17 research outputs found
Morfologia do estômago do cervo-do-pantanal (Blastocerus dichotomus, Illiger 1815)
Em razão da falta de informações sobre a morfologia do cervo-do-pantanal, objetivou-se apresentar a morfologia das câmaras gástricas desse cervídeo. Macroscopicamente, o estômago do cervo-do-pantanal é formado pelo rúmen, retículo, omaso e abomaso, assemelhando-se aos ruminantes domésticos. Microscopicamente, o rúmen e o abomaso são semelhantes aos animais domésticos, já o retículo e o omaso apresentam características específicas, como acentuada queratinização no ápice das pequenas projeções epiteliais do retículo e pregas omasais revestidas por discretas papilas
Farelo de algodão em rações para suínos nas fases de crescimento e terminação
Forty-eight pigs with 63 day olds and average
weight of 23.40±1.3 kg, were allotted in a
randomized block design with three treatments
(0%, 10% and 20% of cottonseed meal (FA) in
diets) and eight replicates with two pigs (one
barrow and one gilt). The blocks were used to
control changes in initial body weight. Experimen-
tal period was the growth phase (63 to 106 days
old) and growing-finishing (63 to 147 days old). In
each phase the diets were isonutritives, varying
however the levels of crude fiber. The levels of
cottonseed meal (FA) did not affect (p>0.05) the
performance of animals during the growth phase
or during the total period of evaluation (growing-
finishing). The qualitative parameters of manure
indicated that the FA determined decrease in the
nitrogen and phosphorus content, but only in the
growth phase. In the finishing phase the increase
in the FA inclusion promoted an increase in dry
matter content. Although FA can be used in up to
20% in diets for pigs in the growing and finishing
phase, it is necessary to include L-lysine and
soybean oil which causes an increase in cost per
kg produced pig.Quarenta e oito suínos, de 63 dias e peso de
23,40±1,3 kg, foram distribuídos em um delinea-
mento experimental em blocos casualizados, com
três tratamentos (0%, 10% e 20% de inclusão de
farelo de algodão -FA- nas rações) e oito
repetições compostas de dois animais (um macho
castrado e uma fêmea). Os blocos foram utiliza-
dos para controlar variações no peso corporal
inicial. O período experimental compreendeu a
fase de crescimento (63 a 106 dias) e crescimento-
terminação (63 a 147 dias de idade). Em cada uma
das fases as rações foram isonutritivas, varian-
do, entretanto, os níveis de fibra bruta. Os níveis
de FA não afetaram o desempenho dos animais.
A avaliação qualitativa dos dejetos indicou que o
FA determinou redução nos teores de fósforo e
nitrogênio, mas apenas na fase de crescimento.
Na fase de terminação o aumento na inclusão de
FA promoveu aumento no teor de matéria seca dos
dejetos. O FA pode ser utilizado em até 20% nas
rações para suínos na fase de crescimento e
terminação, porém a necessidade de inclusão de
L-lisina e óleo de soja determina aumento no custo/
kg de suíno produzido
Cancer chemopreventive activity of naphthoquinones and their analogs from Avicennia plants
10.1016/S0304-3835(01)00707-8Cancer Letters1742135-139CALE
Effect of Zn Substitution on the Structural and Physical Properties of Delafossite-Type Oxide CuCrO2
Resistivity Minimum Behavior and Weak Magnetic Disorder Characteristics in La2/3Ca1/3MnO3 Manganites
Association of hypomethylation of LINE-1 repetitive element in blood leukocyte DNA with an increased risk of hepatocellular carcinoma
Global DNA hypomethylation has been associated with increased risk for cancers of the colorectum, bladder, breast, head and neck, and testicular germ cells. The aim of this study was to examine whether global hypomethylation in blood leukocyte DNA is associated with the risk of hepatocellular carcinoma (HCC). A total of 315 HCC cases and 356 age-, sex- and HBsAg status-matched controls were included. Global methylation in blood leukocyte DNA was estimated by analyzing long interspersed element-1 (LINE-1) repeats using bisulfite-polymerase chain reaction (PCR) and pyrosequencing. We observed that the median methylation level in HCC cases (percentage of 5-methylcytosine (5mC)=77.7%) was significantly lower than that in controls (79.5% 5mC) (P=0.004, Wilcoxon rank-sum test). The odds ratios (ORs) of HCC for individuals in the third, second, and first (lowest) quartiles of LINE-1 methylation were 1.1 (95% confidence interval (CI) 0.7–1.8), 1.4 (95% CI 0.8–2.2), and 2.6 (95% CI 1.7–4.1) (P for trend <0.001), respectively, compared to individuals in the fourth (highest) quartile. A 1.9-fold (95% CI 1.4–2.6) increased risk of HCC was observed among individuals with LINE-1 methylation below the median compared to individuals with higher (>median) LINE-1 methylation. Our results demonstrate for the first time that individuals with global hypomethylation measured in LINE-1 repeats in blood leukocyte DNA have an increased risk for HCC. Our data provide the evidence that global hypomethylation detected in the easily obtainable DNA source of blood leukocytes may help identify individuals at risk of HCC
CD9 Is Associated with Leukemia Inhibitory Factor-mediated Maintenance of Embryonic Stem Cells
Mouse embryonic stem (ES) cells can proliferate indefinitely in an undifferentiated state in the presence of leukemia inhibitory factor (LIF), or differentiate into all three germ layers upon removal of this factor. To determine cellular factors associated with self-renewal of undifferentiated ES cells, we used polymerase chain reaction-assisted cDNA subtraction to screen genes that are expressed in undifferentiated ES cells and down-regulated after incubating these cells in a differentiation medium without LIF for 48 h. The mRNA expression of a tetraspanin transmembrane protein, CD9, was high in undifferentiated ES cells and decreased shortly after cell differentiation. An immunohistochemical analysis confirmed that plasma membrane-associated CD9 was expressed in undifferentiated ES cells but low in the differentiated cells. Addition of LIF to differentiating ES cells reinduced mRNA expression of CD9, and CD9 expression was accompanied with a reappearance of undifferentiated ES cells. Furthermore, activation of STAT3 induced the expression of CD9, indicating the LIF/STAT3 pathway is critical for maintaining CD9 expression. Finally, addition of anti-CD9 antibody blocked ES cell colony formation and reduced cell viability. These results indicate that CD9 may play a role in LIF-mediated maintenance of undifferentiated ES cells