Estudo do comportamento térmico dinâmico-mecânico de blendas de biopoliamidas (PA610/PA1010) / Dynamic mechanical and thermal behavior study of biopolyamid Blends (PA610 / PA1010)

As biopoliamidas possuem cadeias poliméricas de diversos comprimentos e essa variação no comprimento da cadeia pode permitir à substituição das poliamidas atuais a base de petróleo, incluindo as poliamidas de cadeia curta (PA6, PA66) como também substituir suas parentes de cadeia longa (PA12). Algumas mudanças, porém, podem ser necessárias em sua composição para atender exigências bem específicas, dependendo do produto e da sua aplicação. Nesse sentido, a formação de blendas permite obter materiais com propriedades intermediárias a dos componentes puros, através de um custo de desenvolvimento relativamente menor do que sintetizar um material polimérico completamente novo. Este trabalho busca estudar blendas formadas por biopoliamidas (PA610 e PA1010) derivadas do óleo de mamona, e as propriedades térmicas dinâmico-mecânicas (DMTA) das diferentes combinações foram avaliadas para entender o comportamento dos materiais no composto (miscibilidade). 

Systematic Study of Short Range Antiferromagnetic Order and The Spin-Glass State in Lightly Doped La2-xSrxCuO4

Systematic measurements of the magnetic susceptibility were performed on single crystals of lightly doped La2-xSrxCuO4 (x=0.03, 0.04 and 0.05). For all samples the temperature dependence of the in-plane magnetic susceptibility shows typical spin-glass features with spin-glass transition temperatures Tg of 6.3K, 5.5K and 5.0K for x=0.03, 0.04 and 0.05, respectively. The canonical spin-glass order parameter extracted from the in-plane susceptibility of all the samples follows a universal scaling curve. On the other hand, the out-of-plane magnetic susceptibility deviates from Curie law below a temperature Tdv, higher than Tg. Comparing with previous neutron scattering results with an instrumental energy resolution of 0.25 meV from Wakimoto et al., the x-dependence of Tdv is qualitatively the same as that of Tel, the temperature below which the elastic magnetic scattering develops around (pi, pi). Thus, a revised magnetic phase diagram in the lightly doped region of La2-xSrxCuO4 is proposed. The Curie constants calculated from the in-plane susceptibility are independent of the Sr concentration. On the basis of the cluster spin-glass model, this fact might reflect an inhomogeneous distribution of doped holes in the CuO2 plane, such as in a stripe structure.Comment: 7 pages, 6 figure

The GATA1s isoform is normally down-regulated during terminal haematopoietic differentiation and over-expression leads to failure to repress MYB, CCND2 and SKI during erythroid differentiation of K562 cells

Background: Although GATA1 is one of the most extensively studied haematopoietic transcription factors little is currently known about the physiological functions of its naturally occurring isoforms GATA1s and GATA1FL in humans—particularly whether the isoforms have distinct roles in different lineages and whether they have non-redundant roles in haematopoietic differentiation. As well as being of general interest to understanding of haematopoiesis, GATA1 isoform biology is important for children with Down syndrome associated acute megakaryoblastic leukaemia (DS-AMKL) where GATA1FL mutations are an essential driver for disease pathogenesis. <p/>Methods: Human primary cells and cell lines were analyzed using GATA1 isoform specific PCR. K562 cells expressing GATA1s or GATA1FL transgenes were used to model the effects of the two isoforms on in vitro haematopoietic differentiation. <p/>Results: We found no evidence for lineage specific use of GATA1 isoforms; however GATA1s transcripts, but not GATA1FL transcripts, are down-regulated during in vitro induction of terminal megakaryocytic and erythroid differentiation in the cell line K562. In addition, transgenic K562-GATA1s and K562-GATA1FL cells have distinct gene expression profiles both in steady state and during terminal erythroid differentiation, with GATA1s expression characterised by lack of repression of MYB, CCND2 and SKI. <p/>Conclusions: These findings support the theory that the GATA1s isoform plays a role in the maintenance of proliferative multipotent megakaryocyte-erythroid precursor cells and must be down-regulated prior to terminal differentiation. In addition our data suggest that SKI may be a potential therapeutic target for the treatment of children with DS-AMKL

Impact of rs361072 in the Phosphoinositide 3-Kinase p110β Gene on Whole-Body Glucose Metabolism and Subunit Protein Expression in Skeletal Muscle

OBJECTIVE-Phosphoinositide 3-kinase (PI3K) is a major effector in insulin signaling. rs361072, located in the promoter of the gene (PIK3CB) for the p110 beta subunit, has previously been found to be associated with homeostasis model assessment for insulin resistance (HOMA-IR) in obese subjects. The aim was to investigate the influence of rs361072 on in vivo glucose metabolism, skeletal muscle PI3K subunit protein levels, and type 2 diabetes. RESEARCH DESIGN AND METHODS-The functional role of rs361072 was studied in 196 Danish healthy adult twins. Peripheral and hepatic insulin sensitivity was assessed by a euglycemic-hyperinsulinemic clamp. Basal and insulin-stimulated biopsies were taken from the vastus lateralis muscle, and tissue p110 beta and p85 alpha proteins were measured by Western blotting. The genetic association with type 2 diabetes and quantitative metabolic traits was investigated in 9,316 Danes with glucose tolerance ranging from normal to overt type 2 diabetes. RESULTS-While hepatic insulin resistance was similar in the fasting state, carriers of the minor G allele had lower hepatic glucose output (per-allele effect: 16%, P-add = 0.004) during high physiological insulin infusion. rs361072 did not associate with insulin-stimulated peripheral glucose disposal despite a decreased muscle p85 alpha:p110 beta protein ratio (P-add = 0.03) in G allele carriers. No association with HOMA-IR or type 2 diabetes (odds ratio 1.07, P = 0.5) was identified, and obesity did not interact with rs361072 on these traits. CONCLUSIONS-Our study suggests that the minor G allele of PIK3CB rs361072 associates with decreased muscle p85 alpha:p110 beta ratio and lower hepatic glucose production at high plasma insulin levels. However, no impact on type 2 diabetes prevalence was found. Diabetes 59:1108-1112, 201

Dynamics of the Volterra-type integral and differentiation operators on generalized Fock spaces

[EN] Various dynamical properties of the differentiation and Volterra-type integral operators on generalized Fock spaces are studied. We show that the differentiation operator is always supercyclic on these spaces. We further characterize when it is hypercyclic, power bounded and uniformly mean ergodic. We prove that the operator satisfies the Ritt's resolvent condition if and only if it is power bounded and uniformly mean ergodic. Some similar results are obtained for the Volterra-type and Hardy integral operators.J. Bonet was partially supported by the research projects MTM2016-76647-P and GV Prometeo 2017/102 (Spain). M. Worku is supported by ISP project, Addis Ababa University, Ethiopia.Bonet Solves, JA.; Mengestie, T.; Worku, M. (2019). Dynamics of the Volterra-type integral and differentiation operators on generalized Fock spaces. Results in Mathematics. 74(4):1-15. https://doi.org/10.1007/s00025-019-1123-7S115744Abanin, A.V., Tien, P.T.: Differentiation and integration operators on weighted Banach spaces of holomorphic functions. Math. Nachr. 290(8–9), 1144–1162 (2017)Atzmon, A., Brive, B.: Surjectivity and invariant subspaces of differential operators on weighted Bergman spaces of entire functions, Bergman spaces and related topics in complex analysis, Contemp. Math., vol. 404, Amer. Math. Soc., Providence, RI, pp. 27–39 (2006)Bayart, F., Matheron, E.: Dynamics of Linear Operators, Cambridge Tracts in Math, vol. 179. Cambridge Univ. Press, Cambridge (2009)Bermúdez, T., Bonilla, A., Peris, A.: On hypercyclicity and supercyclicity criteria. Bull. Austral. Math. Soc. 70, 45–54 (2004)Beltrán, M.J.: Dynamics of differentiation and integration operators on weighted space of entire functions. Studia Math. 221, 35–60 (2014)Beltrán, M.J., Bonet, J., Fernández, C.: Classical operators on weighted Banach spaces of entire functions. Proc. Am. Math. Soc. 141, 4293–4303 (2013)Bès, J., Peris, A.: Hereditarily hypercyclic operators. J. Funct. Anal. 167, 94–112 (1999)Bonet, J.: Dynamics of the differentiation operator on weighted spaces of entire functions. Math. Z. 26, 649–657 (2009)Bonet, J.: The spectrum of Volterra operators on weighted Banach spaces of entire functions. Q. J. Math. 66, 799–807 (2015)Bonet, J., Bonilla, A.: Chaos of the differentiation operator on weighted Banach spaces of entire functions. Complex Anal. Oper. Theory 7, 33–42 (2013)Bonet, J., Taskinen, J.: A note about Volterra operators on weighted Banach spaces of entire functions. Math. Nachr. 288, 1216–1225 (2015)Constantin, O., Persson, A.-M.: The spectrum of Volterra-type integration operators on generalized Fock spaces. Bull. Lond. Math. Soc. 47, 958–963 (2015)Constantin, O., Peláez, J.-Á.: Integral operators, embedding theorems and a Littlewood–Paley formula on weighted Fock spaces. J. Geom. Anal. 26, 1109–1154 (2016)De La Rosa, M., Read, C.: A hypercyclic operator whose direct sum is not hypercyclic. J. Oper. Theory 61, 369–380 (2009)Dunford, N.: Spectral theory. I. Convergence to projections. Trans. Am. Math. Soc. 54, 185–217 (1943)Grosse-Erdmann, K.G., Peris Manguillot, A.: Linear Chaos. Springer, New York (2011)Harutyunyan, A., Lusky, W.: On the boundedness of the differentiation operator between weighted spaces of holomorphic functions. Studia Math. 184, 233–247 (2008)Krengel, U.: Ergodic Theorems. Walter de Gruyter, Berlin (1985)Lyubich, Yu.: Spectral localization, power boundedness and invariant subspaces under Ritt’s type condition. Studia Mathematica 143(2), 153–167 (1999)Mengestie, T.: A note on the differential operator on generalized Fock spaces. J. Math. Anal. Appl. 458(2), 937–948 (2018)Mengestie, T.: Spectral properties of Volterra-type integral operators on Fock–Sobolev spaces. J. Kor. Math. Soc. 54(6), 1801–1816 (2017)Mengestie, T.: On the spectrum of volterra-type integral operators on Fock–Sobolev spaces. Complex Anal. Oper. Theory 11(6), 1451–1461 (2017)Mengestie, T., Ueki, S.: Integral, differential and multiplication operators on weighted Fock spaces. Complex Anal. Oper. Theory 13, 935–95 (2019)Mengestie, T., Worku, M.: Isolated and essentially isolated Volterra-type integral operators on generalized Fock spaces. Integr. Transf. Spec. Funct. 30, 41–54 (2019)Nagy, B., Zemanek, J.A.: A resolvent condition implying power boundedness. Studia Math. 134, 143–151 (1999)Nevanlinna, O.: Convergence of iterations for linear equations. Lecture Notes in Mathematics. ETH Zürich, Birkhäuser, Basel (1993)Ritt, R.K.: A condition that $$\lim _{n\rightarrow \infty } n^{-1}T^n =0$$. Proc. Am. Math. Soc. 4, 898–899 (1953)Ueki, S.: Characterization for Fock-type space via higher order derivatives and its application. Complex Anal. Oper. Theory 8, 1475–1486 (2014)Yosida, K.: Functional Analysis. Springer, Berlin (1978)Yosida, K., Kakutani, S.: Operator-theoretical treatment of Marko’s process and mean ergodic theorem. Ann. Math. 42(1), 188–228 (1941

Desulfotomaculum varum sp. nov., a moderately thermophilic sulfate-reducing bacterium isolated from a microbial mat colonizing a Great Artesian Basin bore well runoff channel

