Efek Pemberian Pupuk Hayati VP3 yang Diperkaya Trichoderma viride FRP3 terhadap Total Populasi Mikroorganisme Tanah dan Pertumbuhan Tanaman Kedelai (Glycine max (L) Merrill)

Biofertilizer is a living microorganism material and is useful for increasing soil fertility and the production quality of a plant. The VP3 biofertilizer formulation is known to increase the activity of beneficial microorganisms for plant growth. Trichoderma viride is a good microorganism used as a biopesticide. This study was conducted to determine the effect of VP3 and Trichoderma viride FRP3 biofertilizers on the growth of soybean (Glycine max (L) Merrill) and the total population of soil microorganisms. This research was conducted in the greenhouse of the Faculty of Agriculture and the Halal Center Intedrated Laboratory of Microbiology, Islamic University of Malang. This study used a simple Randomized Block Design (RAK) with 7 treatments with 3 replications. The results of the data analysis using the analysis of variance with the F test with a significance level of 5%, if there was a significant effect between treatments, it was continued with the BNT 5% if there was a significant effect. The results showed that treatment N had the highest average yield for soybean plant growth

Effect of VP3 Biological Fertilizer Enriched with Trichoderma viride FRP3 on Growth and Chlorophyll Content in Soybean Leaves (Glycine max. L)

Biofertilizer is a living microorganism material that is useful for increasing soil fertility and the quality of a plant's production. VP3 biofertilizer is a liquid biofertilizer formulation made from vermiwash as a carrier, molasses, PEG, and 3 functional bacterial isolates. In this study, the VP3 biofertilizer will be enriched with Trichoderma viride FRP3. This study aims to determine the effect of giving VP3 biofertilizer enriched with Trichoderma viride FRP3 on yield and chlorophyll content in Soybean (Glycine max) leaves. This study was an experiment in polybags conducted at the Greenhouse using a Randomized Block Design consisting of 7 treatments, each treatment repeated 3 times and each replication using 4 plant samples. The variables observed included growth variables consisting of plant height, number of leaves and leaf area and leaf chlorophyll content. Data from observations on each plant parameter were then tested using analysis of variance F test with a level 5% significant, if there is a significant effect between treatments followed by Duncan's test at 5% level.The results showed that the application of VP3 biofertilizer enriched with Trichoderma viride FRP3 had no significant effect on plant growth parameters, because in general the treatment of N (m (using NPK fertilizer) showed high yields compared to other treatments, but the application of VP3 biological fertilizer enriched with Trichoderma viride FRP3 in the chlorophyll content of soybean leaves was able to compensate for the application of inorganic NPK fertilizer

Efek Pemberian Pupuk Hayati VP3 yang Diperkaya Trichoderma viride FRP3 terhadap Pertumbuhan Fase Generatif Tanaman Kedelai (Glycine max L.)

VP3 biofertilizer is a fertilizer composed of vermiwash, molasses and 1% PEG, with 3 bacterial isolates added. Trichoderma viride FRP3 is a bioremediation agent that can degrade land contaminated with the herbicide glyphosphate. The purpose of this study was to determine the effect of the application of VP3 biofertilizer enriched with Trichoderma viride FRP3 on the growth of the generative phase of soybean (Glycine max L.) grown in greenhouses. The research was conducted in the greenhouse of the Faculty of Agriculture, and Laboratory of Microbiology, University of Islam Malang from November 2021 to February 2022. The research design used a Simple Randomized Block Design (RBD) with 7 treatments and was repeated 3 times. The results of the study showed that treatment N (NPK fertilizer) gave the highest average yield on observations of plant height, number of leaves, leaf area, total flowers, and total pods. The best result on the parameter of the percentage of flowers into pods was in treatment V2 (compost fertilizer + manure + VP3 biofertilizer 3 times application) which was 31,74%. Keywords : VP3 Biofertilizer, Trichoderma viride FRP3, Soybean

