Berberine-Induced Amelioration of the Pathological Changes in Nutrient's Homeostasis During Murine Intestinal Eimeria papillata Infection

Abstract.-The current work aimed to study the ameliorative effect of berberine on the induced pathological changes in nutrient's homeostasis in mice infected with Eimeria papillata. Mice were randomly divided into three groups. The first group represents the control non-infected animals. Second and third groups were orally infected with 1.5×10 3 sporulated E. papillata oocysts. The 3 rd group was treated with a daily dose (10 mg/kg) of berberine chloride solution for five successive days. All animals were sacrificed on day 5 p.i.. E. papillata infection induced a state of disturbance in nutrient homeostasis. Blood glucose levels and total proteins were elevated with concurrent decrease in level of carbohydrates and soluble proteins in jejunum of mice. Also, infection induced a hyperlipidemic status as shown from the increase in triglycerides, total lipids, total cholesterol, high density lipoprotein cholesterol (LDL) with the mutual decrease in high density lipoprotein cholesterol (HDL) and phospholipids. Also, E. papillata caused marked disturbance in blood metal ion concentrations. Both ferrous and selenium ion levels were decreased, while sodium and potassium ion concentrations were increased. Berberine treatment of infected mice with E. papillata showed a great enhancement in nutrient homeostatic status and also reduced blood glucose level and restored jejunal carbohydrate content. In addition, berberine exerted hypolipidemic effect on the increased fractions of carbohydrates and lipids. Finally, berberine showed a marked enhancement in the levels of altered blood metal ions by the infection. Palm pollen grains or their extracts could be used within food mixtures or water to correct the induced metabolic disturbance and growth depression associated with the intestinal coccidial infections

Variation in the helminth community structure in spiny mice (Acomys dimidiatus) from four montane wadis in the St Katherine region of the Sinai Peninsula in Egypt

We compared helminth communities in spiny mice (Acomys dimidiatus) from 4 wadis in the arid montane region of the southern Sinai in Egypt, in a 4-week period in late summer. Total helminth species richness was 14 (8 nematodes, 5 cestodes and 1 acanthocephalan) with 94% of mice carrying at least 1 species and an overall mean species richness of 1.85. The most prevalent parasites were Protospirura muricola (47.8%) and Dentostomella kuntzi (46.3%). One larval cestode, Joyeuxiella rossicum, represents a new host record. The helminth community was dominated by intestinal nematodes (88.7%) of which 58.2% were arthropod-transmitted heteroxenic species. At the component community level, 70% of the worms were recovered from mice in just two wadis (Gharaba and Tlah) and 48.6% of intestinal nematodes were from Wadi Gharaba. Although only 7 species of helminths were recorded from Wadi Gharaba, this site gave the highest Berger-Parker dominance index because P. muricola. P. muricola was also dominant in Wadi El Arbaein whilst Syphacia minuta was the dominant species in Wadis Gebal and Tlah. At the infracommunity level, mean species richness and Brillouin’s index of diversity were highest in Wadi Tlah and lowest in Wadi Gebal, and the former was age dependent. Whilst mice from different wadis differed in the nematodes that were most common, those from Wadi Gharaba carried the highest mean number of worms/mouse. The abundance of P. muricola in particular varied markedly between sites: Wadi Gharaba was distinct as the site showing the highest mean worm burden whereas mice from Wadi Gebal were uninfected. None of the directly transmitted oxyuroid nematodes showed significant variation in abundance between wadis, or host sex or age classes. Overall, the single extrinsic factor in the study, site of capture, was more important than the intrinsic factors in explaining variation in helminth communities in the region. We conclude that in the high mountains of southern Sinai, each wadi is distinct in terms of its rodent parasites, and hence we expect spatially different coevolutionary pressures on their hosts, with resultant variation in life-histories

Haematological profile and parasitological survey of the domestic goats and camels of St Katherine, Sinai, Egypt

