Analysis of Sector Led Improvement of Children and Young People’s Services

This paper was commissioned by the Association of Directors of Children’s Services (ADCS) National Performance and Information Management Group (NPIMG) to support the work of ADCS to evaluate and assess the impact of Sector Led Improvement of Children and Young People’s Services, and to explore the possibility of designing and developing (with ADCS and other partners) an ‘Early Warning’ model. The objective is to identify when and where the performance of Children and Young People’s Services is moving in the wrong direction. We undertake pre-post testing and difference in difference analysis, the latter allows us to use control data to ensure that external factors affecting all regions are considered

NPI-1, the human homolog of SRP-1, Interacts with influenza virus nucleoprotein

AbstractWe used the yeast interactive trap system to identify a cellular protein which interacts with the nucleoprotein of influenzaA viruses. This protein, nucleoprotein interactor 1 (NPI-1) is the human homolog of the yeast protein SRP1. SRP1 was previously identified as a suppressor of temperature-sensitive RNA polymerase I mutations (R. Yano, M. Oakes, M. Yamaghishi, J. Dodd, and M. Nomura, Mol. Cell. Biol. 12, 5640–5651, 1992). A full-length cDNA clone of NPI-1 was generated from HeLa cell poly A+ RNA. The viral nucleoprotein, which had been partially purified from influenza A/PR/8/34 virus-infected embryonated eggs, could be coprecipitated from solution by glutathione agarose beads complexed with a bacterially expressed glutathione-S-transferase-NPI-1 fusion protein, confirming the' results of the yeast genetic system. Antisera raised against NPI-1 identified a 60-kDa polypeptide from total cellular extracts of both HeLa and MDBK cells. The viral nucleoprotein was coimmunoprecipitated from influenza A/WSN/33 virus-infected MDBK cells by anti-NPI-1 sera, demonstrating an interaction of these two proteins in infected cells. Similarly, NPI-1 was coimmunoprecipitated from MDBK cells by anti-NP sera. These experiments suggest that NPI-1 plays a role during influenza virus replication

Preparing to Face Our Next Enemy

Emeritus Professor Robert O'Neill, a former head of SDSC examines the current and future requirements of the Australian Defence Force. O'Neill argues that the ADF must be able to defend the continent, assist international forces, and conduct counter-insurgency and disaster relief

Venting, joining and educating: motivations for knowledge sharing in the UK police blogosphere.

This article examines motivations for knowledge sharing in blogs written by police officers. It draws on the findings of a research project completed in 2011 based on content analysis of 63 blogs

Sample mounts for microcrystal crystallography

Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity

Sample mounts for microcrystal crystallography

Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity