Determinación de serogrupos circulantes de Dichelobacter nodosusen majadas con diagnóstico clínico de Foot-Rot en ovinos de Uruguay. Resultados Preliminares. [abstract].

Las afecciones podales representan uno de los problemas de salud más importantes de los ovinos. Los animales afectados pierden bienestar y productividad. La principal enfermedad podal es el Foot-rot (Pietín); enfermedad infecto-contagiosa causada por Dichelobacter nodosus. En Uruguay existe escasa información actualizada respecto a cuáles son los serogrupos actuantes de D. nodosus y esto es muy importante para estrategias de control de la enfermedad a través de vacunaciones serogrupo-específicas

Virulence-associated subtilisin-like proteases that use a novel disulphide-tethered exosite to mediate substrate specificity (3LPA, 3LPC, 3LPD)

<div>Many bacterial pathogens produce extracellular proteases that are involved in the degradation of the host extracellular matrix. Dichelobacter nodosus, which causes ovine footrot, is one such pathogen, Mutagenesis and virulence studies revealed that AprV2, one of three secreted subtilisin-like D. nodosus proteases, is required for virulence. Our work challenges the previous hypothesis that the elastase activity of AprV2 is important for disease progression, since aprV2 mutants were virulent when complemented with a variant with impaired elastase activity. These data reveal that an unusual extended disulphide-tethered loop functions as an exosite that governs the ability of AprV2 to degrade insoluble extracellular matrix components. The disulphide bond and Tyr92, located at the exposed end of the loop, were functionally important. Bioinformatics suggests that other pathogens utilize a similar mechanism, providing a new paradigm for understanding the role of proteases in disease.</div><div><br></div><p></p