Densidade de energia e aminoácidos em dietas de leitões na fase pré-inicial de creche: desempenho e composição química corporal

It was evaluated the effects of metabolizable energy (ME) and digestible lysine (dLYS) densities on performance and body composition of weaned piglets. The study used 114 piglets weaned at 7.4 ± 0.80 kg, out of which 108 were allotted in the nursery and 6 were slaughtered on the weaning day to determine comparative data of body chemical composition. Six nutrients densities were stipulated from a previous study based on the highest nitrogen retention, maintaining the following ME:LYS relationship in the experimental diets: 3,390:1.291; 3,450:1.409; 3,650:1.411; 3,780:1.461; 3,940:1.507; and 4,109 kcal/kg ME:1.564% dLYS. The experimental diets were offered for 13 days when the piglets reached 12.986 ± 1.449 kg of body weight. The probable residual effects of nutritional density on the subsequent performance of the piglets were evaluated. At the end of initial phase 1, six piglets from each density were slaughtered to determine their chemical composition in body fractions and empty body. There was no significant influence of nutritional levels on the performance of the piglets at the end of the evaluation. The results of food conversion and body composition confirm the level indicated in the previous study, 4 g dLYS/Mcal of ME. The increase of energy and lysine densities confirms the need for a correct relationship among both of them to assure better performance of the piglets at the beginning of the growing phase.Avaliaram-se os efeitos da densidade de energia metabolizável (EM) e lisina digestível (LIS) sobre o desempenho e a composição corporal de leitões após o desmame. Utilizaram-se 114 animais desmamados aos 7,4 ± 0,80 kg; desses animais, 108 foram alojados na unidade de creche e 6 foram abatidos no dia do desmame para determinação dos dados comparativos da composição química corporal. Seis densidades de nutrientes foram estipuladas a partir de estudo anterior, com base na maior retenção de nitrogênio, mantendo-se as seguintes relações EM:LIS nas dietas experimentais: 3.390:1,291; 3.450:1,409; 3.650:1,411; 3.780:1,461; 3.940:1,507; e 4.109 kcal/kg EM:1,564% LIS. As dietas experimentais foram oferecidas durante 13 dias, quando os leitões atingiram o peso de 12,986 ± 1,449 kg. Avaliaram-se os prováveis efeitos residuais da densidade nutricional no desempenho subseqüente dos leitões. Ao término da fase inicial-1, seis leitões de cada densidade foram abatidos para determinação da composição química nas frações corporais e no corpo vazio. Não houve influência significativa dos níveis nutricionais no desempenho dos leitões ao término da avaliação. Os resultados de conversão alimentar e composição corporal ratificam o nível indicado em estudo anterior, de 4 g LIS/Mcal. O aumento da densidade de energia e lisina confirma a necessidade da correta relação entre ambos para assegurar o melhor desempenho dos leitões na fase inicial de crescimento.Brasil - Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP

Therapeutic Effects of Glutamic Acid in Piglets Challenged with Deoxynivalenol

The mycotoxin deoxynivalenol (DON), one of the most common food contaminants, primarily targets the gastrointestinal tract to affect animal and human health. This study was conducted to examine the protective function of glutamic acid on intestinal injury and oxidative stress caused by DON in piglets. Twenty-eight piglets were assigned randomly into 4 dietary treatments (7 pigs/treatment): 1) uncontaminated control diet (NC), 2) NC+DON at 4 mg/kg (DON), 3) NC+2% glutamic acid (GLU), and 4) NC+2% glutamic acid + DON at 4 mg/kg (DG). At day 15, 30 and 37, blood samples were collected to determine serum concentrations of CAT (catalase), T-AOC (total antioxidant capacity), H2O2 (hydrogen peroxide), NO (nitric oxide), MDA (maleic dialdehyde), DAO (diamine oxidase) and D-lactate. Intestinal morphology, and the activation of Akt/mTOR/4EBP1 signal pathway, as well as the concentrations of H2O2, MDA, and DAO in kidney, liver and small intestine, were analyzed at day 37. Results showed that DON significantly (P<0.05) induced oxidative stress in piglets, while this stress was remarkably reduced with glutamic acid supplementation according to the change of oxidative parameters in blood and tissues. Meanwhile, DON caused obvious intestinal injury from microscopic observations and permeability indicators, which was alleviated by glutamic acid supplementation. Moreover, the inhibition of DON on Akt/mTOR/4EBP1 signal pathway was reduced by glutamic acid supplementation. Collectively, these data suggest that glutamic acid may be a useful nutritional regulator for DON-induced damage manifested as oxidative stress, intestinal injury and signaling inhibition

Effects of dietary threonine:lysine ratioes and sanitary conditions on performance, plasma urea nitrogen, plasma-free threonine and lysine of weaned pigs

