Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-5

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>× 10cells/well and allowed to attach overnight. Cells were incubated with LM-234ep CM (black circles), LM-234mf CM (black triangles), or culture medium (black squares) containing 10% FBS (Control), and counted at different time points. Data are shown as mean ± SE. * = 0.05, ** = 0.0018, *** = 0.0006, **** < 0.0001 (Student's t Test). Assays were performed in triplicate

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-4

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p> and allowed to attach overnight. Thereafter, cells were incubated in the presence of LM-234ep CM (black circles), LM-234mf CM (black triangles), or culture medium (black squares) supplemented with 10% FBS. Cell numbers were counted at different time points, and expressed as mean ± SE. * = 0.05, ** = 0.0018 (Student's t Test). Assays were performed in triplicate, (A). LM-234mf cells were incubated with culture medium + 10% FBS (Control) or LM-234ep CM for 72 h. Cells were processed for DNA content, and cell cycle progression was analyzed by flow cytometry (B). LM-234mf cells were treated with culture medium (Control) or LM-234ep CM for the times indicated (top). Cells were collected, lysed, and analyzed by immunoblotting using antibodies specific for p-ERK, c-Jun, JunB, cyclins E, A, and D, and Cdk2. β-actin was used as a loading control (C). Immunofluorescence for c-Jun, Cyclin A and Cyclin D in LM-234mf cells. The nuclear localization of the proteins is shown in the control cells (white arrows). Magnification bar, 40 μm (D). AP-1 luciferase activity in LM-234mf cells co-transfected with AP-1-Luc and pRL-tk-luc and treated with LM-234ep CM or culture medium (Control). ** = 0.005 (Student's t Test). The results shown are the mean ± S.D. of three experiments (E)

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-7

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>ture medium (black squares), whereas tumors in the left flank were injected with LM-234ep CM (black triangles). Tumors were measured and volumes were calculated as mean ± SE. * = 0.018, ** = 0.007, *** = 0.004 (Student's t Test). Representative picture of a mouse showing inhibition of HeLa tumor growth by LM-234ep CM (arrow) as compared to culture medium treatment (contralateral flank), 4 weeks after tumor inoculation (C)

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-8

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>C-conjugated primary antibody. Nuclei (blue staining) were identified using DAPI. Cytokeratin was undetectable in LM-234mf (B), whereas strongly expressed in LM-234ep (D), as revealed by immunohistochemistry using an anti-Pan cytokeratin antibody. Magnification bar, 100 μm. Cell lysates (25 μg/lane) were analyzed using Western blot for cytokeratins (E) and αSMA (F). The cytokeratins detected with the anti-pan antibody used range between 40 and 68 kDa. The band immunodetected for αSMA has an approximate molecular weight of 42 kDa. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was used as a loading control

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-1

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>. A minimum of 50 metaphase spreads was analyzed in each case. Representative metaphases of LM-234ep (C) and LM-234mf (D) cells. Arrows point different chromosomal aberrations including: acentric fragments (1), chromosome break (2), and centromeric fusion (3). Magnification, × 1000

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-0

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>C-conjugated primary antibody. Nuclei (blue staining) were identified using DAPI. Cytokeratin was undetectable in LM-234mf (B), whereas strongly expressed in LM-234ep (D), as revealed by immunohistochemistry using an anti-Pan cytokeratin antibody. Magnification bar, 100 μm. Cell lysates (25 μg/lane) were analyzed using Western blot for cytokeratins (E) and αSMA (F). The cytokeratins detected with the anti-pan antibody used range between 40 and 68 kDa. The band immunodetected for αSMA has an approximate molecular weight of 42 kDa. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was used as a loading control

Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-2

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>hy, using 7F2 and BMA3.1A7 cell lines as positive controls for mouse MMP-2 and MMP-9, respectively (A). LM-234mf and ep cells were compared for their ability to invade through Matrigel-coated Transwell 8 μm-pore filters (B). The number of cells traversing the membrane was quantified and expressed as mean ± SE (n = 3)