<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p> and allowed to attach overnight. Thereafter, cells were incubated in the presence of LM-234ep CM (black circles), LM-234mf CM (black triangles), or culture medium (black squares) supplemented with 10% FBS. Cell numbers were counted at different time points, and expressed as mean ± SE. * = 0.05, ** = 0.0018 (Student's t Test). Assays were performed in triplicate, (A). LM-234mf cells were incubated with culture medium + 10% FBS (Control) or LM-234ep CM for 72 h. Cells were processed for DNA content, and cell cycle progression was analyzed by flow cytometry (B). LM-234mf cells were treated with culture medium (Control) or LM-234ep CM for the times indicated (top). Cells were collected, lysed, and analyzed by immunoblotting using antibodies specific for p-ERK, c-Jun, JunB, cyclins E, A, and D, and Cdk2. β-actin was used as a loading control (C). Immunofluorescence for c-Jun, Cyclin A and Cyclin D in LM-234mf cells. The nuclear localization of the proteins is shown in the control cells (white arrows). Magnification bar, 40 μm (D). AP-1 luciferase activity in LM-234mf cells co-transfected with AP-1-Luc and pRL-tk-luc and treated with LM-234ep CM or culture medium (Control). ** = 0.005 (Student's t Test). The results shown are the mean ± S.D. of three experiments (E)
