Surveillance of anti-HCV antibody amongst in-school youth in a Nigeria university

Infection with Hepatitis C Virus (HCV) is a public health problem. Worldwide, there are about 170 million people infected with HCV. HCV is transmitted through sex and use of contaminated sharp objects during tattooing or intravenous drug abuse. These routes make youth to be more vulnerable. Transfusion and mother to child transmissions are also documented modes. This study was carried out to determine sero-prevalence of hepatitis C virus infection among in school youth at Achievers University, Owo in southwest Nigeria. Samples of blood were collected from 70 undergraduate students and sera harvested were tested for the presence of antibodies against hepatitis C virus by Enzyme Immunoassay Technique. Most participants fall within age range 21-25 (91.4%). The study showed that none of the subjects was positive for anti- HCV antibodies. Education and awareness level might have impacted positively on this outcome. Thus routine screening for HCV and sustained awareness creation activities to eradicate HCV and its attendant consequences from our society is of paramount importance.Key words: HCV, prevalence, in-school youth, education

EKSPERIMENTALNO ISPITIVANJE SINTETIZIRANE ISPLAKE NA BAZI BIODIZELA

Diesel oil has been the preferred base fluid for the formulation of oil-based drilling mud. Diesel oil has negative effects on the environment and there is a growing need for more environmentally sustainable alternatives that can be technically compared to diesel base oil. In recent times, the use of vegetable oils as drilling fluid base oil has been of interest. In this study, 1378 kg/m3 of palm kernel oil-based mud (PKOBM) and palm kernel oil biodiesel based mud (BDBM) were experimentally formulated. BDBM was synthesized by the transesterification of vegetable (palm kernel) oil. The performance of PKOBM and BDBM was then evaluated against conventional diesel oil-based mud (DOBM). The evaluation performed was based on the rheological, filtration and wall building properties, emulsion and thermal stabilities, and acute toxicity of the formulated drilling fluid systems. The results obtained from the study reveal that the mud systems (PKOBM and BDBM) show a typical Herschel-Bulkley (modified power-law) drilling mud rheological pattern at temperatures of 49°C, 66°C and 80°C. BDBM showed comparable rheological properties with better hole cleaning capacity as indicated by a lower flow index. For the filtrate loss test, BDBM exhibited a slightly lower filtrate loss compared to DOBM, whereas PKOBM had a higher fluid loss of 4.4 ml. For the filter cake and thermal stability test, there were no significant changes between DOBM and BDBM, whereas PKOBM had the least desirable performance. BDBM exhibited the most stable emulsion of 1274 volts breaking voltage and PKOBM, the least with 739 volts compared to 1169 volts breaking voltage of DOBM. This study concludes that BDBM could be used as an environmentally sustainable substitute for diesel oil-based mud (DOBM).Kao bazni fluid za pripremu isplake na bazi ulja najčešće se upotrebljava dizelsko ulje. Dizelsko ulje ima negativan utjecaj na okoliš i sve je veća potreba za ekološki održivijim alternativama koje se s tehničkoga aspekta mogu usporediti s dizelskim baznim uljem. U novije se vrijeme istražuje mogućnost upotrebe biljnih ulja kao baznoga ulja u isplakama. U ovome istraživanju eksperimentalno su pripremljene isplaka (gustoće 1378 kg/m3) na bazi palmina ulja (ulje iz palminih koštica) (PKOBM) i isplaka na bazi biodizela dobivenoga iz palmina ulja (BDBM). BDBM je dobiven transesterifikacijom biljnoga (palmina) ulja. Zatim je napravljena usporedba karakteristika PKOBM-a i BDBM-a i konvencionalne isplake na bazi dizelskoga ulja (DOBM). Isplake su uspoređene na temelju reoloških i filtracijskih svojstava, sposobnosti stvaranja isplačnoga obloga, stabilnosti emulzije, toplinske stabilnosti te akutne toksičnosti. Rezultati dobiveni istraživanjem upućuju na to da isplake PKOBM i BDBM pokazuju tipično Herschel-Bulkley (modificirani eksponencijalni (power-law) model) reološko ponašanje pri temperaturama od 49 °C, 66 °C i 80 °C. BDBM je pokazao usporediva reološka svojstva uz bolji kapacitet iznošenja krhotina nego što bi na to upućivao niži indeks protoka. U testu gubitka filtrata isplake BDBM je pokazao nešto manji gubitak filtrata u odnosu na DOBM, dok je PKOBM imao veći gubitak filtrata, koji je iznosio 4,4 ml. Ispitivanja isplačne obloge (filter cake) i termičke stabilnosti nisu pokazala znatnije razlike između DOBM-a i BDBMa, dok je PKOBM imao najmanje poželjne performanse. U usporedbi stabilnosti emulzija BDBM je pokazao najstabilniju emulziju (destabilizacija emulzije pri naponu od 1274 V), a PKOBM najmanje stabilnu emulziju (destabilizacija pri 739 V), dok u slučaju DOBM-a do destabilizacije emulzije dolazi pri naponu od 1169 V. Temeljem provedenih istraživanja zaključeno je da bi se BDBM mogao koristiti kao ekološki održiva zamjena za isplaku na bazi dizelskoga ulja (DOBM)

The Parity Rate of Indoor-Resting Adult Female Anopheles and Culex Mosquitoes and Their Implication in Disease Transmission in Nnamdi Azikiwe University Female Hostels Awka, South Eastern Nigeria

A study on the parity rate of indoor-resting Anopheles and Culex mosquitoes and their implication in disease transmission was carried out in Nnamdi Azikiwe University female hostel between June and July 2016. The mosquitoes were sampled weekly from 24 randomly selected rooms using pyrethrum knock-down collection (P.K.C). A total of 516 mosquitoes comprising of 4 species: Anopheles Gambiae, Anopheles funestus, Culex quinquefasciatus and Culex annulioris, were collected during the study period. The mosquitoes were examined for their abdominal gradings/gonotrophic stages and dissected for parity determination. Culex quinquefasciatus (61.43%) constituted the most abundant species followed by Anopheles Gambiae (30.04%) and Anopheles funestus (7.56%) and the least being Culex annulioris (0.97%). Results showed that majority of the vector species were fed and parous and variations among the parity rates of the 4 species was significant (

Fibrinogen Concentration and Thrombin Levels in Pregnant Women in Nnewi, Anambra State, South, Eastern Nigeria.

This study was aimed at looking at the influence of pregnancy on fibrinogen concentration and thrombin time (TT) levels. A total of 195 pregnant women between 18 and 35 years were recruited, 59 were in their first trimester (group A), 61 second trimester (group B) and 75 the third trimester (group C). A total of 150 apparently healthy non pregnant women were used as normal control (group D). Blood samples which was collected from each of the participants after obtaining informed consent was tested for the fibrinogen concentration using Clauss method and thrombin time using two stage method. The study was approved by Nnamdi Azikiwe University Teaching Hospital Ethical Committee Nnewi. ANOVA and students t-test were used for statistical analysis. The results showed that fibrinogen concentration (mg/dL) were significantly higher (p<0.05) in group A, B and C as compared with the control group. TT (s) were significantly higher (p<0.05) in group A, B and C compared with the control group. This study therefore suggests that the increase in these coagulation factors observed are due to increased thrombin generation, inflammatory state of pregnancy and fibrinogen being an acute-phase protein. It is important to obtain a baseline of these parameters for all pregnant women during antenatal visits, in order to detect any abnormality early. Key words: Fibrinogen concentration, thrombin time and pregnancy

Hydrochemical Characteristics of Water Quality Around Nkalagu Area, Southern Benue Trough, Nigeria Using Multivariate Statistical Analysis

Hydrochemical characteristics of water quality around Nkalagu area has been studied and characterized using multivariate statistical analysis. Eighty water samples were collected in the area from spatially referenced boreholes, hand dug wells abandoned mines, catch pits and rivers located in and around the Nkalagu area and were analyzed for EC, pH, TDS, TH, Ca2+, Mg2+, Na+, K+, HCO3-, Cl-, NO3-, SO42- and Fe2+ according to EPA and APHA standards. Based on mean values, the order of abundance in ions is Cl- > HCO3- > SO42- > NO3-, for anions and Na+ > Ca2+ > Mg2 - > K+, for cations. TDS and Salinity hazard classifications characterized the water in the study area as soft to very hard with low to very high salinity hazard. Principal component analysis (PCA) reduced the hydrochemical data into two principal components which explain 78.553 %, of the total variance that characterize the water quality in relation to the source of its hydrochemistry. Cluster analysis (CA) grouped eighty water samples in the area into eight clusters of similar water quality characteristics related to water-rock interaction, agriculture and anthropogenic sources. Discriminant analysis (DA) showed that the discriminating parameters of water quality in the area are EC, TDS, TH, SO4, Cl, Mg, Ca, Na, and HCO3 and this revealed that water quality in the area is controlled by both geogenic and anthropogenic processes. Keywords: Water Quality, Multivariate analysis, cluster analysis, principal component analysis DOI: 10.7176/JEES/9-11-07 Publication date: November 30th 201

Amphipathic anionic surfactant modified hydrophilic polyethylene glycol-nanosilica composite as effective viscosifier and filtration control agent for water-based drilling muds

Highly stabilized and dispersible composites of polyethylene glycol and silica nanoparticle in aqueous drilling mud can provide desirable rheological and filtration properties for drilling jobs. Therefore, high-quality hydrophilic polyethylene glycol-nanosilica composite modified by amphipathic anionic sodium dodecyl sulfate (PEG-SiO2 NC-SDS) to improve the rheological and filtration properties of water-based muds (WBMs) was submitted. Test of zeta potential, functional groups, morphology, elemental composition, and temperature stability together with rheology and filtration tests were undertaken to assess the wide-ranging mud properties of the SDS modified PEG-SiO2 NC drilling muds. Zeta potential, FTIR, FESEM, EDX, and TGA results indicate that the SDS modified PEG-SiO2 NC was effectively formed and modified, it embodies exceptional thermal stability and is efficiently dispersed. The SDS modified PEG-SiO2 NC has a narrow size distribution range between 82 nm and 410 nm, and a specific surface area of 41.4 m2/g that is sufficiently high for particle-molecule interactions. Its rheological variables are notably shear-thinning and did not undergo notable fluctuation. The filtrate loss of 1.5 g SDS bearing PEG-SiO2 NC at 78 °F and 250 °F was only 5.4 ml and 9.6 ml, against 10.2 ml and 20.5 ml of the WBMs, respectively. High dispersion stability and high thermal stability aided its excellent viscosity and filtration control performance. Moreover, optimum rheological properties for the SDS modified PEG-SiO2 NC drilling muds with Bingham plastic and Ostwald-de-Waele models occurred with mud composition CD3 (CD3 = 1.5 g SDS modified PEG-SiO2 NC + WBM). Thus, this study can help to understand the applications of this nanocomposite as a potential viscosifier and filtrate loss control material for WBMs

Impact of a quadrivalent meningococcal ACWY glycoconjugate or a serogroup B meningococcal vaccine on meningococcal carriage: an observer-blind, phase 3 randomised clinical trial

Background: Meningococcal conjugate vaccines protect individuals directly, but also confer herd protection by interrupting carriage transmission. This Phase III observer-blind, randomised, controlled study evaluated the effects of meningococcal quadrivalent (ACWY) glycoconjugate (MenACWY-CRM) or serogroup B (4CMenB) vaccination on meningococcal carriage rates in young adults. Methods: University students (aged 18–24 years) from ten sites in England were randomised to receive two vaccinations one month apart: two doses of Japanese Encephalitis vaccine (controls), two doses of 4CMenB (4CMenB), or one dose of MenACWY-CRM then placebo (MenACWY-CRM). Meningococci were isolated from oropharyngeal swabs collected before vaccination and at five scheduled intervals over one year. Primary analysis was cross-sectional carriage one month after the vaccine course; secondary analyses included comparison of carriage at any time point after primary analysis until study termination. Findings: 2954 subjects were randomised (control, n=987; 4CMenB, n=988; MenACWY-CRM, n=979); approximately one-third of each group was positive for meningococcal carriage at study entry. By one month, there was no significant difference in carriage between controls and 4CMenB (Odds Ratios (OR) [95% CI]; 1·2 [0·8−1·7]) or MenACWY-CRM (OR [95% CI], 0·9 [0·6–1·3]) groups. From three months after dose two, 4CMenB vaccination resulted in significantly lower carriage of any meningococcal strain (calculated efficacy 18·2% [95% CI: 3·4–30·8]) and capsular groups BCWY (calculated efficacy 26·6% [95% CI: 10·5–39·9]) compared to control vaccination. Significantly lower carriage rates were also observed in the MenACWY-CRM group compared with controls: calculated efficacies 39·0% [95%CI: 17·3-55·0] and 36.2% [95%CI: 15·6-51·7] for serogroups Y and CWY, respectively. Interpretation: MenACWY-CRM and 4CMenB vaccines reduced meningococcal carriage rates over 12 months post-vaccination and, therefore, may affect transmission where widely implemented

Effect of a quadrivalent meningococcal ACWY glycoconjugate or a serogroup B meningococcal vaccine on meningococcal carriage: an observer-blind, phase 3 randomised clinical trial

Background: Meningococcal conjugate vaccines protect individuals directly, but also confer herd protection by interrupting carriage transmission. This Phase III observer-blind, randomised, controlled study evaluated the effects of meningococcal quadrivalent (ACWY) glycoconjugate (MenACWY-CRM) or serogroup B (4CMenB) vaccination on meningococcal carriage rates in young adults.Methods: University students (aged 18–24 years) from ten sites in England were randomised to receive two vaccinations one month apart: two doses of Japanese Encephalitis vaccine (controls), two doses of 4CMenB (4CMenB), or one dose of MenACWY-CRM then placebo (MenACWY-CRM). Meningococci were isolated from oropharyngeal swabs collected before vaccination and at five scheduled intervals over one year. Primary analysis was cross-sectional carriage one month after the vaccine course; secondary analyses included comparison of carriage at any time point after primary analysis until study termination.Findings: 2954 subjects were randomised (control, n=987; 4CMenB, n=988; MenACWY-CRM, n=979); approximately one-third of each group was positive for meningococcal carriage at study entry. By one month, there was no significant difference in carriage between controls and 4CMenB (Odds Ratios (OR) [95% CI]; 1·2 [0·8−1·7]) or MenACWY-CRM (OR [95% CI], 0·9 [0·6–1·3]) groups. From three months after dose two, 4CMenB vaccination resulted in significantly lower carriage of any meningococcal strain (calculated efficacy 18·2% [95% CI: 3·4–30·8]) and capsular groups BCWY (calculated efficacy 26·6% [95% CI: 10·5–39·9]) compared to control vaccination. Significantly lower carriage rates were also observed in the MenACWY-CRM group compared with controls: calculated efficacies 39·0% [95%CI: 17·3-55·0] and 36.2% [95%CI: 15·6-51·7] for serogroups Y and CWY, respectively.Interpretation: MenACWY-CRM and 4CMenB vaccines reduced meningococcal carriage rates over 12 months post-vaccination and, therefore, may affect transmission where widely implemented

Secondary attack rates in primary and secondary school bubbles following a confirmed case: Active, prospective national surveillance, November to December 2020, England.

BACKGROUND: Following the full re-opening of schools in England and emergence of the SARS-CoV-2 Alpha variant, we investigated the risk of SARS-CoV-2 infection in students and staff who were contacts of a confirmed case in a school bubble (school groupings with limited interactions), along with their household members. METHODS: Primary and secondary school bubbles were recruited into sKIDsBUBBLE after being sent home to self-isolate following a confirmed case of COVID-19 in the bubble. Bubble participants and their household members were sent home-testing kits comprising nasal swabs for RT-PCR testing and whole genome sequencing, and oral fluid swabs for SARS-CoV-2 antibodies. RESULTS: During November-December 2020, 14 bubbles were recruited from 7 schools, including 269 bubble contacts (248 students, 21 staff) and 823 household contacts (524 adults, 299 children). The secondary attack rate was 10.0% (6/60) in primary and 3.9% (4/102) in secondary school students, compared to 6.3% (1/16) and 0% (0/1) among staff, respectively. The incidence rate for household contacts of primary school students was 6.6% (12/183) and 3.7% (1/27) for household contacts of primary school staff. In secondary schools, this was 3.5% (11/317) and 0% (0/1), respectively. Household contacts were more likely to test positive if their bubble contact tested positive although there were new infections among household contacts of uninfected bubble contacts. INTERPRETATION: Compared to other institutional settings, the overall risk of secondary infection in school bubbles and their household contacts was low. Our findings are important for developing evidence-based infection prevention guidelines for educational settings