Breast Cancer Classification Using Deep Convolutional Neural Networks

Breast cancer remains the primary causes of death for women and much effort has been depleted in the form of screening series for prevention. Given the exponential growth in the number of mammograms collected, computer-assisted diagnosis has become a necessity. Histopathological imaging is one of the methods for cancer diagnosis where Pathologists examine tissue cells under different microscopic standards but disagree on the final decision. In this context, the use of automatic image processing techniques resulting from deep learning denotes a promising avenue for assisting in the diagnosis of breast cancer. In this paper, an android software for breast cancer classification using deep learning approach based on a Convolutional Neural Network (CNN) was developed. The software aims to classify the breast tumors to benign or malignant. Experimental results on histopathological images using the BreakHis dataset shows that the DenseNet CNN model achieved high processing performances with 96% of accuracy in the breast cancer classification task when compared with state-of-the-art models

Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography

Background: Maintenance of the ratio of glutathione in the reduced (GSH) and oxidised (GSSG) state in cells is important in redox control, signal transduction and gene regulation, factors that are altered in many diseases. The accurate and reliable determination of GSH and GSSG simultaneously is a useful tool for oxidative stress determination. Measurement is limited primarily to the underestimation of GSH and overestimation GSSG as a result of auto-oxidation of GSH. The aim of this study was to overcome this limitation and develop, optimise and validate a reverse-phase high performance liquid chromatographic (HPLC) assay of GSH and GSSG for the determination of oxidant status in cardiac and chronic kidney diseases. Methods: Fluorescence detection of the derivative, glutathione-O-pthaldialdehyde (OPA) adduct was used. The assay was validated by measuring the stability of glutathione and glutathione-OPA adduct under conditions that could affect the reproducibility including reaction time and temperature. Linearity, concentration range, limit of detection (LOD), limit of quantification (LOQ), recovery and extraction efficiency and selectivity of the method were assessed. Results: There was excellent linearity for GSH (r2 = 0.998) and GSSG (r2 = 0.996) over concentration ranges of 0.1 μM–4 mM and 0.2 μM–0.4 mM respectively. The extraction of GSH from tissues was consistent and precise. The limit of detection for GSH and GSSG were 0.34 μM and 0.26 μM respectively whilst their limits of quantification were 1.14 μM and 0.88 μM respectively. Conclusion: These data validate a method for the simultaneous measurement of GSH and GSSG in samples extracted from biological tissues and offer a simple determination of redox status in clinical samples

Prevalence of low back pain and associated factors among office workers in Kano city, Nigeria

Background: Many studies across the globe have reported the prevalence of low back pain (LBP) among office workers. This study aimed to find out the prevalence of LBP and associated factors among office workers in Kano city, Nigeria.Methods: A cross-sectional type of study was conducted among the office workers in Kano metropolitan from September to December 2019. A total of 300 office workers were selected using a convenience sampling technique. The data was collected from the respondents by face-to-face interview technique using a semi-structured questionnaire and all the data collected were analyzed using statistical software (SPSS version 22.0).Results: In this study, the mean age of the respondents was 38.6±9.6 years and 72.7% of them were male. More than half (54.7%) of the respondents had a bachelor's degree and above. About 71.3% of the respondents had a familial history of LBP. About 68.0% of the respondents had suffered musculoskeletal disorder in the past and the majority (54.4%) mentioned they suffered hip pain. Only 33.7% of the respondents were maintaining the proper posture and 37.7% of the respondents were using an ergonomic chair.Conclusions: The findings of this study found a 65.3% prevalence of low back pain among the office workers in Kano metropolitan. Office workers should be encouraged to maintain proper postures at work and there is a need for more educational programs regarding the prevention measures of low back pain

SPARC 2019 Fake news & home truths : Salford postgraduate annual research conference book of abstracts

