Single-row versus double-row arthroscopic rotator cuff repair in small- to medium-sized tears

Hypothesis: Double-row rotator cuff repair leads to superior cuff integrity and clinical results compared with single-row repair

A novel bioreactor design for enhanced aerobic granular sludge formation: effects of cationic polymer and boric acid addition

ozdemir, guven/0000-0002-7577-4233WOS: 000567107300001Compared to the conventional activated sludge, granular sludge has many advantages like good settling ability, high biomass retention and ability to withstand a high organic loading rate. in this study, the effects of boric acid and chitosan addition on granule formation was investigated in a novel Custom-made upflow aerobic granular sludge reactor for 22 days. According to the microscopic results, granulation in all reactors started at 5-7 days. the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) results indicate the evolution of the bacterial community during the granulation process. in addition, high pressure liquid chromatography (HPLC) analysis showed that 100 mg/L of terephthalic acid (TA) was totally degraded in all reactors during 24 hours period. To date, chitosan was generally used in anaerobic granulation studies. Thus, there is little known about the role of chitosan in aerobic granulation. the findings in this study constitute important information about the process of granule formation and the factors that are essential to aerobic granulation

1,3-Propanediol production potential of Clostridium saccharobutylicum NRRL B-643

WOS: 000285896200010PubMed ID: 20647065Owing to the significant interest in biofuel production in the form of biodiesel, vast amount of glycerol as a waste product is produced all over the world Among the economically viable and ecologically acceptable solutions for the safe disposal of this waste, biotechnological conversion of glycerol into a valuable bioplastic raw material, namely 1,3-propanediol (1,3-PDO) seems to be very promising In this study, 1,3-PDO production potential of Clostridium saccharobutylicum NRRL B-643 was studied and the results were compared with other types of anaerobic microorganisms (Clostridium spp, Pantoea agglomerans, Ochrobactrum anthropi, Chyreseomonas luteola, and Klebsiella pneumoniae) and aerobic microorganisms (Lactobacillus spp) The results were important for understanding the significance of C saccharobutylicum NRRL B-643 among other well-known 1,3-PDO producer species According to the screening results only C saccharobutylicum (B-643) was able to consume feed glycerol almost entirely However, 1,3-PDO production yield was found to be 0 36 mol/mol which is lower than that of Clostiridium beijerinckii (B-593) B-593 showed the highest value of production yields with 0 54 mol/mol This microorganism is seen as a promising type for further 1,3-PDO studies, because it has the highest substrate utilization percentage among others In this regard, this microorganism may have an important role in tolerating and converting glycerol during fermentation into 1,3-PD

Purified Terephthalic Acid Wastewater Treatment Using Modified Two-Stage UASB Bioreactor Systems

Ozdemir, Guven/0000-0002-7577-4233WOS: 000520082100001PubMed: 32040764Microbial community dynamics and PTA wastewater degradation performance of sequentially connected two-stage upflow anaerobic sludge blanket (UASB) bioreactors have been studied for 225 days. the working volume of acidogenic (R1) and methanogenic reactors (R2) have sixfold differences. Thus, the reactors operated under different hydraulic retention time (HRT) conditions, which are preferential for PTA wastewater content. Archeal and bacterial profiles of granules were analyzed with denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (Q-PCR) techniques. According to high-pressure liquid chromatography (HPLC) results, 4-Carboxybenzaldehyde (4-CBA) and acetic acid (AA) completely degraded in the first stage, whereas terephthalate (TA) and p-toluic acid (p-TA) degradation ratios were 90% and 47% in the second stage, respectively. the methane content of the UASB reactor was determined as 76% by gas chromatography (GC) analysis. Microbial community analysis indicated that the members of hydrogenotrophic methanogen groups Methanobacteriales and Methanomicrobiales were dominantly responsible for methane production throughout the process.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Y002]; Ege University Scientific Research FundEge University [2013FEN08]; Ege University Science and Technology CenterEge University [2014-BIL-007]The authors wish to thank the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant No. 113Y002 for the financial support of this study. the data presented in this article was produced within the project above; however, it is only the authors of this article who are responsible for the results and discussions made herein. the authors also wish to thank Ege University Scientific Research Fund under the Grant No. 2013FEN08 and Ege University Science and Technology Center (Grant No: 2014-BIL-007) for the financial support of this study

