"Enfezamento pálido" e "enfezamento vermelho" na cultura do milho no Brasil Central.

Foi constatada alta incidencia de plantas com sintomas de "Enfezamento Palido" e de "Enfezamento vermelho" em lavouras de milho no Brasil Central. Na mesma area observou-se a presenca de cigarrinha Dalbulus maidis em alta densidade. A presenca de espiroplasma foi confirmada apenas em plantas com sintomas de "Enfezamento Palido", atraves de teste DAS-ELISA. Os resultados da analise por PCR indicaram a presenca de fitoplasma em algumas amostras de plantas com sintomas do "enfezamento Vermelho"

Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the genus Tospovirus.

Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history

Extraction of a strongly focusing He + beam from three-stage concave electrodes for alpha particle measurement system in ITER

A strongly focusing He + ion beam source equipped with concave multi-aperture electrodes was developed for production of He - through a charge exchange cell. The beam was extracted at a voltage less than 20 kV from 301 apertures distributed in an area of 100 mm , and focused at 750 mm distance. The beam current and the beam size of 2 A and 20 mm in diameter, respectively, were achieved with an arc power less than 10 kW. The optimum perveance was obtained at 0.02 AkV 1.5 at the beam energy less than 20 keV which is suitable for the conversion to He - in an alkali vapor cell

Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil.

A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR