Comparative Fatty Acid Profiling of Klebsiella pneumoniae and Rhodococcus rhodochrous Isolated from Spoilt Paints by Gas Chromatography

The use of fatty acids to study the differences in un-related microbes is limited. This study analyzes the fatty acids produced by two unrelated microorganisms: Klebsiella pneumoniae (Gram-negative, aerobic, non-endospore forming, usually encapsulated rod-shaped bacteria of the family Enterobacteriaceae) and Rhodococcus rhodochrous (metabolically versatile, non-spore-forming, non-motile actinomycete) isolated from spoilt paints. Fatty acids produced by the organisms were analyzed using an efficient MIDI-Sherlock gas chromatography method . K. pneumoniae was characterized by a high content of straight chain, branched chain, hydroxyl and cyclo-fatty acids made up of C12: 0, C13:0, C14:0 iso, C14:0, C15:0 iso, C15:0 anteiso, C15:1 ω 8c, C15:0, C16:0 iso, C16:1w5c, C16:0, C15:03OH, C17:1 ω 8c, C17:0 cyclo, C17:0, C18:1 ω5c and C18:0. R. rhodochrous was dominated by straight chain, monounsaturated and 10-methyl fatty acids. The inability to synthesize branched, cyclo- and hydroxyl- fatty acids, was observed in R. rhodochrous which composed mainly of C14: 0, C15: 1 ω 5c, C15:0, C16:1 ω 9c, C16:0, C17:1 ω 8c, C17:0, C17:0 10-methyl, C18: 1 ω 9c, C18.0, 10 methyl-C18:0 TBSA, C20:1 ω 9c, and C20:0. Descriptive statistics reveal a mean of 2.53, 15.10 and 15.15 for retention time (RT), equivalent chain length (ECL) and Peak name, respectively. Possible implications of the variations in fatty acid distribution may include differences in their abilities to produce various secondary metabolites and potentials to degrade a variety of xenobiotics. Keywords: Fatty acids, paints, Rhodococcus rhodochrous, Klebsiella pneumonia

Occurrence and recalcitrance of polyethylene bag waste in Nigerian soils

Attempts to biodegrade polyethylene bag wastes (pure water sachets) weighing 25.2 g each were made by burying them in the soil and subjecting them to acid treatment (0.5 M HNO3) and alkaline treatment (0.5 M NaOH) over a 24 week study period. The experimental polyethylene bags (ED1) were inoculated with a strain of Pseudomonas spp. isolated from a refuse dump and by inorganic nutrient supplementation to facilitate biodegradation. Samples without inorganic nutrient supplementation and Pseudomonas spp. inoculation served as first control (CD1), while those placed on a slab on the surface of the soil served as second control (CD2) to evaluate the roles played by microorganisms and physical degradation, respectively. ED1 showed a slight reduction in weight to 24.9 g at week 16 after the acid treatment for 5 days and further reduced to 24.7 g at the end of the study after the alkaline treatment. CD1 reflected no variations in weight, while CD2 reduced to 25.1 g. The study showed that recycling of used pure water sachets which yielded useful products such as water seal, polyethylene bags and jerry can covers, appears to be the only option of checking environmental pollution caused by this product as microbial degradation proved ineffective.Key words: Biodegradation, polyethylene, waste, recycling, recalcitrance

Molecular Characterization of Successive Yeasts Strains and their Optimal Invertase Producing Conditions in Fresh Palm Wine (Raphiahookeri) Obtained from Lagos, Nigeria

Saccharomyces cerevisiae is known to produce invertase. However, molecular characterization of successive yeast strains and their optimal invertase producing conditions in Nigerian Raphiahookeri has not been documented. In this study, molecular characterization of successive yeast strains and their optimal invertase producing conditions in fresh palm wine (Raphiahookeri) obtained from Lagos, Nigeria was evaluated. To achieve this, sequencing of 18S rDNA was carried out following DNA extraction from the yeast isolates. Invertase production within 5-day period was also monitored at different conditions for the yeast strains. The physicochemical parameters investigated include pH, optical density, titratable acidity, sugar concentration and alcohol content. Out of the 21isolates obtained, 18 were identified by 18S rDNA to beS. cerevisiae, while 3 were Penicillium chrysogenum, Blumeriagraminissp. Tritici and Fusarium culmorum. Thefungal population density decreased from 1.01 x 107 to 0.9 x 105CFU/ml. The pH and sugar concentration decreased with increase in age of the wine ranging from 6.05-3.33 and 3.70-1.10mg/100ml respectively. The optical density, titratable acidity and alcohol content increased with time and ranged from 0.454-0.904, 0.266-0.412 % and 0.920-5.640 % respectively. All isolates produced invertase optimally at pH 6, 30°C after 48 h incubation with S. cerevisiae CP006433.1 having the highest production (1.745U/ml).The results highlight the effect of fermentation conditions on the survival and invertase production of yeast strains in Nigerian palm wine

Dataset for metagenomic analysis of microbial community of spoilt paints

Data describes the various microorganisms in fresh and spoilt paint samples through culture-based analysis using nutrient agar and centrimide agar. Secondly, it describes a richer diversity of microorganisms in the spoilt paint samples through metagenomic analysis. It further describes the variation in the chemical components of the two type of samples using GC-M

Generating Heterogeneous Big Data Set for Healthcare and Telemedicine Research Based on ECG, Spo2, Blood Pressure Sensors, and Text Inputs: Data set classified, Analyzed, Organized, And Presented in Excel File Format.

