13 research outputs found
Bioassay of Geniculosporium species for Phytophthora megakarya biological control on cacao pod husk pieces
Fungal endophytes (Geniculosporium sp.) isolated from cacao leaves were screened for biological control of Phytophthora megakarya the cacao black pod disease pathogen, using Cacao Pod Husk Pieces (CPHP). CPHP were pre-treated with spore suspensions of Geniculosporium sp. [BC13 (GJS 01- 196), BC108 (GJS 01-192), BC118 (GJS 01-197), BC177 (GJS 01-198)], and were infected with P. megakarya zoospore suspensions (105 zoospores/ml). Effects on P. megakarya were noticed at pod infection, mycelia growth inside infected tissues, and fungal sporulation, that are major stages of the black pod disease cycle on cacao pods. CPHP pre-treated with BC108 expressed the lowest early Infection Index (EII), but could not control disease progress into infected cacao pod husk tissues. On CPHP pre-treated with BC13, average growth rate of the necrosis was significantly reduced, but no control on P. megakarya sporulation was observed, while CPHP pre-treated with BC177 significantly reduced P. megakarya sporulation.Key words: Cacao, biological control, Geniculosporium sp, endophytes, Phytophthora megakarya, pod husks pieces
Full Length Research Paper - Bioassay of Geniculosporium species for Phytophthora megakarya biological control on cacao pod husk pieces
Fungal endophytes (Geniculosporium sp.) isolated from cacao
leaves were screened for biological control of Phytophthora megakarya
the cacao black pod disease pathogen, using Cacao Pod Husk
Pieces (CPHP). CPHP were pre-treated with spore suspensions of
Geniculosporium sp. [BC13 (GJS 01196), BC108 (GJS 01-192), BC118 (GJS
01-197), BC177 (GJS 01-198)], and were infected with P. megakarya
zoospore suspensions (105 zoospores/ml). Effects on P. megakarya were
noticed at pod infection, mycelia growth inside infected tissues, and
fungal sporulation, that are major stages of the black pod disease
cycle on cacao pods. CPHP pre-treated with BC108 expressed the lowest
early Infection Index (EII), but could not control disease progress
into infected cacao pod husk tissues. On CPHP pre-treated with BC13,
average growth rate of the necrosis was significantly reduced, but no
control on P. megakarya sporulation was observed, while CPHP
pre-treated with BC177 significantly reduced P. megakarya sporulation