7 research outputs found
Rapid Discovery of Pyrido[3,4- d ]pyrimidine Inhibitors of Monopolar Spindle Kinase 1 (MPS1) Using a Structure-Based Hybridization Approach
Monopolar spindle 1 (MPS1) plays a central role in the transition of cells from metaphase to anaphase and is one of the main components of the spindle assembly checkpoint. Chromosomally unstable cancer cells rely heavily on MPS1 to cope with the stress arising from abnormal numbers of chromosomes and centrosomes and are thus more sensitive to MPS1 inhibition than normal cells. We report the discovery and optimization of a series of new pyrido[3,4-d]pyrimidine based inhibitors via a structure-based hybridization approach from our previously reported inhibitor CCT251455 and a modestly potent screening hit. Compounds in this novel series display excellent potency and selectivity for MPS1, which translates into biomarker modulation in an in vivo human tumor xenograft mode
7-(Pyrazol-4-yl)-3H-imidazo[4,5-b]pyridine-based derivatives for kinase inhibition: Co-crystallisation studies with Aurora-A reveal distinct differences in the orientation of the pyrazole N1-substituent
Introduction of a 1-benzyl-1H-pyrazol-4-yl moiety at C7 of the imidazo[4,5-b]pyridine scaffold provided 7a which inhibited a range of kinases including Aurora-A. Modification of the benzyl group in 7a, and subsequent co-crystallisation of the resulting analogues with Aurora-A indicated distinct differences in binding mode dependent upon the pyrazole N-substituent. Compounds 7a and 14d interact with the P-loop whereas 14a and 14b engage with Thr217 in the post-hinge region. These crystallographic insights provide options for the design of compounds interacting with the DFG motif or with Thr217
Rapid Discovery of Pyrido[3,4‑<i>d</i>]pyrimidine Inhibitors of Monopolar Spindle Kinase 1 (MPS1) Using a Structure-Based Hybridization Approach
Monopolar
spindle 1 (MPS1) plays a central role in the transition
of cells from metaphase to anaphase and is one of the main components
of the spindle assembly checkpoint. Chromosomally unstable cancer
cells rely heavily on MPS1 to cope with the stress arising from abnormal
numbers of chromosomes and centrosomes and are thus more sensitive
to MPS1 inhibition than normal cells. We report the discovery and
optimization of a series of new pyrido[3,4-<i>d</i>]pyrimidine
based inhibitors via a structure-based hybridization approach from
our previously reported inhibitor CCT251455 and a modestly potent
screening hit. Compounds in this novel series display excellent potency
and selectivity for MPS1, which translates into biomarker modulation
in an in vivo human tumor xenograft model
Discovery of a Chemical Probe Bisamide (CCT251236): An Orally Bioavailable Efficacious Pirin Ligand from a Heat Shock Transcription Factor 1 (HSF1) Phenotypic Screen
Phenotypic screens, which focus on
measuring and quantifying discrete
cellular changes rather than affinity for individual recombinant proteins,
have recently attracted renewed interest as an efficient strategy
for drug discovery. In this article, we describe the discovery of
a new chemical probe, bisamide (CCT251236), identified using an unbiased
phenotypic screen to detect inhibitors of the HSF1 stress pathway.
The chemical probe is orally bioavailable and displays efficacy in
a human ovarian carcinoma xenograft model. By developing cell-based
SAR and using chemical proteomics, we identified pirin as a high affinity
molecular target, which was confirmed by SPR and crystallography