Prevalensi Labioschisis Di Rsup. Prof. Dr. R. D. Kandou Manado Periode Januari 2011 – Oktober 2012

: Cleft lip or labioschisis is an inherited disorder that can occur on the lips to the ceiling. Cleft lip is a disruption in the face of growth since the fourth week of embryonic life. Method: This research in retrospection description research for knowning prevalence cleft lip or labioschisis in surgical department RSUP. Prof. Dr. R. D. Kandou Manado, period of Januari 2011 – October 2012. Output: Prevalence of Labioschisis and Labiopalatochisis on Januari 2011 – October 2012 is 57% and 43%. Presentation for each of kind harelipped are : unilateral labioschisis 47%, bilateral labioschisis 5%, unilateral palatum of labioshisis 28%, and bilateral palatum of labioschisis 12%, submucosa 1%, and cleft palate lips 7%. Presentation according to the place of defect : right 18%, left 57%, bilateral 25%, and status not complete 54%. Presentation according to age for doing operation : 0-4 years 73%, 5-9 years 10%, 10-14 years 7%, and >15 years 10%. Presentation labioschisis according to sex : Man 58%, and women 42%. Presentation labioschisis according to etiology : genetic factor 25%, environment factor 62%, and unknown factor 13%. Presentation of labioschisis that be surgery 93%, and not surgery 7%. Presentation of labioschisis according to complication surgery : bleeding post surgery 1%, secunder infection 4%, dehisensi/establish scar 4%, and not complication 91%. Conclusion: Prevalence labioschisis still decrease in each year, kind of labioschisis that large is unilateral labioschisis and localization defect is often on left edge. Labioschisis is happen more to man. Factor that to cause labioschisis between : genetic factor, environment factor and unknown factor. Labioschisis is often more to surgery 0-4 years old

Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation.

Neural stem cell (NSC) transplantation can influence immune responses and suppress inflammation in the CNS. Metabolites, such as succinate, modulate the phenotype and function of immune cells, but whether and how NSCs are also activated by such immunometabolites to control immunoreactivity and inflammatory responses is unclear. Here, we show that transplanted somatic and directly induced NSCs ameliorate chronic CNS inflammation by reducing succinate levels in the cerebrospinal fluid, thereby decreasing mononuclear phagocyte (MP) infiltration and secondary CNS damage. Inflammatory MPs release succinate, which activates succinate receptor 1 (SUCNR1)/GPR91 on NSCs, leading them to secrete prostaglandin E2 and scavenge extracellular succinate with consequential anti-inflammatory effects. Thus, our work reveals an unexpected role for the succinate-SUCNR1 axis in somatic and directly induced NSCs, which controls the response of stem cells to inflammatory metabolic signals released by type 1 MPs in the chronically inflamed brain

Bonding of the Inner Tracker Silicon Microstrip Modules

Microbonding of the CMS Tracker Inner Barrel (TIB) and Tracker Inner Disks (TID) modules was shared among six different Italian Institutes. The organization devised and the infrastructure deployed to handle this task is illustrated. Microbonding specifications and procedures for the different types of TIB and TID modules are given. The tooling specially designed and developed for these types of modules is described. Experience of production is presented. Attained production rates are given. An analysis of the microbonding quality achieved is presented, based on bond strengths measured in sample bond pull tests as well as on rates of bonding failures. Italian Bonding Centers routinely performed well above minimum specifications and a very low global introduced failure rate, at the strip level, of only $\sim$0.015 \% is observed

Strutture e funzioni di una piattaforma online per gli attori della sicurezza

La presente pubblicazione riassume i risultati emersi nell’ambito del progetto di ricerca ID 19 “RLS OnLine e Picasso: la Rete per il Lavoro Sicuro italiano. Apertura al pubblico nazionale degli RLS della piattaforma informatica collaborativa per la salute e sicurezza sul lavoro per la generazione e lo scambio di nuove pratiche in materia di SSL, con l’apporto delle confederazioni comparativamente più rappresentative” finanziato dall’INAIL (Bando Bric 2019) e realizzato dall’Università degli Studi di Perugia, dall’Università degli Studi di Cagliari, dalla Fondazione Giuseppe Di Vittorio e da IAL Nazionale - Innovazione Apprendimento Lavoro s.r.l., con la partecipazione delle Organizzazioni Sindacali. Il lavoro, frutto del lavoro condiviso dell’ente finanziatore INAIL, della Fondazione Giuseppe Di Vittorio, di IAL Nazionale - Innovazione Apprendimento Lavoro s.r.l., di CGIL - Confederazione Generale Italiana del Lavoro, di CISL - Confederazione Italiana Sindacati Lavoratori, di UIL - Unione Italiana del Lavoro, e del Dipartimento di Giurisprudenza dell’Università degli Studi di Perugia, illustra la metodologia utilizzata nel corso della ricerca nonché i risultati emersi dalla stessa con particolare attenzione alle nuove esigenze degli attori della prevenzione

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Extracellular vesicles are independent metabolic units with asparaginase activity.