Assessing Organizational Learning in IT Organizations: An experience report from industry

With the increase in demand for higher-quality and more capable IT services, IT organizations in order to obtain competitive advantage require extensive knowledge that needs to be shared and reused among different entities within the organization. The existing IT Service Management (ITSM) mechanisms mention the importance of organizational learning (OL) and knowledge management (KM) for IT organizations. However, they do not explicitly address how OL capabilities of an IT organization can be assessed. This paper, by using an OL assessment model developed for software organizations, namely AiOLoS, shows that with the proper adjustment, the application of the model to IT organizations is feasible. We report the results of applying the model in four functional teams in an IT organization from private sector

Bacterial and archeal dynamics of a labscale HYBRID gas fermentation bioreactor fed with CO2 and H-2

###EgeUn###16s rDNA-based methods were used in order to identify the dynamics of microbial profiles in a HYBRID gas fermentation bio-methanization reactor. The effects of various H-2 and CO2 ratios on microbial community were investigated. The HYBRID gas fermentation reactor was composed of granular anaerobic seed and the system fed with only H-2 and CO2 gases. No additional organic material and trace element was fed during the throughout the experiments; thus, the microbial diversity was directly related to production of methane. The dynamics of the microbial communities were investigated with DGGE and real-time PCR analysis. The results showed that Methanobacteriales members were more dominated than Methanosarcinales and Methanomicrobiales members in the system. DGGE results indicated that Methanosaeta concilii, Methanoculleus sp., Methanosphaerula palustris, Methanofollis formosanus, Methanolinea sp., and Methanobacterium palustre were the most prominent methanogens depending on different H-2/CO2 ratios. DGGE profiles suggested that hydrogenotrophic and acetoclastic species were responsible for the production of methane. The survival of syntrophic bacteria and acetoclastic methanogens was attributed to their utilization of organic materials provided by lysis. To the best of our knowledge, this is the first microbial profile detection study in a hybrid bioreactor system operated with only pure hydrogen and carbon dioxide.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115Y455]; Ege University Scientific Research FundEge University [16/EBILTEM/003]The authors thank Scientific and Technological Research Council of Turkey (TUBITAK) under the grant no. 115Y455 for the financial support of this study. The authors also thank Ege University Scientific Research Fund under the grant no. 16/EBILTEM/003 for the financial support of this study

Comparative analysis of the effect of cell immobilization on the hydrogenothrophic biomethanation of CO2

WOS:000633496000001Carbon capture and utilization (CCU) has been offered as a potential technological solution for mitigating the greenhouse gas emissions and climate change concern worldwide. Anaerobically carbon utilization has started to be in the agenda of researchers in recent years since this approach offers significant advantages such as use of catalysis reactions through environmentally friendly microorganisms under low temperature and pressure operational conditions. Moreover, a cleaner and more effective bioenergy production is realized in the form of biomethane. This study aimed to exploit the merits of cell immobilization in order to provide a stable hydrogenotrophic biomethanation process. For this purpose, two different immobilized bioreactors packed with plastic moving bed biofilm reactor (MBBR) and glass beads packing materials were comparatively investigated. To the best of our knowledge, these two immobilization materials were used for the first time for this purpose. Two different bioreactor configurations were compared for the performance parameters such as methane formation rate, H-2 consumption and methane contents in the headspace. Methane content in the headspace of these bioreactors were measured to be 80 and 75% for MBBR bioreactor and glass bead bioreactors, respectively. in addition, methane formation rates (MFR) of 5.14 and 4.8 m(3)/m(3)/day were achieved in MBBR and glass beads bioreactors, respectively. Even though both bioreactor configurations performed highly efficient biomethanation of CO2, the statistical evaluation of the results indicated that MBBR performance was more favourable for hydrogenotrophic biomethanation. (c) 2021 Society of Chemical Industry and John Wiley & Sons, Ltd.Ege University Scientific Research Project FundEge University [17/CSUAM/002]The authors thank Ege University Scientific Research Project Fund for the financial support of this study under the Grant No 17/CSUAM/002 The authors also thank Aquaflex Company (Turkey) for the support of supplying bioaqua MBBR rings