4 research outputs found
Role Of Inos-no-cgmp Signaling In Modulation Of Inflammatory And Myelination Processes
Nitric oxide (NO) is the main activator of the soluble guanylate cyclase (sGC)-guanosine 3'5' cyclic monophosphate (cGMP) pathway. The level of cGMP is regulated by phosphodiesterases (PDEs), which break down cGMP. It has been reported that levels of NO in the central nervous system (CNS) can greatly increase during demyelination and/or neuroinflammation. Controversially, in demyelination models, mice without iNOS may develop more severe cases of disease. Furthermore, cGMP accumulation caused by PDE inhibitors has an anti-inflammatory/neuroprotective effect in MS-models. The role of the NO-cGMP pathway in the nervous tissue is, therefore, complex and not fully understood. The aim of the present study was to contribute to existing knowledge of the role of this pathway in the CNS. Wild type (WT - C57BL/6) and iNOS-/- animals were treated with sildenafil (25mg/kg) for 8 weeks. Control animals were not treated. VCAM and ICAM (adhesion proteins), GFAP and Iba-1 (astrocyte and microglia markers, respectively), PKG (cGMP-dependent protein kinase), sGC, eNOS (constitutive endothelial NO sinthase) and GSTpi (a marker of mature oligodendrocytes) were evaluated in the cerebellum using immunohistochemistry or western blotting. Myelin was assessed by luxol fast blue staining and electron transmission microscopy. Treatment with sildenafil reduced ICAM and VCAM levels (anti-inflammatory effect) and increased GFAP and Iba-1 expression (clearance phenotype) in WT animals. The expression of VCAM, ICAM, GFAP, PKG and sGC was lower in iNOS-/- mice than in WT control animals. The treatment of iNOS-/- animals with sildenafil resulted in an increase of all proteins (pro-inflammatory effect). There was overexpression of eNOS in untreated iNOS-/- mice. The myelin structure of iNOS-/- animals was damaged in comparison with WT control. Sildenafil increased GSTpi and resulted in an improved myelin structure in iNOS-/- mice. In conclusion, NO-cGMP signaling plays a role in the regulation of inflammation and myelination processes. The accumulation of cGMP produced opposite effects in WT and iNOS-/- mice. This can be explained by the overexpression of eNOS in iNOS-/- mice, unbalancing cGMP signaling, or cGMP has a dual role in inflammation. Drugs that modulate the NO-sGC-cGMP pathway may be clinically beneficial in the treatment of neuroinflammatory/demyelinating disorders, but further studies of the regulation of this pathway are required. © 2014 Elsevier Inc.1046073Agullo, L., Baltrons, M.A., Garcia, A., Calcium-dependent nitric oxide formation in glial cells (1995) Brain Res., 686, pp. 160-168Arnett, H.A., Hellendall, R.P., Matsushima, G.K., Suzuki, K., Laubach, V.E., Sherman, P., Ting, J.P., The protective role of nitric oxide in a neurotoxicant-induced demyelinating model (2002) J. 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Efeito da temperatura de descongelação na integridade de espermatozoides criopreservados de cães
Avaliou-se a influência da temperatura de descongelação na integridade de espermatozoides criopreservados de cães. Foram utilizados reprodutores das raças Basset Hound (n=3) e Rottweiler (n=3), submetidos a colheitas de sêmen por manipulação peniana. As amostras de sêmen foram descongeladas a 37ºC/1min (G1) ou 70ºC/6s (G2) e avaliadas quanto à motilidade progressiva, vigor e integridade do acrossoma após 0, 30 e 60 minutos de incubação (37ºC), e ultraestrutura espermática imediatamente após a descongelação. Em todos os tempos de incubação, a motilidade progressiva dos espermatozoides descongelados a 70ºC por 6s (74,6%) foi mais alta (P0,05) entre os grupos, e o porcentual de gametas com acrossomas íntegros foi maior (P<0,05) nos espermatozoides do G1 do que no G2. Lesões ultraestruturais foram identificadas nos espermatozoides descongelados de ambos os grupos, em maior quantidade nos gametas do G2. Conclui-se que amostras congeladas de sêmen de cães devam ser descongeladas a 37ºC por 1min
Tipo de conhecimento sobre inclusão produzido pelas pesquisas Type of knowledge about inclusion produced by research
As pesquisas brasileiras sobre produção de conhecimento no campo da Educação Especial iniciaram-se há mais de 15 anos. No presente objetivou-se analisar o tipo de conhecimento produzido nessa área frente à metodologia empregada nas dissertações e teses financiadas pelo Programa de Apoio a Educação Especial. Os trabalhos de conclusão de curso analisados referiam-se àqueles projetos aprovados pelo Proesp no âmbito do Estado de São Paulo. O período de análise ficou compreendido entre 2004 e 2008. Vinte e sete estudos foram localizados. A análise dos estudos foi realizada por meio de cinco categorias: 1) pesquisas que apresentaram generalização e aplicação imediata dos resultados; 2) pesquisas que apresentaram resultados imediatos para um grupo específico de participantes; 3) pesquisas descritivas com achados inovadores; 4) pesquisas de intervenção com achados inovadores; 5) pesquisas descritivas que corroboraram outras pesquisas. Foi possível concluir que os estudos avançaram nos conhecimentos sobre a inclusão e que o uso de metodologias pouco utilizadas no campo da educação serviu de subsídio para obter os resultados.<br>The Brazilian research on knowledge production in the field of Special Education began more than 15 years ago. The objective was to analyze the type of knowledge produced in the area before the methodology used in dissertations funded by the "Programa de Apoio a Educação Especial - PROESP". The final paper analyzed referred to those projects approved by PROESP within the State of São Paulo. The analysis period was between 2004 and 2008. Twenty-seven studies were found. The review was conducted through five categories: 1) studies that showed generalization and immediate application of the results, 2) research that showed immediate results for a specific group of participants, 3) descriptive research with innovative findings, 4) intervention research with innovative findings and 5) descriptive research that corroborated other studies. It was concluded that the studies advance in knowledge on inclusion and the use of some infrequent methodologies in education served as a subsidy to get the results