System and method for tuning adjusting the central frequency of a laser while maintaining frequency stabilization to an external reference

A method and system for stabilizing a laser to a frequency reference with an adjustable offset. The method locks a sideband signal generated by passing an incoming laser beam through the phase modulator to a frequency reference, and adjusts a carrier frequency relative to the locked sideband signal by changing a phase modulation frequency input to the phase modulator. The sideband signal can be a single sideband (SSB), dual sideband (DSB), or an electronic sideband (ESB) signal. Two separate electro-optic modulators can produce the DSB signal. The two electro-optic modulators can be a broadband modulator and a resonant modulator. With a DSB signal, the method can introduce two sinusoidal phase modulations at the phase modulator. With ESB signals, the method can further drive the optical phase modulator with an electrical signal with nominal frequency OMEGA(sub 1) that is phase modulated at a frequency OMEGA(sub 2

Photoperiodic diapause under the control of circadian clock genes in an insect

<p>Abstract</p> <p>Background</p> <p>Most organisms have evolved a circadian clock in order to anticipate daily environmental changes and many of these organisms are also capable of sophisticated measurement of daylength (photoperiodism) that is used to regulate seasonal events such as diapause, migration and polymorphism. It has been generally accepted that the same elements are involved in both circadian (daily) and seasonal (annual) rhythms because both rely upon daily light-dark cycles. However, as reasonable as this sounds, there remains no conclusive evidence of such a molecular machinery in insects. We have approached this issue by using RNA interference (RNAi) in <it>Riptortus pedestris</it>.</p> <p>Results</p> <p>The cuticle deposition rhythm exhibited the major properties of circadian rhythms, indicating that the rhythm is regulated by a circadian clock. RNAi directed against the circadian clock genes of <it>period </it>and <it>cycle</it>, which are negative and positive regulators in the circadian clock, respectively, disrupted the cuticle deposition rhythm and distinct cuticle layers were produced by these RNAi. Simultaneously, <it>period </it>RNAi caused the insect to avert diapause under a diapause-inducing photoperiod whereas <it>cycle </it>RNAi induced diapause under a diapause-averting photoperiod. The expression patterns of juvenile hormone-regulated genes and the application of juvenile hormone analogue suggested that neither ovarian development itself nor a downstream cascade of juvenile hormone secretion, were disturbed by <it>period </it>and <it>cycle </it>RNAi.</p> <p>Conclusions</p> <p>This study revealed that the circadian clock genes are crucial not only for daily rhythms but also for photoperiodic diapause. RNAi directed against <it>period </it>and <it>cycle </it>had opposite effects not only in the circadian cuticle deposition rhythm but also in the photoperiodic diapause. These RNAi also had opposite effects on juvenile hormone-regulated gene expression. It is still possible that the circadian clock genes pleiotropically affect ovarian development but, based on these results, we suggest that the circadian clock operated by the circadian clock genes, <it>period </it>and <it>cycle</it>, governs seasonal timing as well as the daily rhythms.</p> <p>See Commentary: <url>http://www.biomedcentral.com/1741-7007/8/115</url></p

Fire and edge effects in a fragmented tropical forest landscape in the southwestern Amazon.

The Amazon holds the largest tropical rain forest formation in the world but this natural ecosystem has been altered by both anthropogenic and natural disturbances since the 1970s (Davidson et al., 2012). The Brazilian Amazon experienced the highest annual tropical deforestation rates until the mid-2000s when rates began to decline dramatically due to the government?s environmental law enforcement. Conversely, other forest disturbances, such as understory fire, selective logging, and fragmentation (Aragao et al., 2014; Arima et al., 2014) have gained more importance in terms of their impacts on remnant forests. The degree of forest degradation varies as functions of disturbance type, the intensity and frequency of disturbance events, and the time since occurrence (Cochrane and Schulze, 1999; Barlow and Peres, 2004; Brando et al., 2014). Additionally, the impacts of these disturbances may vary across the region due to different gradients of physical conditions including rainfall, edaphic and geological properties (Hoorn et al., 2010; Malhi et al., 2004)

Frequency-tunable Pre-stabilized Lasers for LISA via Sideband-locking

Laser frequency noise mitigation is one of the most challenging aspects of the LISA interferometric measurement system. The unstabilized frequency fluctuations must be suppressed by roughly twelve orders of magnitude in order to achieve stability sufficient for gravitational wave detection. This enormous suppression will be achieved through a combination of stabilization and common-mode rejection. The stabilization component will itself be achieved in two stages: pre-stabilization to a local optical cavity followed by arm-locking to some combination of the inter-spacecraft distances. In order for these two stabilization stages to work simultaneously, the lock-point of the pre-stabilization loop must be frequency tunable. The current baseline stabilization technique, locking to an optical cavity, does not provide tunability between cavity resonances, which are typically spaced by 100s of MHz. Here we present a modification to the traditional Pound-Drever-Hall cavity locking technique that allows the laser to be locked to a cavity resonance with an adjustable frequency offset. This technique requires no modifications to the optical cavity itself, thus preserving the stability of the frequency reference. We present measurements of the system performance and demonstrate that we can meet implement the first two stages of stabilization

The FEZ1 gene at chromosome 8p22 encodes a leucine-zipper protein, and its expression is altered in multiple human tumors

Alterations of human chromosome 8p occur frequently in many tumors. We identified a 1.5-Mb common region of allelic loss on 8p22 by allelotype analysis, cDNA selection allowed isolation of several genes, including FEZ1. The predicted Fez1 protein contained a leucine-zipper region with similarity to the DNA-binding domain of the cAMP-responsive activating-transcription factor 5. RNA blot analysis revealed that FEZ1 gene expression was undetectable in more than 60% of epithelial tumors. Mutations were found in primary esophageal cancers and in a prostate cancer cell line. Transcript analysis from several FEZ1-expressing tumors revealed truncated mRNAs, including a frameshift. Alteration and inactivation of the FEZ1 gene may play a role in various human tumors

The trail of my studies on glycoproteins from enterokinase to tumor markers

This review describes the results of the author’s studies on glycoproteins which have been carried out for more than 50 years. Starting from the elucidation of basic structures of glycoproteins, i.e. the structure of the linkage between an amino acid and a sugar and the occurrence of the β-mannosidic linkage as the common structure of glycoproteins, the author became interested in the cell membrane glycoproteins focused on the comparison of cancer cells versus normal cells. These studies were then extended to the establishment of sugar-directed and cancer-associated monoclonal antibodies. Some of the monoclonal antibodies are useful for cancer diagnosis