56 research outputs found
L'istofisiologia della corteccia surrenale
A considerable number of problems related to the histophysiology of the adrenal cortex is updated and discussed in full detail
Ovariectomy-induced changes in the adrenal cortex of spontaneously hypertensive rats
Many lines of evidence indicate that
adrenocortical steroid hormones are involved in the
development and maintenance of hypertension in
spontaneously hypertensive rats (SHR). Twenty-eight
days after ovariectomy a notable decrease in the sistolic
blood pressure (BP) was found in SHR, along with a
marked atrophy of their adrenal cortices. The hypothesis
is advanced that the ovariectomy-induced lowering in
BP in SHR may be, at least partly, mediated by the
suppression of the adrenal secretory activity, due to the
lack of circulating estrogens, which are well known to
stimulate hypophyseal ACTH release
Different effects of neurotensin and neuromedin-N on the proliferative activity of rat adrenal cortex
Evidence indicates that neurotensin (NT) and
neuromedin-N (NMN) exerts an adrenocorticotropic
effect in the rat. The present study aimed to investigate
whether these neuropeptides are able to stimulate the
proliferation of rat adrenocortical cells in vivo and
to compare their mode of action. Adrenocortical
proliferative activity was assessed by the metaphasearrest
technique and metaphases were counted
per medulla-containing adrenal section. A bolus
administration of NT (3 pglrat) resulted in a significant
increase in the number of metaphases in both zona
fasciculata and the entire cortex, an effect observed 48 h
after the in.jection. The administration of NMN (3 yglrat)
induced a notable rise in the number of metaphases in
the zona fasciculata and the entire cortex within 12 h,
followed by a subsequent drop after 24 h and a return to
normal values at 48 h. These findings indicate that NT
and NMN enhance rat adrenal growth in vivo acting via
different mediators
Proliferation and distribution of adrenocortical cells in the gland of ACTH- or dexamethasone-treated rats
The effects of prolonged (7-day) ACTH and
dexamethasone administrations on rat adrenocorticalcell
turnover have been investigated by combined
stereological and metaphase-arrest techniques. ACTH
was found to increase the number of parenchymal cells in
each adrenal zone; however, ACTH altered the cell
distribution in the cortex, lowering their percentage in
the zona glomerulosa (ZG) and zona fasciculata (ZF)
and enhancing it in the zona reticularis (ZR). The cell
birth-rate was markedly raised by ACTH exclusively in
ZG and ZF. Dexamethasone notably decreased the
number of ZF and ZR cells, without altering that of ZG
cells. Moreover. dexamethasone increased the percentage
of parenchymal cells in ZG and ZF, and lowered
it in ZR. In the adrenal cortices of dexamethasoneadministered
animals, metaphases were virtually absent.
These data indicate that ACTH increases the cell birthrate
in ZG and possibly ZF, and enhances the centripetal
migration of newly-formed cells and their accumulation
in ZR. Dexamethasone inhibits both proliferation of
adrenocortical cells in the outer cortical layers and their
centripetal migration into ZR. Moreover, it appears to
cause parenchymal-cell loss in the inner adrenocortical
layers
Nonclassic endogenous regulators of angiogenesis
Angiogenesis, the process through which new blood vessels arise from preexisting ones, is regulated by several "classic" factors, among which the most studied are vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). In recent years, investigations showed that, in addition to the classic factors, numerous endogenous peptides play a relevant regulatory role in angiogenesis. Such regulatory peptides, each of which exerts well-known specific biological activities, are present, along with their receptors, in the blood vessels and may take part in the control of the "angiogenic switch." An in vivo and in vitro proangiogenic effect has been demonstrated for erythropoietin, angiotensin II (ANG-II), endothelins (ETs), adrenomedullin (AM), proadrenomedullin N-terminal 20 peptide (PAMP), urotensin-II, leptin, adiponectin, resistin, neuropeptide-Y, vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating polypeptide (PACAP), and substance P. There is evidence that the angiogenic action of some of these peptides is at least partly mediated by their stimulating effect on VEGF (ANG-II, ETs, PAMP, resistin, VIP and PACAP) and/or FGF-2 systems (PAMP and leptin). AM raises the expression of VEGF in endothelial cells, but VEGF blockade does not affect the proangiogenic action of AM. Other endogenous peptides have been reported to exert an in vivo and in vitro antiangiogenic action. These include somatostatin and natriuretic peptides, which suppress the VEGF system, and ghrelin, that antagonizes FGF-2 effects. Investigations on "nonclassic" regulators of angiogenesis could open new perspectives in the therapy of diseases coupled to dysregulation of angiogenesis
Effects of bombesin and neuromedin-B on the proliferative activity of the rat adrenal cortex
Bombesin (BM) and neuromedin-B (NMB)
exert similar biological effects, acting via two
functionally distinct BM-receptor subtypes. The present
study aimed to investigate whether BM and NMB
stimulate the proliferation of rat adrenocortical cells and
to compare their mode of action. Adult female rats were
treated with a single subcutaneous dose of 3 pg BM or
NMB. Adrenocortical proliferative activity was assessed
by the metaphase-arrest technique. BM administration
resulted in a marked increase in the number of
metaphases in zona glomerulosa (ZG) and zona
fasciculata (ZF), and in the entire cortex. This increase
appeared 24 h after injection in the ZG, and after 48 h in
the ZF. NMB adrninistration, on the other hand, caused a
prompt increase in the number of metaphases in the ZG
and entire cortex at 12 h, followed by a subsequent drop
below the control leve1 at 24 and 48 h of experiment.
