Opisthorchis viverrini infection induces metabolic and fecal microbial disturbances in association with liver and kidney pathologies in hamsters

All spectral data obtained from urine, hepatic and serum hamsters. The hamsters were allocated into two groups with 20 per group: 1) Ov+, the infected group, which hamsters were orally inoculated 50 metacercariae of O. viverrini; and 2) Ov-, non-infected control group at 1 and 4 mpi

Opisthorchis viverrini infection induces metabolic and fecal bacterial composition disturbances in hamsters fed with high fat/high fructose diets: an implication in liver and kidney pathologies

The raw gut bacterial sequencing data obtained from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

Opisthorchis viverrini infection induces metabolic and fecal microbial disturbances in association with liver and kidney pathologies in hamsters

All spectral data obtained from urine, hepatic and serum hamsters. The hamsters were allocated into two groups with 20 per group: 1) Ov+, the infected group, which hamsters were orally inoculated 50 metacercariae of O. viverrini; and 2) Ov-, non-infected control group at 1 and 4 mpi

Opisthorchis viverrini infection induces metabolic and fecal bacterial composition disturbances in hamsters fed with high fat/high fructose diets: an implication in liver and kidney pathologies

The NMR spectral data from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

METABOLIC PROFILING IN OPISTHORCHIASIS AND IMPACT OF HIGH FAT AND HIGH FRUCTOSE DIETS ON OPISTHORCHIASIS IN HAMSTERS

The NMR spectral data from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

METABOLIC PROFILING IN OPISTHORCHIASIS AND IMPACT OF HIGH FAT AND HIGH FRUCTOSE DIETS ON OPISTHORCHIASIS IN HAMSTERS

The NMR spectral data from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

Opisthorchis viverrini infection induces metabolic and fecal microbial disturbances in association with liver and kidney pathologies in hamsters

All spectral data obtained from urine, hepatic and serum hamsters. The hamsters were allocated into two groups with 20 per group: 1) Ov+, the infected group, which hamsters were orally inoculated 50 metacercariae of O. viverrini; and 2) Ov-, non-infected control group at 1 and 4 mpi

Opisthorchis viverrini infection induces metabolic and fecal bacterial composition disturbances in hamsters fed with high fat/high fructose diets: an implication in liver and kidney pathologies

The raw gut bacterial sequencing data obtained from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

Opisthorchis viverrini infection induces metabolic and fecal bacterial composition disturbances in hamsters fed with high fat/high fructose diets: an implication in liver and kidney pathologies

The NMR spectral data from 4 groups of hamsters:1. non-infected hamsters and fed with standard chow (NC), 2. O. viverrini-infected hamster and fed with standard chow (OV), 3. non-infected hamsters fed with HFa/HFr diets (HF), and 4.O. viverrini-infected hamsters fed with HFa/HFr diets (OH) for 8 months

Plasma IgG autoantibody against actin-related protein 3 in liver fluke Opisthorchis viverrini infection

Opisthorchiasis secondary to Opisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. It was hypothesized that plasma autoantibodies directed against self-proteins are biomarkers for opisthorchiasis. Plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to LC-MS/MS. Seven protein spots were recognized by IgG autoantibodies, and the highest matching scored protein was actin-related protein 3 (ARP3). The antibody against ARP3 was tested in plasma from 55 O.\ua0viverrini-infected patients, 24 patients with others endemic parasitic infections and 17 healthy controls using Western blot and ELISA. Immunoreactivity against recombinant ARP3 was significantly more prevalent in opisthorchiasis compared to healthy controls at Western blotting and ELISA (P\ua0<\ua00\ub705). Plasma ARP3 autoantibody titres were also higher in opisthorchiasis compared to healthy individuals (P\ua0<\ua00\ub701) and other parasitic infections including Strongyloides stercoralis (P\ua0<\ua00\ub7001), echinostome (P\ua0<\ua00\ub705), hookworms (P\ua0< 0\ub7001) and Taenia spp. (P\ua0<\ua00\ub705). It was further characterized in that the ARP3 autoantibody titre had a sensitivity of 78\ub718% and specificity of 100% for opisthorchiasis. In conclusion, it may be suggested that plasma anti-ARP3 might represent a new diagnostic antibody for opisthorchiasis