169 research outputs found
BCG-INDUCED PRO-INFLAMMATORY PHENOTYPE OF MESENCHYMAL STEM CELLS: EFFECT OF IMMUNE MODULATORS
In case of mycobacterial infection the granulomatous infiltration foci contain the significant amount of mesenchymal stem cells (MSC), which functional phenotype and respective function in anti-tuberculosis immune defense remain unknown.Goal of the study: to characterize the MSC phenotype, formed by their interaction with BCG of M. bovis and to evaluate the changes in this phenotype caused by the action of inhibitors and stimulants of immune regulatory action.Materials and methods: MSC retrieved from bone marrow of mice were cultured with the presence and absence of BCG of M. bovis and/or poludanum TLR3 agonist; and the effect of two latter on the production of pro- and anti-inflammatory cytokines was evaluated by enzyme multiplied immunoassay. Flow cytometry and radioactive testing were used to evaluate the impact of cultured BCG fluid and poludanum-conditioned MSC on the proliferative and apoptotic activity of splenocytes. The inhibitors of indoleamine 2,3-dioxygenase (IDO), cyclooxygenase-2 (COG-2) or NO synthase were added to certain cultures alone with BCG and poludanum, and the contributions of IDO, COG-2 and NO to BCG and poludanum-induced response were assessed.Results. Pro-inflammatory polarization of MSC under the action of BCG and poludanum was demonstrated. Pro-inflammatory MSC phenotype correlated to their anti-apoptogenic and growth-stimulating actions on the splenocytes. It was demonstrated that IDO and NO restrained BCG-induced polarization and conversely COG-2 promoted BCG-induced pro-inflammatory polarization of MSC.Conclusions. 1. MSC actively participate in the formation of immunologic anti-mycobacterial resistance. 2. Targeted regulation of IDO and NO production can be feasibly applied for formation of anti-tuberculous vaccinal immunity and control mycobacterial infection
Functional System of Creative Thinking
In the article on the basis of electroencephalographic and psychological indicators the concept the mechanism of creative thinking was formulated. Creative thinking as a process involves operating images and searching similar tasks in memory (mental representations). High result in creative thinking is possible under the condition of high decision-making and low level of critical-controlling program
Relationship Between The Rhythmic Activity Of The Cerebral Cortex And The Manifestation Of Impulsivity / Reflexivity
The most significant EEG differences were obtained by us for the purpose of receiving the indicators
of the coherence of the brain biopotentials in Ξ-, ΞΈ-, Ξ²-frequency bands. These EEG differences were obtained
in the course of performing tasks of the divergent type that are associated with the cognitive style of
"impulsivity-reflexivity", which in its turn is based on the ability of an individual to make quick or slow
decisions. It was established that in males, who are reflexive, the level of spatial synchronization was higher
than the one of impulsive men in the Ξ-, ΞΈ-, and Ξ²-frequency bands while performing the divergent task. And
this was mainly achieved due to closer long-distance connections of the frontal areas of the cerebral cortex
with occipital, temporal and parietal areas
Π§ΡΠ΅ΡΠΊΠΎΠΆΠ½Π°Ρ Π½Π΅ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½Π°Ρ Π²Π΅Π³Π΅ΡΠ°ΡΠΈΠ²Π½Π°Ρ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΡ: Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Ρ ΠΌΠ΅ΡΠΎΠ΄Π° Π² ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΡΡΠΎΠ² ΠΏΠΎ Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΠΌ Π±ΠΎΠ»Π΅Π·Π½ΡΠΌ
