31 research outputs found
Safe and inclusive research practices for qualitative research involving people with dementia:A review of key issues and strategies
A high-resolution autoradiographic apposition method for water-soluble tracers and tissue constituents
The Future of Health Care: Protocol for Measuring the Potential of Task Automation Grounded in the National Health Service Primary Care System
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Specificity mapping of HIV-1 protease by reduced bond inhibitors
An engineered retroviral proteinase from myeloblastosis associated virus acquires pH dependence and substrate specificity of the HIV-1 proteinase
In an attempt to understand the structural reasons for differences in specificity and activity of proteinases from two retroviruses encoded by human immunodeficiency virus (HIV) and myeloblastosis associated virus (MAV), we mutated five key residues predicted to form part of the enzyme subsites S1, S2 and S3 in the substrate binding cleft of the wild-type MAV proteinase wMAV PR. These were changed to the residues occupying a similar or identical position in the HIV-1 enzyme. The resultant mutated MAV proteinase (mMAV PR) exhibits increased enzymatic activity, altered substrate specificity, a substantially changed pH activity profile and a higher pH stability close to that observed in the HIV-1 PR. This dramatic alteration of MAV PR activity achieved by site-directed mutagenesis suggests that we have identified the amino acid residues contributing substantially to the differences between MAV and HIV-1 proteinases
Low-Temperature Carbon Capture Using Aqueous Ammonia and Organic Solvents
Current postcombustion CO<sub>2</sub> capture technologies are
energy intensive, require high-temperature heat sources, and dramatically
increase the cost of power generation. In this work, we introduce
a new carbon capture process requiring significantly lower temperatures
and less energy, creating further impetus to reduce CO<sub>2</sub> emissions from power generation. In this process, high-purity CO<sub>2</sub> is generated through the addition of an organic solvent (acetone,
dimethoxymethane, or acetaldehyde) to a CO<sub>2</sub> rich, aqueous
ammonia/carbon dioxide solution under room-temperature and -pressure
conditions. The organic solvent and CO<sub>2</sub>-absorbing solution
are then regenerated using low-temperature heat. When acetone, dimethoxymethane,
or acetaldehyde was added at a concentration of 16.7% (v/v) to 2 M
aqueous ammonium bicarbonate, 39.8, 48.6, or 86.5%, respectively,
of the aqueous CO<sub>2</sub> species transformed into high-purity
CO<sub>2</sub> gas over 3 h. Thermal energy and temperature requirements
for recovering acetaldehyde, the best-performing organic solvent investigated,
and the CO<sub>2</sub>-absorbing solution were 1.39 MJ/kg of CO<sub>2</sub> generated and 68 °C, respectively, 75% less energy than
the amount used in a pilot chilled ammonia process and a temperature
53 °C lower. Our findings exhibit the promise of economically
viable carbon capture powered entirely by abundant low-temperature
waste heat