Haematological estimations and faecal egg counts were made on 32 goats and 43 camel sampled from different wadis of St. Katherine Protectorate, Sinai, Egypt during August-September 2000. Erythrocyte counts, platelet counts, packed cell volume, haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and total leukocyte counts were estimated. The percentage of neutrophils, eosinophils, basophils, monocytes and lymphocytes were determined from differential counts of leukocytes. In both animals, platelet counts and mean corpuscular haemoglobin concentrations showed significant differences among wadis; in goats, such differences were also present in total leukocyte counts and % of eosinophils. In goats, lymphocytes were more abundant than neutrophils in the blood. The prevalence of parasitic infection showed that 15% of the camels were infested with gastrointestinal helminths whereas 24% of goats were infested. KEY WORDS: haematology, gastrointestinal helminths, goats, camels, Sinai, Egypt Egyptian Journal of Biology Vol.4 2002: 101-10

A technique for MRI-guided transrectal deep pelvic abscess drainage

The purpose of this article is to introduce a technique for transrectal drainage of deep pelvic abscesses performed under interactive MRI guidance. A new method for triorthogonal image plane MRI guidance was developed and used to interactively monitor the puncture needle on continuously updated sets of adjustable three-plane images. The merits and limitations of the technique are highlighted and the patient population that is likely to benefit from this approach is suggested

Myocardial infarction presenting as both left ventricular aneurysm and ventricular septal defect

Acute myocardial infarction can result in various mechanical complications, although they have become rare with the advent of reperfusion therapies. Among these complications, ventricular septal rupture (VSR) and left ventricular aneurysm (LVA) are infrequent but life-threatening conditions associated with high morbidity and mortality. We present a rare case of a 67-year-old male with acute myocardial infarction who developed concomitant apical LVA and ventricular septal rupture

Investigation on New Metaheuristic Algorithms for Solving Dynamic Combined Economic Environmental Dispatch Problems

In this paper, the dynamic combined economic environmental dispatch problems (DCEED) with variable real transmission losses are tackled using four metaheuristics techniques. Due to the consideration of the valve-point loading effects (VPE), DCEED have become a non-smooth and more complex optimization problem. The seagull optimization algorithm (SOA), crow search algorithm (CSA), tunicate swarm algorithm (TSA), and firefly algorithm (FFA), as both nature and biologic phenomena-based algorithms, are investigated to solve DCEED problems. Our proposed algorithms, SOA, TSA, and FFA, were evaluated and applied on the IEEE five-unit test system, and the effectiveness of the proposed CSA approach was applied on two-unit, five-unit, and ten-unit systems by considering VPE. We defined CSA for different objective functions, such as cost of production, emission, and CEED, by considering VPE. The obtained results reveal the efficiency and robustness of the CSA compared to SOA, TSA, FFA, and to other optimization algorithms reported recently in the literature. In addition, Matlab simulation results show the advantages of the proposed approaches for solving DCEED problems

Investigation on New Metaheuristic Algorithms for Solving Dynamic Combined Economic Environmental Dispatch Problems

In this paper, the dynamic combined economic environmental dispatch problems (DCEED) with variable real transmission losses are tackled using four metaheuristics techniques. Due to the consideration of the valve-point loading effects (VPE), DCEED have become a non-smooth and more complex optimization problem. The seagull optimization algorithm (SOA), crow search algorithm (CSA), tunicate swarm algorithm (TSA), and firefly algorithm (FFA), as both nature and biologic phenomena-based algorithms, are investigated to solve DCEED problems. Our proposed algorithms, SOA, TSA, and FFA, were evaluated and applied on the IEEE five-unit test system, and the effectiveness of the proposed CSA approach was applied on two-unit, five-unit, and ten-unit systems by considering VPE. We defined CSA for different objective functions, such as cost of production, emission, and CEED, by considering VPE. The obtained results reveal the efficiency and robustness of the CSA compared to SOA, TSA, FFA, and to other optimization algorithms reported recently in the literature. In addition, Matlab simulation results show the advantages of the proposed approaches for solving DCEED problems