The article provides an overview of key studies on vagus nerve stimulation. The vagus nerve stimulation method affects the nerve fibers of the internal and external nerve plexuses, enhancing the parasympathetic effect. The positive therapeutic effect has been achieved in various experimental and clinical studies on the treatment of neurological and cardiac diseases, metabolic syndrome, and obesity. It has been shown that vagus nerve stimulation reduces epileptic seizures frequency, cardiac arrhythmias, and is prognostically favorable for the treatment of ischemia and reperfusion injury of the myocardium. Recent studies have examined the inflammatory reflex involved in the pathogenesis of obesity. The role of bioelectronic vagus nerve stimulation is being actively investigated for the treatment of obesity and metabolic syndrome.Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½ ΠΎΠ±Π·ΠΎΡ ΠΊΠ»ΡΡΠ΅Π²ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΠΈ Π±Π»ΡΠΆΠ΄Π°ΡΡΠ΅Π³ΠΎ Π½Π΅ΡΠ²Π°. ΠΠ°Π½Π½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ Π²Π»ΠΈΡΠ΅Ρ Π½Π° Π½Π΅ΡΠ²Π½ΡΠ΅ Π²ΠΎΠ»ΠΎΠΊΠ½Π° Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΡ
ΠΈ Π½Π°ΡΡΠΆΠ½ΡΡ
Π½Π΅ΡΠ²Π½ΡΡ
ΡΠΏΠ»Π΅ΡΠ΅Π½ΠΈΠΉ, ΡΡΠΈΠ»ΠΈΠ²Π°Ρ ΠΏΠ°ΡΠ°ΡΠΈΠΌΠΏΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅. ΠΠ»Π°Π³ΠΎΠ΄Π°ΡΡ ΡΠ°ΠΊΠΎΠΌΡ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
Π΄ΠΎΡΡΠΈΠ³Π½ΡΡ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
, ΠΊΠ°ΡΠ΄ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° ΠΈ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΡ Π±Π»ΡΠΆΠ΄Π°ΡΡΠ΅Π³ΠΎ Π½Π΅ΡΠ²Π° ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΏΡΠΈΡΡΡΠΏΠΎΠ² ΡΠΏΠΈΠ»Π΅ΠΏΡΠΈΠΈ, Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΡΠΈΡΠΌΠ° ΡΠ΅ΡΠ΄ΡΠ°, ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈ Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½Π° Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΈΡΠ΅ΠΌΠΈΠΈ ΠΈ ΡΠ΅ΠΏΠ΅ΡΡΡΠ·ΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ ΠΌΠΈΠΎΠΊΠ°ΡΠ΄Π°. Π Π½Π΅Π΄Π°Π²Π½ΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
ΠΈΠ·ΡΡΠ΅Π½ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠ΅ΡΠ»Π΅ΠΊΡ, ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΠΉ Π² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΡ. Π ΠΎΠ»Ρ Π±ΠΈΠΎΡΠ»Π΅ΠΊΡΡΠΎΠ½Π½ΠΎΠΉ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΠΈ Π±Π»ΡΠΆΠ΄Π°ΡΡΠ΅Π³ΠΎ Π½Π΅ΡΠ²Π° Π°ΠΊΡΠΈΠ²Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΡΡΡ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΡ ΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°
ΠΠ¦Π-ΠΠΠΠ£Π¦ΠΠ ΠΠΠΠΠΠ«Π ΠΠ ΠΠΠΠ‘ΠΠΠΠΠ’ΠΠΠ¬ΠΠ«Π Π€ΠΠΠΠ’ΠΠ ΠΠΠΠΠΠ₯ΠΠΠΠΠ¬ΠΠ«Π₯ Π‘Π’ΠΠΠΠΠΠ«Π₯ ΠΠΠΠ’ΠΠ: ΠΠΠΠ―ΠΠΠ ΠΠΠΠ£ΠΠΠΠΠΠ£ΠΠ―Π’ΠΠ ΠΠ
In case of mycobacterial infection the granulomatous infiltration foci contain the significant amount of mesenchymal stem cells (MSC), which functional phenotype and respective function in anti-tuberculosis immune defense remain unknown.Goal of the study: to characterize the MSC phenotype, formed by their interaction with BCG of M. bovis and to evaluate the changes in this phenotype caused by the action of inhibitors and stimulants of immune regulatory action.Materials and methods: MSC retrieved from bone marrow of mice were cultured with the presence and absence of BCG of M. bovis and/or poludanum TLR3 agonist; and the effect of two latter on the production of pro- and anti-inflammatory cytokines was evaluated by enzyme multiplied immunoassay. Flow cytometry and radioactive testing were used to evaluate the impact of cultured BCG fluid and poludanum-conditioned MSC on the proliferative and apoptotic activity of splenocytes. The inhibitors of indoleamine 2,3-dioxygenase (IDO), cyclooxygenase-2 (COG-2) or NO synthase were added to certain cultures alone with BCG and poludanum, and the contributions of IDO, COG-2 and NO to BCG and poludanum-induced response were assessed.Results. Pro-inflammatory polarization of MSC under the action of BCG and poludanum was demonstrated. Pro-inflammatory MSC phenotype correlated to their anti-apoptogenic and growth-stimulating actions on the splenocytes. It was demonstrated that IDO and NO restrained BCG-induced polarization and conversely COG-2 promoted BCG-induced pro-inflammatory polarization of MSC.Conclusions. 1. MSC actively participate in the formation of immunologic anti-mycobacterial resistance. 2. Targeted regulation of IDO and NO production can be feasibly applied for formation of anti-tuberculous vaccinal immunity and control mycobacterial infection.Β ΠΡΠΈ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Π² ΠΎΡΠ°Π³Π°Ρ
Π³ΡΠ°Π½ΡΠ»Π΅ΠΌΠ°ΡΠΎΠ·Π½ΠΎΠΉ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠΈΡΡΡ Π±ΠΎΠ»ΡΡΠΎΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ (ΠΠ‘Π), ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΊΠΎΡΠΎΡΡΡ
ΠΈ, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ, ΡΡΠ½ΠΊΡΠΈΡ Π² ΠΏΡΠΎΡΠΈΠ²ΠΎΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π½ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ΅ Π½Π΅ΠΈΠ·Π²Π΅ΡΡΠ½Ρ.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°ΡΡ ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΠ‘Π, ΡΠΎΡΠΌΠΈΡΡΡΡΠΈΠΉΡΡ ΠΏΡΠΈ ΠΈΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΈ Ρ M. bovis ΠΠ¦Π, ΠΈ ΠΎΡΠ΅Π½ΠΈΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΠΎΠ³ΠΎ ΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² ΠΈ ΡΡΠΈΠΌΡΠ»ΡΡΠΎΡΠΎΠ² ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΠ‘Π, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΈΠ· ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΠΌΡΡΠ΅ΠΉ, ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π»ΠΈ Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ ΠΈΠ»ΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ M. bovis ΠΠ¦Π ΠΈ/ΠΈΠ»ΠΈ Π°Π³ΠΎΠ½ΠΈΡΡΠ° TLR3 ΠΏΠΎΠ»ΡΠ΄Π°Π½Π° ΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΡ
Π½Π° ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΡ ΠΏΡΠΎ- ΠΈ Π°Π½ΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ². ΠΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΈ ΡΠ°Π΄ΠΈΠΎΠ°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΠΠ¦Π- ΠΈ ΠΏΠΎΠ»ΡΠ΄Π°Π½-ΠΊΠΎΠ½Π΄ΠΈΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΠ‘Π Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ ΠΈ Π°ΠΏΠΎΠΏΡΠΎΡΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΏΠ»Π΅Π½ΠΎΡΠΈΡΠΎΠ². Π ΡΠ°ΡΡΡ ΠΊΡΠ»ΡΡΡΡ Π²ΠΌΠ΅ΡΡΠ΅ Ρ ΠΠ¦Π ΠΈΠ»ΠΈ ΠΏΠΎΠ»ΡΠ΄Π°Π½ΠΎΠΌ Π΄ΠΎΠ±Π°Π²Π»ΡΠ»ΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ ΠΈΠ½Π΄ΠΎΠ»Π°ΠΌΠΈΠ½-2,3-Π΄ΠΈΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Ρ (ΠΠΠ), ΡΠΈΠΊΠ»ΠΎΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Ρ-2 (Π¦ΠΠ-2) ΠΈΠ»ΠΈ NO ΡΠΈΠ½ΡΠ°Π·Ρ ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π²ΠΊΠ»Π°Π΄ ΠΠΠ, Π¦ΠΠ-2 ΠΈ NO Π² ΠΠ¦Π- ΠΈ ΠΏΠΎΠ»ΡΠ΄Π°Π½-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ ΠΎΡΠ²Π΅Ρ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΏΠΎΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΠ‘Π ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΠΠ¦Π ΠΈ ΠΏΠΎΠ»ΡΠ΄Π°Π½Π°. ΠΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΠ‘Π ΠΊΠΎΡΡΠ΅Π»ΠΈΡΠΎΠ²Π°Π» Ρ ΠΈΡ
Π°Π½ΡΠΈΠ°ΠΏΠΎΠΏΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΈ ΡΠΎΡΡΠΎΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠ΅ΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΡΠΏΠ»Π΅Π½ΠΎΡΠΈΡΠ°ΠΌ. ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ, ΡΡΠΎ ΠΠΠ ΠΈ NO ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°ΡΡ ΠΠ¦Π-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΏΠΎΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ, Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π¦ΠΠ-2, Π½Π°ΠΎΠ±ΠΎΡΠΎΡ, ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΠ¦Π-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΠΠ‘Π.ΠΡΠ²ΠΎΠ΄Ρ. 1. ΠΠ‘Π ΡΠ²Π»ΡΡΡΡΡ Π°ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΡΡΠ°ΡΡΠ½ΠΈΠΊΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°Π½ΡΠΈΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ. 2. Π’Π°ΡΠ³Π΅ΡΠ½ΠΎΠ΅ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΠΠ ΠΈ ΠΎΠΊΡΠΈΠ΄Π° Π°Π·ΠΎΡΠ° ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎ Π΄Π»Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π½ΠΎΠ³ΠΎ Π²Π°ΠΊΡΠΈΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° ΠΈ Π΄Π»Ρ Π±ΠΎΡΡΠ±Ρ Ρ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ
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