Search for the Heliospheric Termination Shock (TS) and Heliosheath (HS)

Voyager 1 continues to measure the very distant Heliospheric Magnetic Field (HMF) beyond 95 AU at ~35 North latitude. The MAG instrument data covers more than a full 22 years solar magnetic cycle. The magnitude of the observed HMF is well described, on average, by Parker's Archimedean spiral structure if due account is made for time variations of the source field strength and solar wind velocity. The V1 magnetic field observations do not provide any evidence for a field increase associated with entry into a subsonic solar wind region, such as the heliosheath is expected to be, nor an exit from this regime. We see no evidence for crossing of the Termination Shock (TS) as has been reported at ~85 AU by the LECP instrument. Merged Interaction Regions are identified by an increased HMF and associated decreases in the flux of >70 MeV/nuc cosmic rays which are then followed by a flux recovery. This CR-B relationship has been identified in V1 data and studied since 1982 when V1 was at 11 AU. The variance of HMF, a direct measure of the energy**1/2 in the HMF fluctuations, shows no significant changes associated with the alleged TS crossings in 2002–2003. Thus, the absence of any HMF increase at the entry into the heliosheath appears not to be due to the onset of mesoscale turbulence as proposed by Fisk. The TS has yet to be directly observed in-situ by the V1 MAG experiment in data through 2003

Review of broad-scale drought monitoring of forests: Toward an integrated data mining approach

Efforts to monitor the broad-scale impacts of drought on forests often come up short. Drought is a direct stressor of forests as well as a driver of secondary disturbance agents, making a full accounting of drought impacts challenging. General impacts can be inferred from moisture deficits quantified using precipitation and temperature measurements. However, derived meteorological indices may not meaningfully capture drought impacts because drought responses can differ substantially among species, sites and regions. Meteorology-based approaches also require the characterization of current moisture conditions relative to some specified time and place, but defining baseline conditions over large, ecologically diverse regions can be as difficult as quantifying the moisture deficit itself. In contrast, remote sensing approaches attempt to observe immediate, secondary, and longer-term changes in vegetation response, yet they too are no panacea. Remote sensing methods integrate responses across entire mixed-vegetation pixels and rarely distinguish the effects of drought on a single species, nor can they disentangle drought effects from those caused by various other disturbance agents. Establishment of suitable baselines from remote sensing may be even more challenging than with meteorological data. Here we review broad-scale drought monitoring methods, and suggest that an integrated data-mining approach may hold the most promise for enhancing our ability to resolve drought impacts on forests. A big-data approach that integrates meteorological and remotely sensed data streams, together with other data sets such as vegetation type, wildfire occurrence and pest activity, can clarify direct drought effects while filtering indirect drought effects and consequences. This strategy leverages the strengths of meteorology-based and remote sensing approaches with the aid of ancillary data, such that they complement each other and lead toward a better understanding of drought impacts

A robust method for the amplification of RNA in the sense orientation

BACKGROUND: Small quantities of RNA (1–4 μg total RNA) available from biological samples frequently require a single round of amplification prior to analysis, but current amplification strategies have limitations that may restrict their usefulness in downstream genomic applications. The Eberwine amplification method has been extensively validated but is limited by its ability to produce only antisense RNA. Alternatives lack extensive validation and are often confounded by problems with bias or yield attributable to their greater biological and technical complexity. RESULTS: To overcome these limitations, we have developed a straightforward and robust protocol for amplification of RNA in the sense orientation. This protocol is based upon Eberwine's method but incorporates elements of more recent amplification techniques while avoiding their complexities. Our technique yields greater than 100-fold amplification, generates long transcript, and produces mRNA that is well suited for use with microarray applications. Microarrays performed with RNA amplified using this protocol demonstrate minimal amplification bias and high reproducibility. CONCLUSION: The protocol we describe here is readily adaptable for the production of sense or antisense, labeled or unlabeled RNA from intact or partially-degraded prokaryotic or eukaryotic total RNA. The method outperforms several commercial RNA amplification kits and can be used in conjunction with a variety of microarray platforms, such as cDNA arrays, oligonucleotide arrays, and Affymetrix GeneChip™ arrays

Regression of tricuspid regurgitation after two-stage arterial switch operation for failing systemic ventricle after atrial inversion operation

AbstractThe cases of five patients with previous Senning ( n = 4) or Mustard ( n = 1) operations and failing systemic ventricles in whom banding of the pulmonary artery was performed as an interim step toward an arterial switch procedure are reported. The rise in the ratio of left to right mean systolic ventricular pressure, from 0.35 before operation to 0.90 during operation and 0.80 on the first postoperative day, caused a shift of the ventricular septum from a leftward to a midline or nearly midline position. This shift was associated with a reduction of tricuspid regurgitation. At a median interval of 5.1 months after pulmonary artery banding, the mean left ventricular posterior wall thickness had increased to 8.2 mm, versus 5 mm before operation, and the mean left ventricular myocardial mass index had increased to 90 gm/m2, versus 55.6 gm/m2 before operation. After the arterial switch operation, which was performed in four patients, the tricuspid regurgitation decreased to a trivial amount ( n = 1) or disappeared completely ( n = 3). (J THORAC CARDIOVASC SURG 1996;111:342-7

Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes

BACKGROUND: The autism spectrum encompasses a set of complex multigenic developmental disorders that severely impact the development of language, non-verbal communication, and social skills, and are associated with odd, stereotyped, repetitive behavior and restricted interests. To date, diagnosis of these neurologically based disorders relies predominantly upon behavioral observations often prompted by delayed speech or aberrant behavior, and there are no known genes that can serve as definitive biomarkers for the disorders. RESULTS: Here we demonstrate, for the first time, that lymphoblastoid cell lines from monozygotic twins discordant with respect to severity of autism and/or language impairment exhibit differential gene expression patterns on DNA microarrays. Furthermore, we show that genes important to the development, structure, and/or function of the nervous system are among the most differentially expressed genes, and that many of these genes map closely in silico to chromosomal regions containing previously reported autism candidate genes or quantitative trait loci. CONCLUSION: Our results provide evidence that novel candidate genes for autism may be differentially expressed in lymphoid cell lines from individuals with autism spectrum disorders. This finding further suggests the possibility of developing a molecular screen for autism based on expressed biomarkers in peripheral blood lymphocytes, an easily accessible tissue. In addition, gene networks are identified that may play a role in the pathophysiology of autism

Accessory and anomalous atrioventricular valvar tissue causing outflow tract obstruction Surgical implications of a heterogeneous and complex problem

AbstractObjectives. The purpose of this study was to determine the effect of accessory or anomalous atrioventricular valvar apparatus on relief of outflow tract obstruction.Background. Outflow tract obstruction due to accessory tissue or anomalous attachments of the atrioventricular valvar apparatus is an unusual but well-recognized problem. In addition to obstruction, anomalous attachments of the atrioventricular valvar apparatus may interfere with procedures to relieve outflow tract obstruction or perform outflow tract reconstruction.Methods. Since 1992, we have operated on 21 patients (median age 4 years) with systemic (n = 13), pulmonary (n = 5) or bilateral (n = 3) outflow tract obstruction due to accessory atrioventricular valvar tissue and/or anomalous attachments of the subvalvar apparatus. Primary diagnoses were isolated obstruction of the systemic outflow tract or aortic arch (n = 7), transposition complexes (n = 6), previously repaired atrioventricular septal defect (n = 3), functionally single ventricle (n = 3) and ventricular septal defect with pulmonary outflow obstruction (n = 2). Outflow tract gradients ranged from 20–110 mm Hg (median 58 mm Hg).Results. Complete relief of obstruction due to atrioventricular valvar anomalies was possible in 14 patients. In six patients, the planned procedure either had to be modified or only partial relief of the obstruction was achieved. In the remaining patient, who had borderline functionally single ventricle heart disease (unbalanced atrioventricular septal defect) and systemic outflow obstruction due to accessory and functional valvar apparatus, support was withdrawn because the parents refused univentricular palliation and the valvar anomalies precluded a Ross-Konno procedure. There were two early deaths. At follow-up ranging from 1 to 66 months (median 27 months), there was one death, and there has been no recurrence of outflow tract obstruction or residual atrioventricular valvar tissue.Conclusions. Outflow tract obstruction caused by accessory or anomalous atrioventricular valvar structures is an uncommon and heterogeneous group of conditions that can have significant surgical implications. In the majority of cases, tailoring of surgical techniques will permit complete relief of obstruction. However, such anomalies may limit standard surgical options and necessitate an innovative approach in some patients

Analisa Aliran Fluida Pada Pipa Spiral Dengan Variasi Diameter Menggunakan Metode Computational Fluid Dinamics (CFD)

Pipa merupakan alat transportasi fluida yang sangat murah, pipa memiliki berbagai ukuran dan bentuk penampang. Penurunan tekanan aliran didalam pipa sangat penting untuk diketahui guna merancang sistem perpipaan. Kekasaran pipa, panjang pipa, diameter pipa, jenis fluida, kecepatan dan bentuk aliran adalah hal yang sangat terkait dengan penurunan tekanan (Pressure Drop). Tujuan penelitian ini adalah untuk melihat efek dari perubahan diameter terhadap penurunan tekanan (pressure drop) pada pipa spiral. Simulasi dalam penelitian ini dilakukan untuk mengetahui secara teknis faktor penting pada penurunan tekanan (pressure drop) pada pipa spiral. Dengan bantuan aplikasi CFD dilakukan variasi diameter yang mempengaruhi penurunan tekanan

The Underestimation Of Egocentric Distance: Evidence From Frontal Matching Tasks

There is controversy over the existence, nature, and cause of error in egocentric distance judgments. One proposal is that the systematic biases often found in explicit judgments of egocentric distance along the ground may be related to recently observed biases in the perceived declination of gaze (Durgin & Li, Attention, Perception, & Psychophysics, in press), To measure perceived egocentric distance nonverbally, observers in a field were asked to position themselves so that their distance from one of two experimenters was equal to the frontal distance between the experimenters. Observers placed themselves too far away, consistent with egocentric distance underestimation. A similar experiment was conducted with vertical frontal extents. Both experiments were replicated in panoramic virtual reality. Perceived egocentric distance was quantitatively consistent with angular bias in perceived gaze declination (1.5 gain). Finally, an exocentric distance-matching task was contrasted with a variant of the egocentric matching task. The egocentric matching data approximate a constant compression of perceived egocentric distance with a power function exponent of nearly 1; exocentric matches had an exponent of about 0.67. The divergent pattern between egocentric and exocentric matches suggests that they depend on different visual cues

The Otterbein Miscellany - May 1965

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