ISOLATION, CHARACTERIZATION, AND ASSESSMENT OF CYTOTOXICITY OF ISOFLAVONES DERIVED FROM KUDZU ROOT AND SOY MOLASSES

We acknowledge the financial support of the Russian Science Foundation, Grant No.20-66-47017

Architecture of composite multilayer semiconductor nanostructures

The problem of ensuring the operational parameters of composite multilayer semiconductor nanoscale structures at the design technology stages is solved. A mathematical model based on the physics of processes occurring in the structure during operation is developed. The problem is solved for the resonant-tunnelling AlGaAs nanoheterostructures

The influense of overexpression of gene KLOTHO on the growth characteristics of cell cultures of glioma and colon cancer

This article represents the results of research conducted on the cultures of glioma and cell culture of colon cancer (A-172 and Caco-2, respectively). In cells induced the overexpression of the gene Klotho, after which it was observed a significant decrease of the number of parameters of tumor cells.В статье представлены результаты исследований, которые были проведены на клеточных культурах глиомы человека и рака ободочной кишки (линии A-172 и Caco-2 соответственно). В клетках индуцировали гиперэкспрессию гена Klotho, после чего было отмечено достоверное снижение ряда параметров опухолевых клето

Research the action of complexly modulated electromagnetic field on the culture of fibroblast

Research the action of complexly modulated electromagnetic field on the culture of fibroblastИсследование действия сложномодулированного электромагнитного поля на культуру фибробластов

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses Against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This work was supported by the Russian Science Foundation, Grant No. 20-66-47017

Philosophical cognition of the retro-causal –epigenetic landscape in atopic dermatitis

The purpose of the study is to reveal retrocausality and show importance of introducing a different approach, such as epigenetic in the diagnosis and treatment of atopic dermatitis.Цель исследования – раскрыть ретропричинность и показать значимость внедрения иного подхода, такого как эпигенетический, в диагностике и лечении атопического дерматита

Using stem cells to treat liver diseases

The aim of the study – to evaluate the study of mesenchymal stem cells.Цель исследования – изучение функций мезенхимальных стволовых клеток

The influence of the environment on the formation of allergies

The purpose of the study is to identify the correlation between the state of the environment and the development of allergic diseases.Цель исследования – выявить зависимость между состоянием окружающей среды и развитием аллергических заболеваний