Single cell measurement of telomerase expression and splicing using microfluidic emulsion cultures.

Telomerase is a reverse transcriptase that maintains telomeres on the ends of chromosomes, allowing rapidly dividing cells to proliferate while avoiding senescence and apoptosis. Understanding telomerase gene expression and splicing at the single cell level could yield insights into the roles of telomerase during normal cell growth as well as cancer development. Here we use droplet-based single cell culture followed by single cell or colony transcript abundance analysis to investigate the relationship between cell growth and transcript abundance of the telomerase genes encoding the RNA component (hTR) and protein component (hTERT) as well as hTERT splicing. Jurkat and K562 cells were examined under normal cell culture conditions and during exposure to curcumin, a natural compound with anti-carcinogenic and telomerase activity-reducing properties. Individual cells predominantly express single hTERT splice variants, with the α+/β- variant exhibiting significant transcript abundance bimodality that is sustained through cell division. Sub-lethal curcumin exposure results in reduced bimodality of all hTERT splice variants and significant upregulation of alpha splicing, suggesting a possible role in cellular stress response. The single cell culture and transcript abundance analysis method presented here provides the tools necessary for multiparameter single cell analysis which will be critical for understanding phenotypes of heterogeneous cell populations, disease cell populations and their drug response

Analysis of Irrigated Corn Production Adoption Decisions in Alabama

Expanding ethanol markets, abundance of water resources, and predominance of rainfed corn production in Alabama suggest possibility of irrigating cornfields. Numerical analysis shows that irrigated production becomes more preferable with higher corn prices and risk aversion. Adoption threshold is estimated at the price below its current level.Agribusiness, Crop Production/Industries, Land Economics/Use,

Spatial gene frequency waves under genotype dependent dispersal

Dispersal is a crucial factor in natural evolution, since it determines the habitat experienced by any population and defines the spatial scale of interactions between individuals. There is compelling evidence for systematic differences in dispersal characteristics within the same population, i.e., genotype-dependent dispersal. The consequences of genotype-dependent dispersal on other evolutionary phenomena, however, are poorly understood. In this article we investigate the effect of genotype-dependent dispersal on spatial gene frequency patterns, using a generalization of the classical diffusion model of selection and dispersal. Dispersal is characterized by the variance of dispersal (diffusion coefficient) and the mean displacement (directional advection term). We demonstrate that genotype-dependent dispersal may change the qualitative behavior of Fisher waves, which change from being “pulled” to being “pushed” wave fronts as the discrepancy in dispersal between genotypes increases. The speed of any wave is partitioned into components due to selection, genotype-dependent variance of dispersal, and genotype-dependent mean displacement. We apply our findings to wave fronts maintained by selection against heterozygotes. Furthermore, we identify a benefit of increased variance of dispersal, quantify its effect on the speed of the wave, and discuss the implications for the evolution of dispersal strategies

Reorganizing through Lean Principles

In August 2014, the director of the Collections, Acquisitions, and Discovery division within the University of Nevada, Las Vegas (UNLV) Libraries announced that the division would be reorganized to best use the skills and strengths of existing staff. This division, responsible for acquiring, organizing, maintaining, and making discoverable all needed and relevant materials would be composed of three departments, and the Resource Acquisition, Sharing, and Digital Scholarship department (containing the Resource Acquisition and Sharing Unit) would be one of them. To fulfill the goals of the reorganization, the acquisitions team used Lean principles and tools, specifically the value stream map, to understand and improve its processes

The Importance of Being Lean: Using Lean Principles and Tools to Improve Acquisitions Workflows

This presentation demonstrated how the UNLV University Libraries Acquisitions team is using Lean principles to analyze and improve acquisitions processes for firm and approval print and electronic monographic workflows. Lean process improvement is a system of concepts and tools to help an organization provide high value and high quality to our users in an efficient manner. In this session, the presenters provided a brief overview of lean principles and how this system can be adapted to a library setting. The presenters showed working examples of Lean-specific tools, like a Value-Stream map, that helped improve the UNLV Libraries acquisitions process

Propagule Pressure and Introduction Pathways of \u3cem\u3eBromus Tectorum\u3c/em\u3e (Cheatgrass; Poaceae) in the Central United States

An introduced species\u27 propagule pressure strongly influences the genetic diversity and evolutionary potential of its descendants and even the likelihood of biological invasion. We examined population genetic consequences arising from introduction of the invasive annual grass Bromus tectorum into the central United States (U.S.). The origin and frequency of introductions were investigated by assembling allozyme diversity data from 60 widely spaced populations. At least five introduction events contributed to the grass’s genetic diversity in the central U.S. Populations in this region have fewer alleles (30 vs. 43) and polymorphic loci (5 vs. 13) than native populations, evidence of a genetic bottleneck. However, the populations are, on average, more genetically diverse and less structured than native populations. Assembly within central U.S. populations of previously allopatric genotypes may have allowed the formation, via outcrossing, of a rare multilocus genotype. Genetic admixtures may have occurred through any combination of east-to-west spread coincident with nineteenth-century arrival of European settlers, dispersal from southern Ontario via Great Lakes shipping and commerce, and direct introduction from the native range. Our results illustrate the population genetic consequences of relatively high propagule pressure (i.e., repeated immigrations to a new range from multiple sources)

Stability of a flexible insert in one wall of an inviscid channel flow

A hybrid of computational and theoretical methods is extended and used to investigate the instabilities of a flexible surface inserted into one wall of an otherwise rigid channel conveying an inviscid flow. The computational aspects of the modelling combine finite-difference and boundary-element methods for structural and fluid elements respectively. The resulting equations are coupled in state-space form to yield an eigenvalue problem for the fluid–structure system. In tandem, the governing equations are solved to yield an analytical solution applicable to inserts of infinite length as an approximation for modes of deformation that are very much shorter than the overall length of the insert. A comprehensive investigation of different types of inserts – elastic plate, damped flexible plate, tensioned membrane and spring-backed flexible plate – is conducted and the effect of the proximity of the upper channel wall on stability characteristics is quantified. Results show that the presence of the upper-channel wall does not significantly modify the solution morphology that characterises the corresponding open-flow configuration, i.e. in the absence of the rigid upper channel wall. However, decreasing the channel height is shown to have a very significant effect on instability-onset flow speeds and flutter frequencies, both of which are reduced. The channel height above which channel-confinement effects are negligible is shown to be of the order of the wavelength of the critical mode at instability onset. For spring-backed flexible plates the wavelength of the critical mode is much shorter than the insert length and we show very good agreement between the predictions of the analytical and the state-space solutions developed in this paper. The small discrepancies that do exist are shown to be caused by an amplitude modulation of the critical mode on an insert of finite length that is unaccounted for in the travelling-wave assumption of the analytical model. Overall, the key contribution of this paper is the quantification of the stability bounds of a fundamental fluid–structure interaction (FSI) system which has hitherto remained largely unexplored

Haplotype-aware graph indexes

The variation graph toolkit (VG) represents genetic variation as a graph. Each path in the graph is a potential haplotype, though most paths are unlikely recombinations of true haplotypes. We augment the VG model with haplotype information to identify which paths are more likely to be correct. For this purpose, we develop a scalable implementation of the graph extension of the positional Burrows-Wheeler transform. We demonstrate the scalability of the new implementation by indexing the 1000 Genomes Project haplotypes. We also develop an algorithm for simplifying variation graphs for k-mer indexing without losing any k-mers in the haplotypes

Selected Songs of Dan Beaty: Background, Analysis, and Performance Guide

Dan Beaty (1937-2002) was a prolific composer, pianist, researcher, educator, and writer. His large compositional output included chamber works, choral works, songs, orchestral pieces, electronic music, and keyboard works. Beaty was well versed in traditional Western music as well as the more avant-garde and perplexing idioms of the twentieth century. Beaty's compositions reflect the many fascinating, if not always popular, musical trends of his time. His music encompasses styles from serial to jazz, shows compositional influences from Arnold Schoenberg to Indonesian music, and demonstrates thought-provoking and highly intellectual craftsmanship. This document explores several of Beaty's songs through a discussion of the composer's life and compositional process. Songs included in this document are Three Weeks Songs, October, November, A Sappho Lyric, Love Song, That Night When Joy Began, and War Lyrics. This document was written to accompany the author's DMA Lecture-Recital at the University of North Texas. Unfortunately, Beaty's vocal music was never published and is mostly unknown. One goal of the project was to initiate interest in Beaty's songs. Through this document, Lecture-Recital, and additional performances, considerable strides have been made to bring Beaty's songs to new audiences throughout the United States. In addition, the author has received permission from the Beaty family to publish Dan Beaty's songs