Perfil de isolamento microbiano em cães com otite média e externa associadas Profile of microorganisms isolated from dogs with associated media and extern otitis

Comparou-se o perfil de isolamento microbiano de amostras coletadas de cães com otite média (OM) e externa (OE) associadas. Sessenta e quatro cães com otite média e externa foram avaliados durante 10 meses. Amostras dos condutos auditivos externos foram coletadas com auxílio de swabs estéreis e aquelas do ouvido médio pela técnica cirúrgica da osteotomia da bula timpânica. Os microrganismos foram cultivados e identificados de acordo com os métodos previamente descritos, e a susceptibilidade a antimicrobianos avaliada pelo método de difusão em ágar estabelecido pelo National Committee for Clinical Laboratory Standards. Bactérias anaeróbias estritas não foram isoladas em qualquer amostra. Todas as amostras de OE mostraram crescimento bacteriano e/ou fúngico. Na OM, a positividade nas culturas foi de 48%. Os microrganismos mais isolados na OE foram: Bacillus sp. (26,9%), M. pachydermatis (22,2%) e S. intermedius (21,7%); na OM foram: S. intermedius (32,5%), S. aureus subsp. aureus (22,5%) e bacilos Gram-negativos não fermentadores (10,0%). Observou-se alguma diferença no perfil de isolamento entre os quadros de OM e OE em 96,7% dos animais. Verificou-se elevada resistência de cepas de S. intermedius à penicilina G, ampicilina, eritromicina, tetraciclina e clindamicina.The isolation pattern of samples collected from dogs with both media (OM) and extern otitis (OE) was compared. Sixty-four dogs suffering from those conditions were studied over a 10-month period. Samples from the external ear were collected with sterile swabs while those from the middle ear were collected by osteotomy of the tympanic bulla. The microorganisms were cultured and identified according to methods previously described and to the susceptibility of antimicrobials according to the National Committee for Clinical Laboratory Standards agar diffusion test. Anaerobic bacteria were not isolated in any sample. All samples from OE showed bacterial and/or fungal growth. Concerning media otitis, this percentage was 48%. The most common microorganisms isolated from OE were Bacillus sp. (26.9%), M. pachydermatis (22.2%) and S. intermedius (21.7%) and those from OM were S. intermedius (32.5%), S. aureus subsp. aureus (22.5%) and non-fermentative Gram-negative rods (10.0%). Some difference was observed in the isolation pattern between media and extern otitis in 96.7% of the animals. High resistance rates of S. intermedius strains to penicillin G, ampicillin, erythromycin, tetracycline and clindamycin were found

Susceptibilidade a antimicrobianos de bactérias isoladas de otite externa em cães Antimicrobial sensitivity of bacteria from otitis externa in dogs

The occurrence of canine externa otitis in Fortaleza-Ceará is reported. About 91.5% of the animals with clinical signs were positive to bacterial culture. Among all infections, 49.5% were mixed infections and the most common pathogens were Staphylococcus spp coagulase negative or positive and Pseudomonas aeruginosa. The most effective antimicrobials for Staphylococcus coagulase negative were: the quinolones, the aminoglicoside netilmycin and the beta-lactams, excepted ampicillin, penicillin and oxacilin; for Staphylococcus coagulase positive were: cefotoxin, amoxicillin + clavulanic acid, imipenem, netilmycin and cephatoxin; for Pseudomonas aeruginosa were: ciprofloxacin, tobramycin and imipenem

Characterization And Selection Of Passion Fruit (yellow And Purple) Accessions Based On Molecular Markers And Disease Reactions For Use In Breeding Programs

Passiflora edulis Sims, which is native to South America, stands out as a passion fruit species with major potential for fruit production and marketing. This species is popularly known as yellow or purple passion fruit, depending on the color of the fruits produced. Brazil is the major worldwide producer of passion fruit; however, the average productivity of the country is low compared with its potential for culture. Fungal, bacterial and viral pathogens are among the factors limiting the productivity of passion fruit. Furthermore, no existing cultivars exhibit both productivity and resistance to disease. To select genetic material that will be useful for core collections and for increasing the genetic resistance of passion fruit cultivars to pathogens, we characterized 36 accessions based on 23 microsatellite loci and six variables related to the reactions to three diseases (woodiness virus, scab and anthracnose). We identified 127 alleles (an average of 5.52 alleles per locus), 30 % of which were private for yellow or purple passion fruit accessions. Analysis of variance and mean comparison tests indicated differences in five of the six variables (p < 0.05, Scott-Knott test). Differences between the average reactions of the yellow and purple passion fruit accessions were also observed for the symptoms of woodiness virus and anthracnose (p < 0.05, Mann-Whitney test).Together with these results, molecular and phenotypic estimates allowed the identification of groups of preferential accessions for use in breeding programs, for example, accessions BGP029, 071, 168, 205 and 277. © 2014 The Author(s)

Plasmid-mediated extended-spectrum beta-lactamase-producing strains of Enterobacteriaceae isolated from diabetes foot infections in a Brazilian diabetic center

We bacteriologically analyzed 156 species of Enterobacteriaceae, isolated from 138 patients with community-acquired diabetic foot ulcers, in a prospective study made at a diabetic center and at the Federal University of Ceará, Brazil, from March, 2000, to November, 2001.The samples were cultured using selective media, and identification, susceptibility tests and detection of plasmid-mediated-extended-spectrum-beta-lactamase (ESBL) producing strains were made with conventional and automated methods. The most frequently occurring pathogens were K. pneumoniae (21.2%), Morganella morganii (19.9%) and E. coli (15.4%). High resistance rates were noted for ampicillin, first generation cephalosporin, trimethoprim/sulfamethoxazole, tetracycline, amoxicillin-clavulanic acid and chloramphenicol. ESBL-producing strains were detected in 6% of the patients. Resistance among gram-negative bacteria has become increasingly common, even in community-acquired infections

Plasmid-mediated extended-spectrum beta-lactamase-producing strains of Enterobacteriaceae isolated from diabetes foot infections in a Brazilian diabetic center

We bacteriologically analyzed 156 species of Enterobacteriaceae, isolated from 138 patients with community-acquired diabetic foot ulcers, in a prospective study made at a diabetic center and at the Federal University of Ceará, Brazil, from March, 2000, to November, 2001.The samples were cultured using selective media, and identification, susceptibility tests and detection of plasmid-mediated-extended-spectrum-beta-lactamase (ESBL) producing strains were made with conventional and automated methods. The most frequently occurring pathogens were K. pneumoniae (21.2%), Morganella morganii (19.9%) and E. coli (15.4%). High resistance rates were noted for ampicillin, first generation cephalosporin, trimethoprim/sulfamethoxazole, tetracycline, amoxicillin-clavulanic acid and chloramphenicol. ESBL-producing strains were detected in 6% of the patients. Resistance among gram-negative bacteria has become increasingly common, even in community-acquired infections