2,003 research outputs found
Transport coefficients for electrolytes in arbitrarily shaped nano and micro-fluidic channels
We consider laminar flow of incompressible electrolytes in long, straight
channels driven by pressure and electro-osmosis. We use a Hilbert space
eigenfunction expansion to address the general problem of an arbitrary cross
section and obtain general results in linear-response theory for the hydraulic
and electrical transport coefficients which satisfy Onsager relations. In the
limit of non-overlapping Debye layers the transport coefficients are simply
expressed in terms of parameters of the electrolyte as well as the geometrical
correction factor for the Hagen-Poiseuille part of the problem. In particular,
we consider the limits of thin non-overlapping as well as strongly overlapping
Debye layers, respectively, and calculate the corrections to the hydraulic
resistance due to electro-hydrodynamic interactions.Comment: 13 pages including 4 figures and 1 table. Typos corrected. Accepted
for NJ
Mass and charge transport in micro and nano-fluidic channels
We consider laminar flow of incompressible electrolytes in long, straight
channels driven by pressure and electro-osmosis. We use a Hilbert space
eigenfunction expansion to address the general problem of an arbitrary cross
section and obtain general results in linear-response theory for the mass and
charge transport coefficients which satisfy Onsager relations. In the limit of
non-overlapping Debye layers the transport coefficients are simply expressed in
terms of parameters of the electrolyte as well as the hydraulic radius R=2A/P
with A and P being the cross-sectional area and perimeter, respectively. In
articular, we consider the limits of thin non-overlapping as well as strongly
overlapping Debye layers, respectively, and calculate the corrections to the
hydraulic resistance due to electro-hydrodynamic interactions.Comment: Invited paper presented at the Second International Conference on
Transport Phenomena in Micro and Nanodevices, Il Ciocco Hotel and Conference
Center, Barga, Italy, 11-15 June 2006. Accepted for publication in a special
issue of Nanoscale and Microscale Thermophysical Engineering (Taylor &
Francis
Structural and evolutionary relationships among protein tyrosine phosphatase domains
With the current access to the whole genomes of various organisms and the completion of the first draft of the human genome, there is a strong need for a structure-function classification of protein families as an initial step in moving from DNA databases to a comprehensive understanding of human biology. As a result of the explosion in nucleic acid sequence information and the concurrent development of methods for high-throughput functional characterization of gene products, the genomic revolution also promises to provide a new paradigm for drug discovery, enabling the identification of molecular drug targets in a significant number of human diseases. This molecular view of diseases has contributed to the importance of combining primary sequence data with three-dimensional structure and has increased the awareness of computational homology modeling and its potential to elucidate protein function. In particular, when important proteins or novel therapeutic targets are identified—like the family of protein tyrosine phosphatases (PTPs) (reviewed in reference 53)—a structure-function classification of such protein families becomes an invaluable framework for further advances in biomedical science. Here, we present a comparative analysis of the structural relationships among vertebrate PTP domains and provide a comprehensive resource for sequence analysis of phosphotyrosine-specific PTPs
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MicroRNA-Mediated mRNA Translation Activation in Quiescent Cells and Oocytes Involves Recruitment of a Nuclear microRNP
MicroRNAs can promote translation of specific mRNAs in quiescent (G0) mammalian cells and immature Xenopus laevis oocytes. We report that microRNA-mediated upregulation of target mRNAs in oocytes is dependent on nuclear entry of the microRNA; cytoplasmically-injected microRNA repress target mRNAs. Components of the activation microRNP, AGO, FXR1 (FXR1-iso-a) and miR16 are present in the nucleus and cytoplasm. Importantly, microRNA target mRNAs for upregulation, Myt1, TNFα and a reporter bearing the TNFα AU-rich, microRNA target sequence, are associated with AGO in immature oocyte nuclei and AGO2 in G0 human nuclei, respectively. mRNAs that are repressed or lack target sites are not associated with nuclear AGO. Crosslinking-coupled immunopurification revealed greater association of AGO2 with FXR1 in the nucleus compared to cytoplasm. Consistently, overexpression of FXR1-iso-a rescues activation of cytoplasmically-injected RNAs and in low density, proliferating cells. These data indicate the importance of a compartmentalized AGO2-FXR1-iso-a complex for selective recruitment for microRNA-mediated upregulation
