Semiotics of Vaccination Adver-tising in Media Discourse

The aim of the article is to analyze the verbal and visual means in advertising COVID-19 vaccination. The task is to examine the peculiarities of semiotics in vaccination advertising based on campaigns from different countries around the world, identify successful advertising strategies, and discover the reasons for communicative failures. The study analyzes the techniques of combining visual and verbal narratives in creolized advertising texts. It emphasizes that it is the visual narrative that appeals to the subconscious, which often contributes to the recipient’s necessary post-communicative action. The article examines the tactics of argumentation used in vaccination advertising (including appeals to authority, values such as health, love, freedom, etc.), studies the semiotics of the visual elements involved in argumentation. The article also analyzes the semiotics of color signals in advertising discourse, revealing their influence on the recipient’s subconscious. Examples of using semantic signs taken from other semantic domains, such as election campaigns and road sign systems, are separately considered in vaccination campaigns. The creativity of these techniques is noted, but the danger of visual disconnect with slogans and communicative failure is also indicated. The conclusion is drawn that advertising appealing to positive feelings of social responsibility, comfort, and well-being is more effective than advertising using threat-based argumentation

Purpuromycin: an antibiotic inhibiting tRNA aminoacylation.

Purpuromycin, an antibiotic produced by Actinoplanes ianthinogenes, had been reported previously to inhibit protein synthesis. In the present report, we demonstrate that the mechanism of action of this antibiotic is quite novel in that it binds with fairly high affinity to all tRNAs, inhibiting their acceptor capacity. Although more than one molecule of purpuromycin is bound to each tRNA molecule, the inhibitory activity of this antibiotic was found to be selective for the tRNA acceptor function; in fact, after the aminoacylation step, purpuromycin was found to affect none of the other tested functions of tRNA (interaction with the ribosomal P- and A-sites and interaction with translation factors). Accordingly, purpuromycin was found to inhibit protein synthesis only when translation depended on the aminoacylation of tRNA and not when the system was supplemented with pre-formed aminoacyl-tRNAs. Because purpuromycin did not interfere with the ATP-PPi exchange reaction of the synthetase or with the initial interaction of the enzyme with its tRNA substrate, the basis for the inhibition of aminoacylation is presumably the formation of a nonproductive synthetase-tRNA complex in the presence of purpuromycin in which the tRNA is unable to be charged with the corresponding amino acid

ORGANIZING COMMITEE

Присвячена 60-рiччю кафедри теорiї ймовiрностей i математичної статистики та пам’ятi М.Й.Ядренка (1932-2004) МАТЕРIАЛИ КОНФЕРЕНЦIЇ 19-23 червня, 200