The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

Triclosan is a widespread antimicrobial agent that accumulates in anaerobic digesters used to treat the residual solids generated at municipal wastewater treatment plants; there is very little information, however, about how triclosan impacts microbial communities in anaerobic digesters. We investigated how triclosan impacts the community structure, function and antimicrobial resistance genes in lab-scale anaerobic digesters. Previously exposed (to triclosan) communities were amended with 5, 50, and 500 mg/kg of triclosan, corresponding to the median, 95th percentile, and 4-fold higher than maximum triclosan concentration that has been detected in U.S. biosolids. Triclosan amendment caused all of the Bacteria and Archaea communities to structurally diverge from that of the control cultures (based on ARISA). At the end of the experiment, all triclosan-amended Archaea communities had diverged from the control communities, regardless of the triclosan concentration added. In contrast, over time the Bacteria communities that were amended with lower concentrations of triclosan (5 mg/kg and 50 mg/kg) initially diverged and then reconverged with the control community structure. Methane production at 500 mg/kg was nearly half the methane production in control cultures. At 50 mg/kg, a large variability in methane production was observed, suggesting that 50 mg/kg may be a tipping point where function begins to fail in some communities. When previously unexposed communities were exposed to 500 mg triclosan/kg, function was maintained, but the abundance of a gene encoding for triclosan resistance (mexB) increased. This research suggests that triclosan could inhibit methane production in anaerobic digesters if concentrations were to increase and may also select for resistant Bacteria. In both cases, microbial community composition and exposure history alter the influence of triclosan

The Impacts of Triclosan on Anaerobic Community Structures, Function, and Antimicrobial Resistance

Triclosan is a widespread antimicrobial agent that accumulates in anaerobic digesters used to treat the residual solids generated at municipal wastewater treatment plants; there is very little information, however, about how triclosan impacts microbial communities in anaerobic digesters. We investigated how triclosan impacts the community structure, function and antimicrobial resistance genes in lab-scale anaerobic digesters. Previously exposed (to triclosan) communities were amended with 5, 50, and 500 mg/kg of triclosan, corresponding to the median, 95th percentile, and 4-fold higher than maximum triclosan concentration that has been detected in U.S. biosolids. Triclosan amendment caused all of the Bacteria and Archaea communities to structurally diverge from that of the control cultures (based on ARISA). At the end of the experiment, all triclosan-amended Archaea communities had diverged from the control communities, regardless of the triclosan concentration added. In contrast, over time the Bacteria communities that were amended with lower concentrations of triclosan (5 mg/kg and 50 mg/kg) initially diverged and then reconverged with the control community structure. Methane production at 500 mg/kg was nearly half the methane production in control cultures. At 50 mg/kg, a large variability in methane production was observed, suggesting that 50 mg/kg may be a tipping point where function begins to fail in some communities. When previously unexposed communities were exposed to 500 mg triclosan/kg, function was maintained, but the abundance of a gene encoding for triclosan resistance (mexB) increased. This research suggests that triclosan could inhibit methane production in anaerobic digesters if concentrations were to increase and may also select for resistant Bacteria. In both cases, microbial community composition and exposure history alter the influence of triclosan

Anisotropy of the Microwave Sky at 90 GHz: Results from Python II

We report on additional observations of degree scale anisotropy at 90~GHz from the Amundsen-Scott South Pole Station in Antarctica. Observations during the first season with the Python instrument yielded a statistically significant sky signal; in this paper we report the confirmation of that signal with data taken in the second year, and on results from an interleaving set of fields.Comment: 10 pages, plus 2 figures. Postscript and uufiles versions available via anonymous ftp at ftp://astro.uchicago.edu/pub/astro/ruhl/pyI

Radius Dependent Luminosity Evolution of Blue Galaxies in GOODS-N

We examine the radius-luminosity (R-L) relation for blue galaxies in the Team Keck Redshift Survey (TKRS) of GOODS-N. We compare with a volume-limited, Sloan Digital Sky Survey sample and find that the R-L relation has evolved to lower surface brightness since z=1. Based on the detection limits of GOODS this can not be explained by incompleteness in low surface-brightness galaxies. Number density arguments rule out a pure radius evolution. It can be explained by a radius dependent decline in B-band luminosity with time. Assuming a linear shift in M_B with z, we use a maximum likelihood method to quantify the evolution. Under these assumptions, large (R_{1/2} > 5 kpc), and intermediate sized (3 < R_{1/2} < 5 kpc) galaxies, have experienced Delta M_B =1.53 (-0.10,+0.13) and 1.65 (-0.18, +0.08) magnitudes of dimming since z=1. A simple exponential decline in star formation with an e-folding time of 3 Gyr can result in this amount of dimming. Meanwhile, small galaxies, or some subset thereof, have experienced more evolution, 2.55 (+/- 0.38) magnitudes. This factor of ten decline in luminosity can be explained by sub-samples of starbursting dwarf systems that fade rapidly, coupled with a decline in burst strength or frequency. Samples of bursting, luminous, blue, compact galaxies at intermediate redshifts have been identified by various previous studies. If there has been some growth in galaxy size with time, these measurements are upper limits on luminosity fading.Comment: 34 Total pages, 15 Written pages, 19 pages of Data Table, 13 Figures, accepted for publication in Ap

Time relaxation of interacting single--molecule magnets

We study the relaxation of interacting single--molecule magnets (SMMs) in both spatially ordered and disordered systems. The tunneling window is assumed to be, as in Fe8, much narrower than the dipolar field spread. We show that relaxation in disordered systems differs qualitatively from relaxation in fully occupied cubic and Fe_8 lattices. We also study how line shapes that develop in ''hole--digging'' experiments evolve with time t in these fully occupied lattices. We show (1) that the dipolar field h scales as t^p in these hole line shapes and show (2) how p varies with lattice structure. Line shapes are not, in general, Lorentzian. More specifically, in the lower portion of the hole, they behave as (h/t^p)^{(1/p)-1} if h is outside the tunnel window. This is in agreement with experiment and with our own Monte Carlo results.Comment: 21 LaTeX pages, 6 eps figures. Submitted to PRB on 15 June 2005. Accepted on 13 August 200

Galaxy size trends as a consequence of cosmology

We show that recently documented trends in galaxy sizes with mass and redshift can be understood in terms of the influence of underlying cosmic evolution; a holistic view which is complimentary to interpretations involving the accumulation of discreet evolutionary processes acting on individual objects. Using standard cosmology theory, supported with results from the Millennium simulations, we derive expected size trends for collapsed cosmic structures, emphasising the important distinction between these trends and the assembly paths of individual regions. We then argue that the observed variation in the stellar mass content of these structures can be understood to first order in terms of natural limitations of cooling and feedback. But whilst these relative masses vary by orders of magnitude, galaxy and host radii have been found to correlate linearly. We explain how these two aspects will lead to galaxy sizes that closely follow observed trends and their evolution, comparing directly with the COSMOS and SDSS surveys. Thus we conclude that the observed minimum radius for galaxies, the evolving trend in size as a function of mass for intermediate systems, and the observed increase in the sizes of massive galaxies, may all be considered an emergent consequence of the cosmic expansion.Comment: 14 pages, 13 figures. Accepted by MNRA