Photo-antagonism of the GABAA receptor
Neurotransmitter receptor trafficking is fundamentally important for synaptic transmission and neural network activity. GABAA receptors and inhibitory synapses are vital components of brain function, yet much of our knowledge regarding receptor mobility and function at inhibitory synapses is derived indirectly from using recombinant receptors, antibody-tagged native receptors and pharmacological treatments. Here we describe the use of a set of research tools that can irreversibly bind to and affect the function of recombinant and neuronal GABAA receptors following ultraviolet photoactivation. These compounds are based on the competitive antagonist gabazine and incorporate a variety of photoactive groups. By using site-directed mutagenesis and ligand-docking studies, they reveal new areas of the GABA binding site at the interface between receptor β and α subunits. These compounds enable the selected inactivation of native GABAA receptor populations providing new insight into the function of inhibitory synapses and extrasynaptic receptors in controlling neuronal excitation
Long-Term Gynecological Outcomes in Women with Congenital Adrenal Hyperplasia due to 21-Hydroxylase Deficiency
Background. Our knowledge on long-term outcome in CAH remains incomplete.
Methods. In a prospective study (33 CAH patients, 33 age-matched controls), reproductive outcomes, self-rating of genital appearance and function, and sexuality were correlated to degree of initial virilisation, genotype, and surgery.
Results. Patients had larger median clitoral lengths (10.0 mm [range 2–30] versus 3.5 [2–8], P < .001), shorter vaginal length (121 mm [100–155] versus 128 [112–153], P = .12), lower uterine volumes (29.1 ml [7.5–56.7] versus 47.4 [15.9–177.5], P = .009), and higher ovarian volumes (4.4 ml [1.3–10.8] versus 2.8 [0.6–10.8], P = .09) than controls. Satisfaction with genital appearance was lower and negatively correlated to degree of initial virilisation (rs = ≤−0.39, P ≤ .05). More patients had never had intercourse (P = .001), and age at 1st intercourse was higher (18 yrs versus 16 yrs, P = .02).
Conclusion. Despite overall acceptable cosmetic results, reproductive outcomes were suboptimal, supporting that multidisciplinary teams should be involved in adult follow up of CAH patients
Angle dependence of Andreev scattering at semiconductor-superconductor interfaces
We study the angle dependence of the Andreev scattering at a
semiconductor-superconductor interface, generalizing the one-dimensional theory
of Blonder, Tinkham and Klapwijk. An increase of the momentum parallel to the
interface leads to suppression of the probability of Andreev reflection and
increase of the probability of normal reflection. We show that in the presence
of a Fermi velocity mismatch between the semiconductor and the superconductor
the angles of incidence and transmission are related according to the
well-known Snell's law in optics. As a consequence there is a critical angle of
incidence above which only normal reflection exists. For two and
three-dimensional interfaces a lower excess current compared to ballistic
transport with perpendicular incidence is found. Thus, the one-dimensional BTK
model overestimates the barrier strength for two and three-dimensional
interfaces.Comment: 8 pages including 3 figures (revised, 6 references added
Spontaneous emission from large quantum dots in nanostructures: exciton-photon interaction beyond the dipole approximation
We derive a rigorous theory of the interaction between photons and spatially
extended excitons confined in quantum dots in inhomogeneous photonic materials.
We show that, beyond the dipole approximation, the radiative decay rate is
proportional to a non-local interaction function, which describes the
interaction between light and spatially extended excitons. In this regime,
light and matter degrees of freedom cannot be separated and a complex interplay
between the nanostructured optical environment and the exciton envelope
function emerges. We illustrate this by specific examples and derive a series
of important analytical relations, which are useful for applying the formalism
to practical problems. In the dipole limit, the decay rate is proportional to
the projected local density of optical states and we obtain the strong and weak
confinement regimes as special cases.Comment: 14 pages, 4 figure
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