A strictly anaerobic moderately thermophilic bacterium, designated strain RH04-3T (T = type strain), was isolated from a red colored microbial mat that colonizes a Great Artesian Basin (GAB) bore well (Registered Number 17263) runoff channel at 66 °C. The cells of strain RH04-3T were straight to slightly curved, sporulating, Gram-positive rods (2.0–5.0 × 1.0 μm) that grew optimally at 50 °C (temperature growth range between 37 and 55 °C) and at pH 7 (pH growth range of 5.0 and 8.5). Growth was inhibited by NaCl concentrations ≥1.5% (w/v), and by chloramphenicol, streptomycin, tetracycline, penicillin and ampicillin. The strain utilized fructose, mannose, glycerol, lactate, pyruvate and H2 in the presence of sulfate, and fermented pyruvate in the absence of sulfate. Strain RH04-3T reduced sulfate, sulfite, thiosulfate and elemental sulfur, but not nitrate, nitrite, iron(III), arsenate(V), vanadium(V) or cobalt(III) as terminal electron acceptors. The G + C content of DNA was 52.4 ± 0.8 mol % as determined by the thermal denaturation (Tm) method. 16S rRNA sequence analysis indicated that strain RH04-3T was a member of the genus Desulfotomaculum and was most closely related to Desulfotomaculum putei (similarity value of 95.2%) and Desulfotomaculum hydrothermale (similarity value of 93.6%). On the basis of phylogenetic and phenotypic characteristics, strain RH04-3T is considered to represent a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum varum sp. nov. is proposed. The type strain RH04-3T = JCM 16158T = KCTC 5794T

Complement activation capacity in plasma before and during high-dose prednisolone treatment and tapering in exacerbations of Crohn's disease and ulcerative colitis

BACKGROUND: Ulcerative colitis (UC) and Crohn's disease (CD) are characterized by intestinal inflammation mainly caused by a disturbance in the balance between cytokines and increased complement (C) activation. Our aim was to evaluate possible associations between C activation capacity and prednisolone treatment. METHODS: Plasma from patients with exacerbations of UC (n = 18) or CD (n = 18) were collected before and during high dose prednisolone treatment (1 mg/kg body weight) and tapering. Friedman's two way analysis of variance, Mann-Whitney U test and Wilcoxon signed-rank sum test were used RESULTS: Before treatment, plasma from CD patients showed significant elevations in all C-mediated analyses compared to the values obtained from 38 healthy controls (p < 0.02), and in mannan binding lectin (MBL)-concentration and MBL-C4-activation capacity (AC) values compared to UC patients (p < 0.02). Before treatment, plasma from UC patients showed significant elevations only in the classical pathway-mediated C3-AC compared to values obtained from healthy controls (p < 0.01). After treatment was initiated, significant reductions, which persisted during follow-up, were observed in the classical pathway-mediated C3-AC and MBL-C4-AC in plasma from CD patients (p < 0.05). CONCLUSION: Our findings indicate that C activation capacity is up-regulated significantly in plasma from CD patients. The decreases observed after prednisolone treatment reflect a general down-regulation in immune activation

Genome-Wide Analysis of Müller Glial Differentiation Reveals a Requirement for Notch Signaling in Postmitotic Cells to Maintain the Glial Fate

Previous studies have shown that Müller glia are closely related to retinal progenitors; these two cell types express many of the same genes and after damage to the retina, Müller glia can serve as a source for new neurons, particularly in non-mammalian vertebrates. We investigated the period of postnatal retinal development when progenitors are differentiating into Müller glia to better understand this transition. FACS purified retinal progenitors and Müller glia from various ages of Hes5-GFP mice were analyzed by Affymetrix cDNA microarrays. We found that genes known to be enriched/expressed by Müller glia steadily increase over the first three postnatal weeks, while genes associated with the mitotic cell cycle are rapidly downregulated from P0 to P7. Interestingly, progenitor genes not directly associated with the mitotic cell cycle, like the proneural genes Ascl1 and Neurog2, decline more slowly over the first 10–14 days of postnatal development, and there is a peak in Notch signaling several days after the presumptive Müller glia have been generated. To confirm that Notch signaling continues in the postmitotic Müller glia, we performed in situ hybridization, immunolocalization for the active form of Notch, and immunofluorescence for BrdU. Using genetic and pharmacological approaches, we found that sustained Notch signaling in the postmitotic Müller glia is necessary for their maturation and the stabilization of the glial identity for almost a week after the cells have exited the mitotic cell cycle