana and rice

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    Nitrogen deficiencies and the use of fertilizers is a major issue for crops production having both economic and environmental consequences. In a previous work, we have demonstrated that HHO5 gene is expressed in both A. thaliana and O. sativa when the plant is supplied with a nitrogen source and that plays an important role in the nitrogen response regulatory network with a systems biology perspective (Obertello et al., 2015). Our goal is to characterize HHO5 and to understand its role in nitrogen response pathway in both species. . Our in silico analysis of HHO5 endogen promoter compared with data of genes expressed under N supply conditions in both species revealed possible associations of HHO5 and regulatory pathways other than nitrogen use. We found sites targeted by known TFs associated with stress response, such as ABI5 and OsWRKY11 with two and three target sites respectively in OsHHO5 promoter and AtWRKY28 with two sites in AtHHO5 promoter. Based on that analysis, we selected different length of endogenous pAtHHO5 promoters in order to study its regulation and expression pattern in a N context. We generated several transgenic lines in A. thaliana using YFP and GUS as reporters genes. Also, in order to analyze HHO5 localization and its effects in N use efficiency, we generated O. sativa transgenic 35Sp::OsHHO5-GFP overexpressing lines.Fil: Romei, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaXXXIII Reunión Argentina de Fisiología Vegetal.Santa FeArgentinaSociedad Argentina de Fisiología Vegeta
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