These findings indicate that BM and NMB enhance the
proliferative activity of rat adrenocortical cells acting via
different receptors or different mediators
Prolonged kallikrein inhibition does not affect the basal growth and secretory capacity of rat adrenal cortex, but enhances mineralo- and glucocorticoid response to ACTH and handling stress
The effects on the pituitary-adrenocortical functions of the prolonged (7-day) blockade of endogenous bradykinin (BK) synthesis, obtained by the administration of the kallikrein inhibitor (K-I) cyclohexylacetyl-Phe-Arg-Ser-Val-Gln amide, were investigated in the rat. K-I treatment did not cause significant changes in the (i) body and adrenal weights; (ii) basal plasma levels of ACTH, aldosterone and corticosterone; and (iii) average volume of adrenocortical cells and their basal secretory capacity. Conversely, K-I administration induced a significant magnification of the in vivo mineralo- and glucocorticoid responses to the intraperitoneal (i.p.) bolus injection of ACTH. Moreover, K-I-treated rats, but not control ones, displayed a moderate and short-term adrenal secretory response to the mild stress evoked by the placebo i.p. injection. Collectively, these findings rule out the possibility that endogenous BK plays a relevant role in the control of adrenocortical function under basal conditions. However, they suggest that endogenous BK may be involved in quenching exceedingly high adrenocortical responses to ACTH and stresses
Effects of pneumadin (PNM) on the adrenal glands. 5.Potent stimulating action of PNM on adrenocortical growth of dexamethasone-administered rats
Pneumadin (PNM) is a biologically active
decapeptide, originally isolated from mammalian lungs,
that has been previously found to acutely stimulate
pituitary-adrenocortical axis in rats. The effects of 2-day
PNM administration on the atrophic adrenal cortices of
rats treated for 8 days with dexamethasone (DX) were
investigated. PNM significantly raised adrenal weight
and the average volume of adrenocortical cells. The
decapeptide strikingly increased ACTH plasma
concentration; however, the blood levels of aldosterone
and corticosterone, as well as steroid output by adrenal
quarters were not apparently affected. In light of these
findings the following conclusions can be drawn: (i)
PNM enhances the growth of adrenal cortex in DXadministered
rats by a mechanism involving the
stimulation of ACTH release; and (ii) PNM treatment is
probably too short to allow DX-atrophied adrenocortical
cells to re-acquire al1 their differentiated secretory
capacities
Endothelin-1 [1-311 acts as a selective ETA-receptor agonist in the rat adrenal cortex
Endothelin-1 (ET-1) is a 21-amino acid
residue (ET-1[1-211) hypertensive peptide, which
together with its receptor subtypes A and B (ETA and
ETB) is expressed in the rat adrenal cortex, where it
stimulates steroid-hormone (aldosterone and
corticosterone) secretion through the ETB receptor and
the growth (proliferative activity) of the zona
glomerulosa (ZG) through the ETA receptor. ET-1[1-211
is generated from bigET-1 by the endothelin-converting
enzyme (ECE-1). However, recent evidence indicates
the existence of an alternative chymase-mediated
biosynthetic pathway leading to the production of an ET-
1[1-311 peptide, which was found to reproduce the ETA
receptor-mediated vascular effects of ET-l[l-211. We
found that ET-1[1-211, but not ET-1[1-311,
concentration-dependently raised steroid secretion from
dispersed rat adrenocortical cells, its effect being
blocked by the ETB-receptor selective antagonist BQ-
788. Both ET-1s concentration-dependently increased
the number of "S-phase" cells (as detected by the 5-
bromo-2'-deoxyuridine immunocytochemical method)
in capsule-ZG strips within a 240 min incubation. The
ZG proliferogenic action of both ET-1s was blocked by
the ETA-receptor antagonist BQ-123, and ET-l[l-311
was found to be significantly more potent than ET-1[1-
211. Autoradiography showed that in the rat adrenal ET-
1[1-211 displaced the binding of selective ligands to both ETA ( [ 1 2 5 ~ ] ~ ~1-21425) and ETB receptors ( [ 1 2 5 ~ ] ~ ~ -
3020), while ET-l[l-311 eliminates only the binding to
ETA receptors. Collectively, our findings provide strong
evidence that ET-1[1-311 acts in the rat adrenal glands as
a selective ETA-receptor agonist, mainly involved in the
stimulation of ZG proliferative activity
Effects of the prolonged administration of bradykinin on the rat pituitary-adrenocortical axis
The effects of a prolonged administration of
bradykinin (BK) andlor D-Arg, [Hyp3, D - P ~ ~ ~ I - aB K ,
specific antagonist of BK receptors (BK-A) (daily
subcutaneous injections of 4 nmol/rat for 6 days) on the
function of the pituitary-adrenocortical axis were
investigated. BK did not change plasma aldosterone
concentration (PAC), but markedly lowered that of
corticosterone (PBC) and consequently induced a
compensatory hypersecretion of ACTH by the pituitary
gland. BK-A did not apparently affect the function and
growth of the adrenal gland, but, when administered
together with BK, markedly raised both PAC and PBC,
and provoked a significant atrophy of the adrenal gland,
probably due to loss of parenchymal cells. Taken
together, these rather puzzling findings do not appear to
provide clear evidence for the involvement of BK in the
physiological regulation of adrenocortical growth and
steroidogenic capacity in rats
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