Heterogenous Big dataset is presented in this proposed work: electrocardiogram (ECG) signal, blood pressure signal, oxygen saturation (SpO2) signal, and the text input. This work is an extension version for our relevant formulating of dataset that presented in [1] and a trustworthy and relevant medical dataset library (PhysioNet [2]) was used to acquire these signals. The dataset includes medical features from heterogenous sources (sensory data and non-sensory). Firstly, ECG sensor’s signals which contains QRS width, ST elevation, peak numbers, and cycle interval. Secondly: SpO2 level from SpO2 sensor’s signals. Third, blood pressure sensors’ signals which contain high (systolic) and low (diastolic) values and finally text input which consider non-sensory data. The text inputs were formulated based on doctors diagnosing procedures for heart chronic diseases. Python software environment was used, and the simulated big data is presented along with analyses

Generating Heterogeneous Big Data Set for Healthcare and Telemedicine Research Based on ECG, Spo2, Blood Pressure Sensors, and Text Inputs: Data set classified, Analyzed, Organized, And Presented in Excel File Format.

Heterogenous Big dataset is presented in this proposed work: electrocardiogram (ECG) signal, blood pressure signal, oxygen saturation (SpO2) signal, and the text input. This work is an extension version for our relevant formulating of dataset that presented in [1] and a trustworthy and relevant medical dataset library (PhysioNet [2]) was used to acquire these signals. The dataset includes medical features from heterogenous sources (sensory data and non-sensory). Firstly, ECG sensor’s signals which contains QRS width, ST elevation, peak numbers, and cycle interval. Secondly: SpO2 level from SpO2 sensor’s signals. Third, blood pressure sensors’ signals which contain high (systolic) and low (diastolic) values and finally text input which consider non-sensory data. The text inputs were formulated based on doctors diagnosing procedures for heart chronic diseases. Python software environment was used, and the simulated big data is presented along with analyses.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Vibrational spectroscopy and non-parametric analysis for biodegradation evaluation: The acrylic-emulsion paint scenario

In this work, we utilized Raman spectroscopy, characterized by excellent lateral resolution, in capturing the degradation of paints was investigated. Six fresh and six spoilt acrylic emulsion paint samples obtained from a paint company in Lagos, Nigeria and were used for this study. They were analyzed for microbial population and types using total viable count method and analytical profile index (API20E &20NE). Physicochemical quality markers [transmittance (TR), pH, OD600, and specific gravity (SG)] of the paints were evaluated by standard methods. Raman spectroscopy was deployed to analyze components of both fresh and spoilt paint samples. Lead (Pb) concentrations were assessed using atomic absorption spectrophotometry.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Dataset for metagenomic analysis of microbial community of spoilt paints

Data describes the various microorganisms in fresh and spoilt paint samples through culture-based analysis using nutrient agar and centrimide agar. Secondly, it describes a richer diversity of microorganisms in the spoilt paint samples through metagenomic analysis. It further describes the variation in the chemical components of the two type of samples using GC-M

Evaluating the impact of TiO2nanoparticles with varying capping agents on cell integrity of Myroides odoratus isolated from spoilt paint coating

The impact of okro (O), basil (B) and spearmint (S) capped TiO2NPs on cell integrity of Myroides odoratus MZ442205 isolated from spoilt paint was investigated. TiO2NPs were synthesized through green routes. The antibacterial activity of the NPs at 5, 10, 20, 40 and 80 mg/ml was evaluated using diffusion technique. Damage on bacterium cell wall after NPs treatment was investigated using differential plating method, UV-Vis and SEM. The Zone of inhibition ranged from 2-5, 2-6, and1-3.5 mm; MIC was 20, 40 and 40 mg/ml while MBC was >80mg/ml for O, B and S capped TiO2NPs respectively. UV-Vis showed shifts in NPs compared to the bulk with absorptions at 211nm and 265nm respectively. Treated cells had 19-26% increase in absorbance at 260nm with increasing NPs concentration indicating leakages of nucleic acid materials into medium compared with the control without NPs. This was corroborated by reduced viable cell population in selective blood agar medium (8.15 x 104, 7.65 x 104 and 0.82 x 104CFU/mL) compared to general purpose nutrient broth (9.3 x 104, 8.7 x 104 and 0.88 x 104 CFU/mL) at 80mg/ml respectively. SEM showed spherical NPs, treated cells with cell wall pitting suggesting susceptibility to TiO2NPs via compromised cell integrity.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Evaluating the impact of TiO2nanoparticles with varying capping agents on cell integrity of Myroides odoratus isolated from spoilt paint coating

The impact of okro (O), basil (B) and spearmint (S) capped TiO2NPs on cell integrity of Myroides odoratus MZ442205 isolated from spoilt paint was investigated. TiO2NPs were synthesized through green routes. The antibacterial activity of the NPs at 5, 10, 20, 40 and 80 mg/ml was evaluated using diffusion technique. Damage on bacterium cell wall after NPs treatment was investigated using differential plating method, UV-Vis and SEM. The Zone of inhibition ranged from 2-5, 2-6, and1-3.5 mm; MIC was 20, 40 and 40 mg/ml while MBC was >80mg/ml for O, B and S capped TiO2NPs respectively. UV-Vis showed shifts in NPs compared to the bulk with absorptions at 211nm and 265nm respectively. Treated cells had 19-26% increase in absorbance at 260nm with increasing NPs concentration indicating leakages of nucleic acid materials into medium compared with the control without NPs. This was corroborated by reduced viable cell population in selective blood agar medium (8.15 x 104, 7.65 x 104 and 0.82 x 104CFU/mL) compared to general purpose nutrient broth (9.3 x 104, 8.7 x 104 and 0.88 x 104 CFU/mL) at 80mg/ml respectively. SEM showed spherical NPs, treated cells with cell wall pitting suggesting susceptibility to TiO2NPs via compromised cell integrity.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV