Fluorescence-based analyser as a rapid tool for determining soluble protein content in dairy ingredients and infant milk formula

peer-reviewedAbstract: Milk protein, in particular native whey protein, is of interest to dairy manufacturers as a measure of functional and nutritional quality. However, quantification of soluble whey protein (SP) is time consuming; giving rise to the need to develop rapid, accurate, and portable at-line process analytical technology. In this study, the performance of a fluorescence-based analyser(F) (Amaltheys II, Spectralys Innovations, France) was evaluated for quantification of SPF and whey protein nitrogen index (WPNI)F in skim milk, whey protein concentrate and infant formula powders. Rehydration of powders prior to analysis was a key factor for ensuring repeatability and reproducibility. A comparison of the analyser with reference methods for SPF and WPNIF resulted in coefficient of determination (R2) > 0.993 for both SPKjeldahl method and WPNIGEA. The results show the fluorescence-based analyser to be rapid, compact, and accurate device, suited for providing reliable support to dairy ingredient and infant formula manufacturers. Industrial relevance: The fluorescence based analysis investigated in this article is suitable for application in the dairy industry where it can be used as a rapid, at-line PAT tool for both liquid and powder samples. The technology has the potential to replace well-established methods for measurement of soluble protein. The main benefit to industry is the ability to respond more rapidly to variations in soluble protein without compromising on the accuracy associated with more time consuming methods

A serological survey of ruminant livestock in Kazakhstan during post-Soviet transitions in farming and disease control

The results of a serological survey of livestock in Kazakhstan, carried out in 1997–1998, are reported. Serum samples from 958 animals (cattle, sheep and goats) were tested for antibodies to foot and mouth disease (FMD), bluetongue (BT), epizootic haemorrhagic disease (EHD), rinderpest (RP) and peste des petits ruminants (PPR) viruses, and to Brucella spp. We also investigated the vaccination status of livestock and related this to changes in veterinary provision since independence in 1991. For the 2 diseases under official surveillance (FMD and brucellosis) our results were similar to official data, although we found significantly higher brucellosis levels in 2 districts and widespread ignorance about FMD vaccination status. The seroprevalence for BT virus was 23%, and seropositive animals were widespread suggesting endemicity, despite the disease not having being previously reported. We found a few seropositives for EHDV and PPRV, which may suggest that these diseases are also present in Kazakhstan. An hierarchical model showed that seroprevalence to FMD and BT viruses were clustered at the farm/village level, rather than at a larger spatial scale. This was unexpected for FMD, which is subject to vaccination policies which vary at the raion (county) level

A study of factors affecting the efficiency of milking operations.

End of Project ReportWith a mid-level milking system the milking time was reduced significantly when the teat end vacuum was increased • Vacuum losses were lower and milking time was shorter with simultaneous pulsation than with alternate pulsation • Milk yield was not affected by the magnitude of teat end vacuum. • Both the mean flowrate and peak flowrate increased when the teat end vacuum was increased. • New milking plants and conversions should have 16 mm bore long milk tubes (LMT) and 16 mm bore entries in the milk pipeline • The omission of udder washing as a pre-milking preparation procedure did not influence milking characteristics. • TBC and E. coli were significantly reduced with full pre-milking preparation compared to no pre-milking preparation when milk was produced from cows on pasture • Counts for individual bacterial species were well below maximum numbers permitted in EU Council Directive (Anon. 1992) when no pre-milking preparation was carried out

Evaluation of Milking Systems in Terms of Mastitis Risk, Teat Tissue Reactions & Milking Performance.

End of Project ReportsMeasurements of milking vacuum recorded on a flow simulator can provide guidelines for optimum design of milking units. • Increasing the bore of the short milk tube above the recommended diameter or claw volume above 150ml does not improve milking efficiency. • Increasing the long milk-tube bore from 13.5mm to 16mm increased the level of milking vacuum. • The milking vacuum was highest with wide-bore tapered liners and simultaneous pulsation. • The minimum vacuum was increased with narrow-bore liners and alternate pulsation. • The milk yield with wide-bore tapered liners in heavy 3-kg clusters and using simultaneous pulsation was 5% higher than with light clusters (1.65 kg) with alternate pulsation. • The milk yield depressions obtained with light clusters were similar in short and long term experiments and increased with the magnitude of the milk yield per milking. • The teat condition scores were not affected by the magnitude of vacuum fluctuations

Relationships of Maternal and Paternal Anthropometry With Neonatal Body Size, Proportions and Adiposity in an Australian Cohort

The patterns of association between maternal or paternal and neonatal phenotype may offer insight into how neonatal characteristics are shaped by evolutionary processes, such as conflicting parental interests in fetal investment and obstetric constraints. Paternal interests are theoretically served by maximizing fetal growth, and maternal interests by managing investment in current and future offspring, but whether paternal and maternal influences act on different components of overall size is unknown. We tested whether parents' prepregnancy height and body mass index (BMI) were related to neonatal anthropometry (birthweight, head circumference, absolute and proportional limb segment and trunk lengths, subcutaneous fat) among 1,041 Australian neonates using stepwise linear regression. Maternal and paternal height and maternal BMI were associated with birthweight. Paternal height related to offspring forearm and lower leg lengths, maternal height and BMI to neonatal head circumference, and maternal BMI to offspring adiposity. Principal components analysis identified three components of variability reflecting neonatal “head and trunk skeletal size,” “adiposity,” and “limb lengths.” Regression analyses of the component scores supported the associations of head and trunk size or adiposity with maternal anthropometry, and limb lengths with paternal anthropometry. Our results suggest that while neonatal fatness reflects environmental conditions (maternal physiology), head circumference and limb and trunk lengths show differing associations with parental anthropometry. These patterns may reflect genetics, parental imprinting and environmental influences in a manner consistent with parental conflicts of interest. Paternal height may relate to neonatal limb length as a means of increasing fetal growth without exacerbating the risk of obstetric complications. Am J Phys Anthropol 156:625–636, 2015. © 2014 The Authors American Journal of Physical Anthropology Published by Wiley Periodicals, Inc

High speed video capture for mobile phone cameras

We consider an electromechanical model for the operation of a voice coil motor in a mobile phone camera, with the aim of optimizing how a lens can be moved to a desired focusing motion. Although a methodology is developed for optimizing lens shift, there is some concern about the experimentally-determined model parameters that are at our disposal. Central to the model is the value of the estimated magnetic force constant, Kf: its value determines how far it is actually possible to move lens, but it appears that, from the value given, it would not be possible to shift the lens through the displacements desired. Furthermore, earlier experiments have also estimated the value of the back EMF constant, Kg , to be roughly five times greater than Kf, even though we present two theoretical arguments that show that Kf = Kg: a conclusion supported by readily-available manufacturers’ data

Advantages of the net benefit regression framework for trial-based economic evaluations of cancer treatments: an example from the Canadian Cancer Trials Group CO.17 trial.

BackgroundEconomic evaluations commonly accompany trials of new treatments or interventions; however, regression methods and their corresponding advantages for the analysis of cost-effectiveness data are not widely appreciated.MethodsTo illustrate regression-based economic evaluation, we review a cost-effectiveness analysis conducted by the Canadian Cancer Trials Group's Committee on Economic Analysis and implement net benefit regression.ResultsNet benefit regression offers a simple option for cost-effectiveness analyses of person-level data. By placing economic evaluation in a regression framework, regression-based techniques can facilitate the analysis and provide simple solutions to commonly encountered challenges (e.g., the need to adjust for potential confounders, identify key patient subgroups, and/or summarize "challenging" findings, like when a more effective regimen has the potential to be cost-saving).ConclusionsEconomic evaluations of patient-level data (e.g., from a clinical trial) can use net benefit regression to facilitate analysis and enhance results

The Application of On-line Sensors and Novel Control Technologies for Food Processing

End of Project ReportThe objective of this research was to apply on-line continuous sensors in food processing, in particular in cheese and milk powder manufacture, in order to improve process control, for example, by achieving higher quality, increased yields, reduced losses and less downgrading of product. This project focused on technologies for monitoring rheologyrelated parameters. The main conclusions were as follows: * Seven systems for monitoring curd formation in cheesemaking were evaluated in the laboratory. * Two on-line systems for monitoring curd firmness (hot-wire and NIR reflectance) have been deployed in a commercial cheese plant with promising results. * Experimental results demonstrated that NIR reflectance / transmission probes have a potential for on-line application in cheesemaking. Despite the difference in scale, the commercial sensors compared well with the cheesemaker s observation of curd firming and look promising as an objective means of predicting curd cut time in an industrial cheese plan. * A detailed knowledge of the rheological variation in cheese curd has been developed and a means of investigating factors which influence the rheology of cheese curd (e.g. effect of heat treatment or fortification of cheesemilk) has been determined. * Technologies available for monitoring concentrate viscosity changes in the production of milk powder have been assembled at pilot scale, and initial trials have been encouraging. Further evaluation of the MTL plant to assess on-line performance, ruggedness and cleanability are planned.Department of Agriculture, Food and the Marin