A Novel Derivatization Ultraviolet Spectrophotometric Method for the Determination of Dihydroartemisinin using p- Nitroaniline

Purpose: To develop a novel ultraviolet (UV)–spectrophotometric method for the determination of dihydroartemisinin (DHA) in tablets using  p-nitroaniline (PNA) as a derivatizing agent.Methods: Derivatization was based on the reaction between methanol solutions of dihydroartemisinin (DHA) and p-nitroaniline (PNA) in acid medium (1M HCI) at elevated temperature and for a short reaction time. Optimal detector response was obtained within 15 min when the reaction was carried out at 90 0C in a molar ratio of 2:1 (DHA:PNA). The method used for analysis was validated and a linear calibration curve constructed in the range of 30 – 100 ìg/mL for the reaction mixture at an absorbanceof 290 nm.Results: Separation of adduct from PNA was better achieved on reversed phase thin layer chromatography (TLC) using acetonitrile : water (60:50) or on high performance liquid chromatography (HPLC) with retention times of 2.8 min for PNA and 5.8 min for the adduct. The limit of detection was 6ìg/mL. The method was precise and accurate in the range 100.70 - 100.96 %, with intraday and interday precisions of less than 2 % at concentrations of 40 and 80 ìg/mL, respectively. The new method was applied to the assay of two brands of dihydroartemisinin tablets with accuracy similar tothat of the International Pharmacopoeia (IP) UV-spectrophotometric method (p > 0.05).Conclusion: The derivatization method is simple, direct, devoid of dilutions and inexpensive in terms of reagent requirements and analyte volume, and has a shorter reaction time, cpmpared with IP method. Based on the foregoing, the method can be adopted as an alternative to the official assay method for routine quality control of dihydroartemisinin tablets.Keywords: Derivatization, Ultraviolet spectrophotometry,  Dihydroartemisinin, p-Nitroaniline, Analysis, Assay, Quality contro

Evaluation of Revenue Channels and Challenges in Sustainable Management of Oyo State Forest Reserves, Nigeria

Forest revenue system is an instrument used by government to achieve various goals and objectives in forest management. This paper evaluates the various challenges of revenue collection and remittance in Oyo forestry service in Nigeria. A total of 94 forest officials were identified and reached (100%) in all the forestry administrative zones in Oyo State, including the headquarters through a set of structured questionnaire and data obtained were analyzed using descriptive statistics and logit regression at \u3b10.05. Theaverage age of the respondents was 39.7\ub16.5 years, mostly male (71%), married (75.6%) and had secondary education (63%). Challenges confronting adequate revenue collection included lack of proper law enforcement, payment of cash by revenue collectors and inability of field officers to withstand armed illegal fellers with odds-ratio of 55694.85, 668.78 and 20.79 respectively. Problems facing adequate revenue remittance were remittance by field staff, inaccessibility of bank on time, lack of provision of incentives for running cost by the government and possibilities of field officers indulging in printing of fake receipts with odds-ratio of 140.18, 116.80, 3.65 and 3.44 respectively. The study exposed the various challenges facing adequate revenue collection and remittance in Oyo State forestry service, it is therefore necessary for the forest managers in the State to utilize this information wisely for the betterment of revenue generation

Help-seeking behavior among women currently leaking urine in Nigeria: is it any different from the rest of the world?

Babatunde O Adedokun,1 Imran O Morhason-Bello,2 Oladosu A Ojengbede,2 Ngozi S Okonkwo,1 Charles Kolade21Department of Epidemiology, Medical Statistics, and Environmental Health, College of Medicine, University of Ibadan, Oyo state, Nigeria; 2Department of Obstetrics and Gynecology, College of Medicine/University College Hospital, University of Ibadan, Ibadan, Oyo state, NigeriaObjective: We examined help-seeking behaviors and factors influencing their choice of hospital care in women currently leaking urine.Materials and methods: This study was part of a multistage community survey conducted among 5001 women in Nigeria who participated in the Ibadan Urinary Incontinence Household Survey. Help-seeking behavior was analyzed among 139 respondents currently leaking urine within the population surveyed.Results: The mean age of those currently leaking urine was 35.7 years (standard deviation = 15.8). Only 18 (12.9%) had ever sought help, of which 15 had received hospital care. Logistic regression analysis showed that the odds of seeking hospital care was higher among less educated women (odds ratio [OR] = 4.05, 95% confidence interval [CI]: 1.17–13.89) and among those with severe incontinence (OR = 4.20, 95% CI: 1.24–14.29). Reasons mentioned for not seeking hospital care include a belief that the condition is not life-threatening (51.2%), do not believe there is treatment (18.2%), lack of funds (1.7%), too shy to disclose (2.5%), afraid of complications (1.7%), other (2.5%), and no reason (22.3%).Conclusion: This study shows that very few women, currently experiencing urinary incontinence have sought medical care (approximately 1 in 10); and that the barriers identified are similar to those identified in previous studies, except that these women lack the necessary funds to seek care.Keywords: urinary incontinence, help (health)-seeking behavior, women, Nigeri

Clinicohistopathological Analysis of 5 Nigerian cases of Malignant Fibrous Histiocytoma of the Jaws.

Background: Malignant fibrous histiocytoma (MFH), is a soft tissue sarcoma that occurs predominantly in the soft tissue of the extremities. It rarely occurs in facial bones. Few cases in the jaws have been reported.Objective: To report and review the relevant clinicopathologic features of 5 cases of jaw MFH in Nigerians.Methods: All cases in the records of the Department of Oral Pathology of the Lagos University Teaching Hospital which were histologically diagnosed as MFH were retrieved. Hematoxylin and eosin slides of cases were re-examined to confirm diagnosis. Information retrieved included age ,sex, location , x-ray , estimated duration , recurrence, and histological presentation. Data was analyzed using SPSS statistical package.Results: Age ranged between 12- 42 years with a male sex predilection (3 cases/ 60.0%) and sole maxillary site predilection(5cases /100.0%). Estimated duration of lesion ranged from 2 -12 months. Two cases recurred post surgical treatment. All cases presented as the storiformpleomorphic type with one case presenting with a delicate / scanty connective tissue stroma. Chronic inflammation was scanty in the two cases that recurred.Conclusion: MFH of the jaws is rare among Nigerians. Its clinico radiological presentation maymimick other more aggressive or less benign lesions of the jaws thereby resulting in misdiagnosis and in appropriate patient management.Key words: Malignant; fibrous ;histiocytoma, Nigeri