Extracellular vesicles (EVs) are membrane particles involved in the exchange of a broad range of bioactive molecules between cells and the microenvironment. Although it has been shown that cells can traffic metabolic enzymes via EVs, much remains to be elucidated with regard to their intrinsic metabolic activity. Accordingly, herein we assessed the ability of neural stem/progenitor cell (NSC)-derived EVs to consume and produce metabolites. Our metabolomics and functional analyses both revealed that EVs harbor L-asparaginase activity, catalyzed by the enzyme asparaginase-like protein 1 (Asrgl1). Critically, we show that Asrgl1 activity is selective for asparagine and is devoid of glutaminase activity. We found that mouse and human NSC EVs traffic Asrgl1. Our results demonstrate, for the first time, that NSC EVs function as independent metabolic units that are able to modify the concentrations of critical nutrients, with the potential to affect the physiology of their microenvironment.This work has received support from the Italian Multiple Sclerosis Association (AISM, grant 2010/R/31 and grant 2014/PMS/4 to SP), the Italian Ministry of Health (GR08-7 to SP), the European Research Council (ERC) under the ERC-2010-StG Grant agreement n° 260511-SEM_SEM, the Medical Research Council, the Engineering and Physical Sciences Research Council, and the Biotechnology and Biological Sciences Research Council UK Regenerative Medicine Platform Hub “Acellular Approaches for Therapeutic Delivery” (MR/K026682/1 to SP), The Evelyn Trust (RG 69865 to SP), The Bascule Charitable Trust (RG 75149 to SP) and core support grant from the Wellcome Trust and Medical Research Council to the Wellcome Trust – MRC Cambridge Stem Cell Institute. N.I. was supported by a FEBS long-term fellowship. C.F., A.S.H., and E.G. were funded by the Medical Research Council, Core Fund SKAG006

Unraveling Crimes with Geology: As Geological and Geographical Evidence Related to Clandestine Graves May Assist the Judicial System

The geological and geographical evidence related to crime scenes involving clandestine graves is valuable data to consider during judicial investigations because it can provide useful criminological and criminalistic information. Research results on the nature and main features of historical cases and thirty criminal burials are reported. Among the studied cases, a recent homicide clandestine grave was analyzed through remote sensing. This latter allowed the definition of GIS-based RAG maps and search priority scenarios and ascertain that the study grave fell in a high priority Red coded area, validating a method previously based only on simulated crime scenes

Unraveling Crimes with Geology: As Geological and Geographical Evidence Related to Clandestine Graves May Assist the Judicial System

The geological and geographical evidence related to crime scenes involving clandestine graves is valuable data to consider during judicial investigations because it can provide useful criminological and criminalistic information. Research results on the nature and main features of historical cases and thirty criminal burials are reported. Among the studied cases, a recent homicide clandestine grave was analyzed through remote sensing. This latter allowed the definition of GIS-based RAG maps and search priority scenarios and ascertain that the study grave fell in a high priority Red coded area, validating a method previously based only on simulated crime scenes

GIS-based RAG-coded search priority scenarios for predictive maps to prevent future serial serious crimes: The case study of the Florence Monster

In the present research, the GIS (Geographical Information System)-based RAG (Red-Amber-Green) color-coded search priority system was applied to the case study of the serial murders of the monster of Florence occurred between 1968 and 1985 in the Florence province (northern Italy). Through a predictive search priority scenario, the RAG maps were aimed to identify the possible areas where the monster could have hypothetically continued to hit if still alive. The identified Red-code area allowed to obtain a very useful result, reducing to the 21% the extension of the site where the serial killer had to continue to hit. In predictive terms, this method, if applied at that epoch, could have helped law enforcements and the judicial authority to strongly reduce the areas to monitor for arresting the responsible of these tremendous murderers