Assessment of the genetic diversity of African yam bean (Sphenostylis stenocarpa Hochst ex. A Rich. Harms) accessions using amplified fragment length polymorphism (AFLP) markers

The genetic diversity of 40 African yam bean (AYB) accessions was assessed using amplified fragment length polymorphism (AFLP) markers. Seeds of 40 accessions of AYB obtained from the International Institute of Tropical Agriculture (IITA) and Institute of Agricultural Research and Training (IAR&T) Ibadan, Nigeria, were grown in a greenhouse and young leaves from two weeks old plants collected for DNA extraction. The four primer combinations used generated a total of 1730 amplification fragments across the AYB accessions used in this study of which 1647 were polymorphic (95.20%). The number of amplified polymorphic AFLP bands per primer pair varied from 360 to 520 with an average percentage polymorphism of 95.6%. E-AGC/M-CAG produced the highest number of polymorphic bands (520). Polymorphic information content (PIC) values ranged from 0.9447 to 0.9626. The highest level of polymorphism (100%) was recorded for two primer combinations (E-AAC/M-CAG and E-ACT/M-CAG). The results of cluster analysis using UPGMA tree, grouped the 40 accessions of AYB into two major clusters with an overall similarity of 67.5%. The level of similarity between the accessions spanned 0.66 to 0.91. TSs 138 and TSs 139 were the most closely related accessions with high level of similarity index (0.91). Comparable results were obtained using Factorial Coordinate Analysis (FCO).  The results from the present study confirm the robustness and the suitability of the AFLP as a molecular tool for the assessment of genetic diversity in AYB accessions.Keywords: Amplified fragment length polymorphism (AFLP), cluster analysis, genetic diversity, Sphenostylis stenocarpa, polymorphismAfrican Journal of Biotechnology< Vol 13(18), 1850-185

The exploitation of orphan legumes for food, income, and nutrition security in sub-Saharan Africa

Open Access Journal; Published online: 17 May 2022Poverty, food, and nutrition insecurity in sub-Saharan Africa (SSA) have become major concerns in recent times. The effects of climate change, drought, and unpredictable rainfall patterns threaten food production and sustainable agriculture. More so, insurgency, youth restiveness, and politico-economic instability amidst a burgeoning population requiring a sufficient and healthy diet remain front-burner issues in the region. Overdependence on only a few major staple crops is increasingly promoting the near extinction of many crops, especially orphan legumes, which possess immense potentials as protein and nutritional security crops. The major staple crops are declining in yield partly to their inability to adapt to the continuously changing climatic conditions. Remarkably, the orphan legumes are climate-smart crops with enormous agronomic features which foster sustainable livelihood. Research efforts on these crops have not attained a reasonable comparative status with most commercial crops. Though many research organizations and scientists have made efforts to promote the improvement and utilization of these orphan legumes, there is still more to be done. These legumes’ vast genetic resources and economic utility are grossly under-exploited, but their values and promising impacts are immeasurable. Given the United Nations sustainable development goals (SDGs) of zero hunger, improved nutrition, health, and sustainable agriculture, the need to introduce these crops into food systems in SSA and other poverty-prone regions of the world is now more compelling than ever. This review unveils inherent values in orphan legumes needing focus for exploitation viz-a-viz cultivation, commercialization, and social acceptance. More so, this article discusses some of the nutraceutical potentials of the orphan legumes, their global adaptability, and modern plant breeding strategies that could be deployed to develop superior phenotypes to enrich the landraces. Advanced omics technologies, speed breeding, as well as the application of genome editing techniques, could significantly enhance the genetic improvement of these useful but underutilized legumes. Efforts made in this regard and the challenges of these approaches were also discussed

Morphometric analysis and characterization of the nutritional quality in African yam bean accessions

Underutilized crops with rich nutritional base in addition to high seed yield are currently promoted in reducing hunger and malnutrition ravaging Africans. Identifying specific accessions with rich genetic bases provide information for developing biofortified legumes. Fourteen morphological and two nutritional traits of 23 African yam bean accessions were assessed under a replicated field trial in one location over 4 years. Analysis of variance revealed significant variations for most traits with high genotypic correlation coefficients. Genotypic coefficient of variation was high for maturity date (59.95%), protein content (60.78%), tuber yield per plant (67.33%) and number of seeds per pod (77.22%). Seed yield did not correlate with protein and oil content, indicating that nutritional traits were independent of seed yield traits. Heritability was high for most traits but moderate for protein and oil content. First order predictor variables, identified number of seeds per pod, and pod traits to directly affect seed and tuber yield. First seven principal components axes explained 67% of total variation including nutritional traits. Four distinct accession clusters at the R-squared distance of 0.45 similarity index were identified. Simultaneous improvement in earliness, yield, protein and oil contents are achievable by selecting parents from clusters I and II. The only accession in cluster III (TSs12) produced nodules in addition to average seed yield, protein and oil content, and could be selected for cultivation in nitrogen deficient soils. Wide diversity in these accessions can be maximized for developing acceptable African yam bean varieties with enhanced yield and nutritional value

The Exploitation of Orphan Legumes for Food, Income, and Nutrition Security in Sub-Saharan Africa

Poverty, food, and nutrition insecurity in sub-Saharan Africa (SSA) have become major concerns in recent times. The effects of climate change, drought, and unpredictable rainfall patterns threaten food production and sustainable agriculture. More so, insurgency, youth restiveness, and politico-economic instability amidst a burgeoning population requiring a sufficient and healthy diet remain front-burner issues in the region. Overdependence on only a few major staple crops is increasingly promoting the near extinction of many crops, especially orphan legumes, which possess immense potentials as protein and nutritional security crops. The major staple crops are declining in yield partly to their inability to adapt to the continuously changing climatic conditions. Remarkably, the orphan legumes are climate-smart crops with enormous agronomic features which foster sustainable livelihood. Research efforts on these crops have not attained a reasonable comparative status with most commercial crops. Though many research organizations and scientists have made efforts to promote the improvement and utilization of these orphan legumes, there is still more to be done. These legumes’ vast genetic resources and economic utility are grossly under-exploited, but their values and promising impacts are immeasurable. Given the United Nations sustainable development goals (SDGs) of zero hunger, improved nutrition, health, and sustainable agriculture, the need to introduce these crops into food systems in SSA and other poverty-prone regions of the world is now more compelling than ever. This review unveils inherent values in orphan legumes needing focus for exploitation viz-a-viz cultivation, commercialization, and social acceptance. More so, this article discusses some of the nutraceutical potentials of the orphan legumes, their global adaptability, and modern plant breeding strategies that could be deployed to develop superior phenotypes to enrich the landraces. Advanced omics technologies, speed breeding, as well as the application of genome editing techniques, could significantly enhance the genetic improvement of these useful but underutilized legumes. Efforts made in this regard and the challenges of these approaches were also discussed