Low-Frequency Spectral Energy Distributions of Radio Pulsars Detected with the Murchison Widefield Array

We present low-frequency spectral energy distributions of 60 known radio pulsars observed with the Murchison Widefield Array telescope. We searched the GaLactic and Extragalactic All-sky Murchison Widefield Array survey images for 200-MHz continuum radio emission at the position of all pulsars in the Australia Telescope National Facility (ATNF) pulsar catalogue. For the 60 confirmed detections, we have measured flux densities in 20 × 8 MHz bands between 72 and 231 MHz. We compare our results to existing measurements and show that the Murchison Widefield Array flux densities are in good agreement

Correlation results between regional mean ALFF and neurocognitive scores.

<p>Correlation results between regional mean ALFF and neurocognitive scores.</p

Representative one-sample <i>t</i>-test result of ALFF maps.

<p>(A) CTL subjects. (B) ACTL subjects. (C) SCD subjects. Color scale indicates t-values, resulting from one one sample t-test. Thresholds were set at a AlphaSim corrected p<0.05, determined by Monte Carlo simulation. In the Montreal Neurological Institute (MNI) template, the planes are X = -4mm, Z = -32mm and Y = -52mm for the sagittal, axial and coronal views, respectively.</p

Demographic and clinical variables among healthy controls (CTL), anemic control subjects (ACTL) and patients with sickle cell disease (SCD) including the transfused (NTx) and the non-transfused patients.

<p>Demographic and clinical variables among healthy controls (CTL), anemic control subjects (ACTL) and patients with sickle cell disease (SCD) including the transfused (NTx) and the non-transfused patients.</p

Differences in ALFF values between ACTL, SCD and CTL groups.

<p>All thresholds were set at a AlphaSim corrected p<0.05. Color scale indicates t-values, resulting from two sample t-test. Numbers and arrows represent anatomical location. Numbers and arrows represent anatomical location. A. Representative two-sample <i>t</i>-test results of ALFF maps between the ACTL versus CTL groups showing the right mSFG (arrow 1) and left insula (arrow 2). B. Representative two-sample <i>t</i>-test results of ALFF maps between the SCD versus CTL groups. Seven clusters are indicated by arrows: OFC (arrow 3), right paracentral lobule (arrow 4), PCC (arrow 5), cerebellum (arrow 6), frontal pole (arrow 7), left precuneus (arrow 8) and left insula (arrow 2). C. Representative two-sample <i>t</i>-test results of ALFF maps between the SCD versus ACTL groups. Compared with values in the ACTL group, ALFF in SCD patients increased in OFC (3) and decreased in right mSFG (arrow 1), cerebellum (arrow 6) and frontal pole (arrow 7).</p

Venn diagram representing changes (from normal) seen in SCD and ACTL groups.

<p>The changes are grouped with regards to their cause. They could be either unique to SCD patients (left/grey), common to SCD and ACTL patients (middle) or unique to ACTL patients (right/blue). OFC: orbital frontal cortex; ACC: anterior cingulate cortex; PCC: posterior cingulate cortex; mSFG: medial superior frontal gyrus.</p

Cerebral volumes (expressed as mean ± standard deviation) of healthy controls (CTL), anemic control subjects (ACTL) and patients with sickle cell disease (SCD) and the p-values of SCD vs. CTL.

<p>Cerebral volumes (expressed as mean ± standard deviation) of healthy controls (CTL), anemic control subjects (ACTL) and patients with sickle cell disease (SCD) and the p-values of SCD vs. CTL.</p

Statistic results between the SCD patients with and without WMHs.

<p>Statistic results between the SCD patients with and without WMHs.</p

CRISPR off-target analysis in genetically engineered rats and mice

Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were confirmed, and show that high-fidelity Cas9 versions reduced off-target mutation rates in vivo. Using whole-genome sequencing data from ten mouse embryos, treated with a single guide RNA (sgRNA), and from their genetic parents, we found 43 off-targets, 30 of which were predicted by an adapted version of GUIDE-seq