Modeling and Full-Scale Testing of an Aspirating Face Seal

A 36" diameter aspirating face seal for aircraft engine application has undergone extensive testing and analysis. Previous testing indicated that the seal tended to seek equilibrium at axial rotor clearances that were larger than expected. Parameter studies were conducted on several seal design parameters to evaluate effect on seal performance. Mixing of air flows from the air dam and air bearing regions of the seal was shown to have a significant impact on the seal's performance. Two methods of minimizing this flow interaction were studied both analytically and experimentally. The first method is to reduce the labyrinth tooth clearance, thereby limiting flow to the air dam itself. The second method involves utilizing a flow deflector between the air dam and air bearing regions of the seal in order to prevent radial flow from the air dam from disrupting the formation of a hydrostatic film at the air bearing. Both methods were shown to be effective design enhancements, allowing seal closure to be achieved. In both cases, the seal seeks an equilibrium position 0.0015" from the rotor surface, with corresponding low leakage rates

Improved Steam Turbine Leakage Control With A Brush Seal Design.

LecturePg. 33-38Brush seal designs offer improved leakage control over conventional labyrinth seals by applying a compliant bristle with very tight clearance over the rotating shaft. Originally developed for the aircraft engine industry, brush seals have also been applied in land-based gas turbines. More recently, brush seals have been applied in steam turbines. The steam turbine environment adds some unique design considerations that must be addressed to assure a robust and effective brush seal design, and to minimize the impact on the steam turbine as a system. This paper discusses the performance benefits of the brush seal and the design considerations important to a robust design in a steam turbine. The paper also addresses the system characteristics important to reliable operation, and discusses the current experience basis from one original equipment manufacturer

Cloning, Expression, and Characterization of a Neuraminidase Gene from Arcanobacterium pyogenes

Arcanobacterium pyogenes is an opportunistic pathogen, associated with suppurative infections in domestic animals. In addition to pyolysin, a pore-forming, cholesterol-binding toxin, A. pyogenes expresses a number of putative virulence factors, including several proteases and neuraminidase activity. A 3,009-bp gene, nanH, was cloned and sequenced and conferred neuraminidase activity on an Escherichia coli host strain. The predicted 107-kDa NanH protein displayed similarity to a number of bacterial neuraminidases and contained the RIP/RLP motif and five copies of the Asp box motif found in all bacterial neuraminidases. Recombinant His-tagged NanH was found to have pH and temperature optima of 5.5 to 6.0 and 55°C, respectively. Insertional deletion of the nanH gene resulted in the reduction, but not absence, of neuraminidase activity, indicating the presence of a second neuraminidase gene in A. pyogenes. NanH was localized to the A. pyogenes cell wall. A. pyogenes adhered to HeLa, CHO, and MDBK cells in a washing-resistant manner. However, the nanH mutant was not defective for adherence to epithelial cells. The role of NanH in host epithelial cell adherence may be masked by the presence of a second neuraminidase in A. pyogenes