Efektivitas Aplikasi Beberapa Macam Pupuk Organik Dibandingkan Dengan Pupuk Anorganik Terhadap Pertumbuhan dan Kandungan Klorofil Tanaman Kailan (Brassica oleraceae var. alboglabra)

Kailan cultivation is generally done by farmers using inorganic fertilizers. This kind of farming system in the long term is detrimental to farmers because the soil will become infertile, the quantity and quality of vegetables will decrease. This study aims to compare the effect of using several kinds of organic fertilizers compared to inorganic fertilizers on the growth of kailan plants. This study used a Simple Randomized Block Design (RBD). There were 7 kinds of treatments, including: P0 = No fertilizer application, P1 = NPK fertilizer 4.5 g/pot, P2 = Compost fertilizer 218 g/pot, P3 = Cow dung fertilizer 397 g/pot, P4 = Chicken manure fertilizer 111 g /pot, P5 = Vermicompost fertilizer 511 g/pot, and P6 = Vermicompost powder fertilizer 147 g/pot. The dose of organic fertilizer applied is equivalent to the N content in NPK fertilizer and converted to the water content of the organic fertilizer. The variables observed included: plant height, number of leaves, leaf area and chlorophyll content. The data collected were analyzed for variance (F test) with a level of 5% to determine the effect of treatment. If the results of the F test have a significant effect, then continue with the further LSD test with a level of 5% to determine the effect of growth on plant yields. The results of this study indicate that at the end of the observation of plant growth (plant height and leaf area) the best was found in the vermicompost treatment, while the number of leaves of inorganic fertilizer, compost, vermicompost and vermicompost powder gave the same number of leaves. However, when viewed from the level of green leaf color (chlorophyll content) as measured by using a SPAD meter, vermicompost powder gave the highest chlorophyll content. Keywords : Kailan, Fertilizer, Growth respons

Pengaruh Suhu Pengeringan pada Viabilitas Agen Hayati Pelet BioferNA Berbahan Dasar Limbah Rumput Laut dan Pengaruhnya pada 2 Bibit Tanaman

The seaweed industry's solid waste has the potential to be converted into organic fertilizer. The biological fertilizer used in this study was VP3 biological fertilizer, which is a liquid formulation fertilizer made from 3 functional bacterial isolates, molasses, and PEG 1% and vermiwash as a carrier. The goal of this study was to determine the ideal drying temperature for producing BioferNA pellet fertilizer made from waste seaweed. To determine their impact on plant growth, the drying results with the best total viability of biological agents will be tested on tomato and chili plant seeds. The design used in the Biological Agent Viability Test was Completely Randomized Design (CRD) with 10 treatments and was repeated 3 times. The design used in the test on plant seeds used a  Completely Randomized Block Design (RCBD) which consisted of 5 treatments, and was repeated 4 times. All the observed data are analyzed statistically using analysis of variance (ANOVA) 5% significance level, if there is a significant difference betweem the treatments, it will have a further test using different test average with BNT at 5% significance level. The results showed that the drying temperature of BioferNA pellets made from seaweed waste had a significant effect on the total viability of biological agents. The drying temperature of 40°C has a higher average total viability of biological agents than the temperature of 50°C. BioferNA pellets made from seaweed waste, which were added with VP3 and Trichoderma viride FRP3, had a significant effect on seedling height and wet weight of chili and tomato seedlings, but had no significant effect on the root length of tomato seedlings. The observed growth parameters did not significantly affect the V1 (VP3) and V4 (VP3 + T. viride FRP3 + Vacuum) treatments.Keywords : VP3 Biofertilizer, Trichoderma viride FRP3, Seaweed Waste, Tomato, Chil

APLIKASI PUPUK HAYATI VP3 DIBANDINGKAN DENGAN EMPAT MACAM PUPUK HAYATI YANG BEREDAR DIPASARAN TERHADAP HASIL KACANG HIJAU (Vigna radiata L.) DI LAPANG