Two 21 d-experiments were conducted to determine the optimum standardized ileal digestible (SID) threonine:lysine ratio (Thr:Lys) for weaned piglets reared under clean (Exp. 1) or unclean (Exp. 2) sanitary conditions and fed antibiotic-free diets. In each experiment, 90 mixed-sex pigs (Duroc × [Yorkshire × Landrace]; initial BW 7.2 ± 0.3 kg) were randomly assigned to 5 dietary treatments each with 6 replicates (3 pigs per pen). The dietary treatments were 5 graded levels of SID Thr:Lys (55, 59, 63, 67 and 71%). Diets were corn-wheat-soybean meal-based with a constant SID Lys of 1.18% that was set to be second limiting amino acid. In Exp. 1 and Exp. 2, plasma-free Thr increased (P = 0.05) with increasing dietary SID Thr:Lys. In Exp. 1, the SID Thr:Lys for gain-to-feed ratio (G:F) was optimized at 65%. In Exp. 2, the estimated optimal SID Thr:Lys for overall G:F was 66.5%. In conclusion, an average optimal SID Thr:Lys of 65 and 66.5% could be used to optimize feed efficiency for weaned pigs under clean and unclean sanitary conditions, respectively

Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice

Foot-and-mouth disease virus (FMDV) is a highly contagious agent that impacts livestock industries worldwide, leading to significant financial loss. Its impact can be avoided or minimized if the virus is detected early. FMDV detection relies on vesicular fluid, epithelial tags, swabs, serum, and other sample types from live animals. These samples might not always be available, necessitating the use of alternative sample types. Meat juice (MJ), collected after freeze-thaw cycles of skeletal muscle, is a potential sample type for FMDV detection, especially when meat is illegally imported. We have performed experiments to evaluate the suitability of MJ for FMDV detection. MJ was collected from pigs that were experimentally infected with FMDV. Ribonucleic acid (RNA) was extracted from MJ, sera, oral swabs, and lymph nodes from the same animals and tested for FMDV by real-time reverse transcription polymerase chain reaction (rRT-PCR). MJ was also tested for FMDV antigen by Lateral Flow Immunoassay (LFI). FMDV RNA was detected in MJ by rRT-PCR starting at one day post infection (DPI) and as late as 21 DPI. In contrast, FMDV RNA was detected in sera at 1&ndash;7 DPI. Antigen was also detected in MJ at 1&ndash;9 DPI by LFI. Live virus was not isolated directly from MJ, but was recovered from the viral genome by transfection into susceptible cells. The data show that MJ is a good sample type for FMDV detection

Deoxynivalenol content in different dietary groups.

<p>Pigs (7 pigs/group) were fed with uncontaminated basal diet (NC), or basal diet contaminated with deoxynivalenol at dose of 4 mg/kg (DON), or uncontaminated basal diet supplemented with 2% glutamic acid (GLU), or DON diet supplemented with 2% glutamic acid (DG). Deoxynivalenol content was determined using an assay kit in accordance with the manufacturer’s instructions (Cusabio, China).</p

Liver and kidney anti-oxidative capacity in each group.

<p>A: MDA concentrations of liver and kidney in each group. B: H₂O₂ concentrations of liver and kidney in each group. C: T-AOC of liver and kidney in each group. Dietary treatments were NC, an uncontaminated basal diet, DON, the basal contaminated with 4 mg/kg deoxynivalenol, GLU, uncontaminated basal diet with 2% (g/g) glutamic acid supplementation, and DG, deoxynivalenol-contaminated (4 mg/kg) basal diet with 2% (g/g) glutamic acid supplementation. Data are presented as means ± SEM, n = 7, with a–b used to indicate a statistically significant difference (P<0.05, one way ANOVA method). MDA: methane dicarboxylic aldehyde (nmol/ml); H₂O₂: hydrogen peroxide (mmol/L); T-AOC: total antioxidant capacity (U/mg).</p

Effect of dietary supplementation with glutmic acid on serum amino acids levels (µg/ml) in piglets fed a deoxynivalenol-contaminated diet on day 15.

<p>NC = uncontaminated basal diet, DON = basal diet contaminated with deoxynivalenol (4 mg/kg), GLU = uncontaminated basal diet supplemented with 2% glutamic acid.; DG = DON diet supplemented with 2% glutamic acid. Data are presented as means ± SEM, n = 7, with a–b used to indicate a statistically significant difference (P<0.05, one way ANOVA method).</p

Composition and nutrient levels of basal diet (as-fed basis)¹.

1<p>NC = uncontaminated basal diet, DON = basal diet contaminated with deoxynivalenol (4 mg/kg), GLU = uncontaminated basal diet supplemented with 2% glutamic acid; DG = DON diet supplemented with 2% glutamic acid.</p>2<p>Providing the following amount of vitamins and minerals per kilogram on an as-fed basis: Zn (ZnO), 50 mg; Cu (CuSO₄), 20 mg; Mn (MnO), 55 mg; Fe (FeSO₄), 100 mg; I (KI), 1 mg; Co (CoSO₄), 2 mg; Se (Na₂SeO₃), 0.3 mg; vitamin A, 8,255 IU; vitamin D₃, 2,000 IU; vitamin E, 40 IU; vitamin B₁, 2 mg; vitamin B₂, 4 mg; pantothenic acid, 15 mg; vitamin B₆, 10 mg; vitamin B₁₂, 0.05 mg; vitamin PP, 30 mg; folic acid, 2 mg; vitamin K₃, 1.5 mg; biotin, 0.2 mg; choline chloride, 800 mg; and vitamin C, 100 mg. The premix did not contain additional copper, zinc, antibiotics, or probiotics.</p><p>CP: Crude Protein; DE: Digestible Energy; TP: Total Phosphorus; AP: Available Phosphorus.</p