Welcome to the Book of Abstracts for the 2019 SPARC conference. This year we not only celebrate the work of our PGRs but also our first ever Doctoral School Best Supervisor awards, which makes this year’s conference extra special. Once again we have received a tremendous contribution from our postgraduate research community; with over 90 presenters, the conference truly showcases a vibrant, innovative and collaborative PGR community at Salford. These abstracts provide a taster of the inspiring, relevant and impactful research in progress, and provide delegates with a reference point for networking and initiating critical debate. Find an abstract that interests you, and say “Hello” to the author. Who knows what might result from your conversation? With such wide-ranging topics being showcased, we encourage you to take up this great opportunity to engage with researchers working in different subject areas from your own. To meet global challenges, high impact research needs interdisciplinary collaboration. This is recognised and rewarded by all major research funders. Engaging with the work of others and forging collaborations across subject areas is an essential skill for the next generation of researchers. Even better, our free ice cream van means that you can have those conversations while enjoying a refreshing ice lolly

Oxidative Stress and Cardiovascular Complications in Chronic Kidney Disease, the Impact of Anaemia

Patients with chronic kidney disease (CKD) have significant cardiovascular morbidity and mortality as a result of risk factors such as left ventricular hypertrophy (LVH), oxidative stress, and inflammation. The presence of anaemia in CKD further increases the risk of LVH and oxidative stress, thereby magnifying the deleterious consequence in uraemic cardiomyopathy (UCM), and aggravating progression to failure and increasing the risk of sudden cardiac death. This short review highlights the specific cardio-renal oxidative stress in CKD and provides an understanding of the pathophysiology and impact of uraemic toxins, inflammation, and anaemia on oxidative stress

Oxidative Stress and Cardiovascular Complications in Chronic Kidney Disease, the Impact of Anaemia

Patients with chronic kidney disease (CKD) have significant cardiovascular morbidity and mortality as a result of risk factors such as left ventricular hypertrophy (LVH), oxidative stress, and inflammation. The presence of anaemia in CKD further increases the risk of LVH and oxidative stress, thereby magnifying the deleterious consequence in uraemic cardiomyopathy (UCM), and aggravating progression to failure and increasing the risk of sudden cardiac death. This short review highlights the specific cardio-renal oxidative stress in CKD and provides an understanding of the pathophysiology and impact of uraemic toxins, inflammation, and anaemia on oxidative stress

Impact of Intravenous Iron on Oxidative Stress and Mitochondrial Function in Experimental Chronic Kidney Disease

Background: Mitochondrial dysfunction is observed in chronic kidney disease (CKD). Iron deficiency anaemia (IDA), a common complication in CKD, is associated with poor clinical outcomes affecting mitochondrial function and exacerbating oxidative stress. Intravenous (iv) iron, that is used to treat anaemia, may lead to acute systemic oxidative stress. This study evaluated the impact of iv iron on mitochondrial function and oxidative stress. Methods: Uraemia was induced surgically in male Sprague-Dawley rats and studies were carried out 12 weeks later in two groups sham operated and uraemic (5/6 nephrectomy) rats not exposed to i.v. iron versus sham operated and uraemic rats with iv iron. Results: Induction of uraemia resulted in reduced iron availability (serum iron: 31.1 ± 1.8 versus 46.4 ± 1.4 µM), low total iron binding capacity (26.4 ± 0.7 versus 29.5 ± 0.8 µM), anaemia (haematocrit: 42.5 ± 3.0 versus 55.0 ± 3.0%), cardiac hypertrophy, reduced systemic glutathione peroxidase activity (1.12 ± 0.11 versus 1.48 ± 0.12 U/mL), tissue oxidative stress (oxidised glutathione: 0.50 ± 0.03 versus 0.36 ± 0.04 nmol/mg of tissue), renal mitochondrial dysfunction (proton/electron leak: 61.8 ± 8.0 versus 22.7 ± 5.77) and complex I respiration (134.6 ± 31.4 versus 267.6 ± 26.4 pmol/min/µg). Iron therapy had no effect on renal function and cardiac hypertrophy but improved anaemia and systemic glutathione peroxidase (GPx) activity. There was increased renal iron content and complex II and complex IV dysfunction. Conclusion: Iron therapy improved iron deficiency anaemia in CKD without significant impact on renal function or oxidant status