ABSTRACTThe aims of this research is to determine the effect of application of VP3 biofertilizer compared with biofertilizers circulating in the market on the results of mungbean in field. The research was conducted on land in Bumi Asri Housing, Dau District, Malang Regency, and the Applied Laboratory, Faculty of Agriculture, Malang Islamic University from June to October 2019. The research used a Randomized Block Design (RBD) with 6 treatments and was repeated 3 times. The results showed that the treatment of VP3 biofertilizer when compared with biofertilizers circulating in the market in general gave better results to the growth and yield variables of mungbean in field. This was seen to be especially significant on the growth variable the number of root nodules, and on variable total seeds weight per plot. However, the content of the NPK elements of soil treatment of VP3 biofertilizer and biofertilizers circulating in the market shows the same results, namely the N element in the soil is low, while the P and K elements are high

Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment

Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collectedfrom rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 1011CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus

Isolation and Characteristic of Nitrogen-fixing Bacteria and Phosphate-solubilizing Bacteria From Soil High in Mercury in Tailings and Compost Areas of Artisanal Gold Mine

The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation

Pemanfaatan Eceng Gondok (Eichornia Crassipes) Dan Limbah Pertanian Untuk Persediaan Pakan Ternak Dan Biokompos Berbasis Teknologi Fermentasi

Water hyacinth causes environmental problems in the Selorejo Reservoir due to its rapid growth. This activity aims to introduce fermentation-based technology to farmer groups (klomtan) in the use of water hyacinth as animal feed and compost, reduce environmental impact and increase its economic value. The methods used are (1) Organizing education and training on zero waste product technology through the manufacture of silage and bio compost for water hyacinth weeds; (2) Provide a special place for the manufacture of silage and bio compost; (3) Strengthening klomtan institutions with production and marketing management training; (4) Assist in production monitoring. This program has achieved the following targets: (1) Farmers and ranchers can adopt a zero-waste technology package to increase livestock productivity and agricultural products; (2) Increasing the awareness and ability of klomtan to utilize agricultural waste and water hyacinth weeds; (3) Institutional strengthening of the Karya Makmur and Lestari Jaya Makmur groups so that they can become examples of farming communities in their villages. The results of this activity showed an increase in the knowledge of group members by 85% of the technology offered. Continuous assistance needs to be carried out because fermentation technology is influenced by various factors so that it is not easily applied by klomtan.   Eceng gondok menyebabkan masalah lingkungan di Waduk Selorejo karena pertumbuhannya yang cepat. Tujuan kegiatan ini untuk mengenalkan teknologi berbasis fermentasi kepada kelompok tani (klomtan) dalam pemanfaatan Enceng Gondok sebagai pakan ternak dan kompos, mengurangi dampak lingkungan dan meningkatkan nilai ekonominya. Metode yang digunakan adalah (1) Melakukan penyuluhan dan pelatihan teknologi zero waste product dengan memberikan pelatihan pembuatan silase dan biokompos gulma enceng gondok; (2) Menyediakan tempat khusus pembuatan silase dan biokompos; (3) Penguatan kelembagaan klomtan dengan pelatihan manajemen produksi dan pemasarannya; (4) Melakukan pendampingan monitoring produksi. Kegiatan ini telah mencapai target: (1) Petani dan peternak mampu mengadopsi paket teknologi zero waste product untuk meningkatkan produktivitas ternak dan hasil pertanian; (2) Tumbuhnya kesadaran dan kemampuan klomtan untuk memanfaatkan limbah pertanian dan gulma enceng gondok; (3) Penguatan Manajemen Kelembagaan klomtan Karya Makmur dan Lestari Jaya Makmur agar dapat menjadi contoh masyarakat tani-ternak di desanya. Hasil kegiatan ini menunjukkan peningkatan pengetahuan anggota kelompok sebesar 85% terhadap teknologi yang ditawarkan. Pendampingan secara terus-menerus perlu dilakukan karena teknologi fermentasi dipengaruhi oleh berbagai faktor sehingga tidak mudah diterapkan oleh klomtan