The Nigerian Petroleum Industry Act, Frontier Basins Exploration and the Global Energy Transition

The Nigerian Petroleum Industry Act (PIA) passed into Law in 2021 has the major goal to reform the Nigerian petroleum sector operations into policy, regulations and business (commercial). In the line of this, the Nigerian National Petroleum Corporation (NNPC) was transformed to NNPC Ltd to operate entirely commercially with a supervising Board and registration with the Corporate Affairs Commission. Such a commercial mandate will entail the need to explore and produce more oil and gas for export and domestic utilization. Oil is becoming less attractive as an energy source but gas is gaining momentum as a clean energy source in the global energy transition road-map. The global energy transition road-map is drawn around clean, alternative and renewable energies. The Nigerian frontier basins have recently come on board as new business opportunities with huge petroleum gas resources. These frontier basins comprise the Anambra, Benue, Bida, Chad (Nigerian sector), Dahomey, and Sokoto Basins as well as the Deep and Ultra-deep offshore. Maturing these basins through data generation and production of the gas resources therein will promote the nation’s gas utilization and gas expansion programmes meant to promote industrialization and huge employment generation, grow the economy and engender positive social transformation. The clause in the PIA that promotes frontier exploration is well-thought out. Available and required geological data needed to mature the frontier basins to producing basins are presented in this paper. The success made in Kolmani River-2 well discovery is a case study

Adaptation of the Wound Healing Questionnaire universal-reporter outcome measure for use in global surgery trials (TALON-1 study): mixed-methods study and Rasch analysis

BackgroundThe Bluebelle Wound Healing Questionnaire (WHQ) is a universal-reporter outcome measure developed in the UK for remote detection of surgical-site infection after abdominal surgery. This study aimed to explore cross-cultural equivalence, acceptability, and content validity of the WHQ for use across low- and middle-income countries, and to make recommendations for its adaptation.MethodsThis was a mixed-methods study within a trial (SWAT) embedded in an international randomized trial, conducted according to best practice guidelines, and co-produced with community and patient partners (TALON-1). Structured interviews and focus groups were used to gather data regarding cross-cultural, cross-contextual equivalence of the individual items and scale, and conduct a translatability assessment. Translation was completed into five languages in accordance with Mapi recommendations. Next, data from a prospective cohort (SWAT) were interpreted using Rasch analysis to explore scaling and measurement properties of the WHQ. Finally, qualitative and quantitative data were triangulated using a modified, exploratory, instrumental design model.ResultsIn the qualitative phase, 10 structured interviews and six focus groups took place with a total of 47 investigators across six countries. Themes related to comprehension, response mapping, retrieval, and judgement were identified with rich cross-cultural insights. In the quantitative phase, an exploratory Rasch model was fitted to data from 537 patients (369 excluding extremes). Owing to the number of extreme (floor) values, the overall level of power was low. The single WHQ scale satisfied tests of unidimensionality, indicating validity of the ordinal total WHQ score. There was significant overall model misfit of five items (5, 9, 14, 15, 16) and local dependency in 11 item pairs. The person separation index was estimated as 0.48 suggesting weak discrimination between classes, whereas Cronbach's α was high at 0.86. Triangulation of qualitative data with the Rasch analysis supported recommendations for cross-cultural adaptation of the WHQ items 1 (redness), 3 (clear fluid), 7 (deep wound opening), 10 (pain), 11 (fever), 15 (antibiotics), 16 (debridement), 18 (drainage), and 19 (reoperation). Changes to three item response categories (1, not at all; 2, a little; 3, a lot) were adopted for symptom items 1 to 10, and two categories (0, no; 1, yes) for item 11 (fever).ConclusionThis study made recommendations for cross-cultural adaptation of the WHQ for use in global surgical research and practice, using co-produced mixed-methods data from three continents. Translations are now available for implementation into remote wound assessment pathways