RFLP Markers and their relationship with a QTL in sheep

Restriction fragment length polymorphisms (RFLP's) were observed in EcoRI-, HindJIIand TaqI- digested Thai Longtail sheep genomic DNA probed with ovine FSH eDNA. RFLP was also detected in EcoRI-digested DNA ofthe same sheep breed with ovme thymus genomic DNA. No polymorphism was found in follistatin and ovine growth hormone (OGH) loci of the same sheep breed. When an analysis of variance between genotypes afthe simulated backcross data on body weight at 9 months was done, effect ofFSH genotypes s,howed significantly different effects on body weight but no such differing effect ofgenotypes of the thymus locus on body weight was observed

Functional properties of composite flour: A review

Incorporation of composite flour into wheat flour for bakery goods production is expected to produce an effect in the functional properties of the blended samples. Functional properties of composite flour have been studied in most of the developing countries which used and imported a large amount of wheat flour to fulfil the increasing number of consumers as the higher demand in the development of bakery and pastry products. In this review paper, the characteristics of composite flours were reviewed to determine the suitability of the raw materials to be used in the production of food products. The functional properties such as water and oil absorption capability, foam ability, emulsion capability, least gelation concentration, and particle size distribution might indicate the capability of the composite flour before proceeding to the development of food products were reviewed. The functionality of composite flour was found to be beneficial to enhance the variety of food products with acceptable appearance, organoleptic, nutrition, and low cost to fulfil consumer demands

Kandungan visual pembelajaran analitik data bagi menyokong pembelajaran bermakna

Tenaga pengajar bagi kursus analitik data berhadapan dengan cabaran mempersiap pelajar dengan kepelbagaian penggunaan data yang memerlukan pemahaman konsep dan perkaitan antara konsep yang lebih mendalam dalam kurikulum. Masalah pembelajaran analitik data membabitkan pelajar yang jarang mempunyai peluang untuk mengembangkan pemahaman secara mendalam tentang apa yang mereka pelajari dan pelajar cepat lupa setelah mereka menamatkan kursus. Pendekatan pembelajaran bermakna tidak tercapai selagi mana pelajar tidak menunjukkan komitmen bagi mengaitkan pengetahuan baharu kepada pembelajaran terdahulu. Pembelajaran bermakna tidak terlaksana dengan jayanya dalam pembelajaran analitik data tanpa melibatkan teknik pembelajaran bertepatan seperti visualisasi. Visualisasi data mampu mempersembah konsep dengan berkesan melalui ilustrasi dan grafik yang berasaskan pembelajaran sebenar dan autentik. Objektif kertas ini adalah untuk memperincikan kandungan visual dalam modul pembelajaran Analitik Data Visual (ADaVis) bagi pembelajaran analitik data pelajar institusi pengajian tinggi dan menguji keboleh gunaanmodul. Kajian ini adalah berasaskan kepada kajian reka bentuk sistem instruksional dan model reka bentuk sistem instruksional ADDIE. Modul pembelajaran ADaVis bertujuan membantu pelajar meningkatkan pemahaman konseptual analitik data dengan menyimpan konsep dipelajari dalam struktur kognitif yang kemas

Physicochemical, functional properties, in vitro starch digestibility and estimated glycaemic index of composite flour influenced by resistant starch

There has been increasing demand for resistant starch-enriched food products with a low glycaemic index (GI) as consumers nowadays are aware to improve health status. Therefore, the present study was carried out to investigate the effect of different substitution levels of type-2 resistant starch (high-amylose maize starch) (HM) into wheat flour. In this study, wheat flour sample (control) and six test composite flour samples comprising wheat flour substituted with 5%, 10%, 15%, 20%, 25% and 30% of HM powder were analyzed to compare their physicochemical characteristics, functional properties, in vitro starch digestibility and expected glycaemic index (eGI). The results revealed that the incorporation of HM had resulted in increased moisture (12.70 – 13.31%) and total dietary fibre (TDF) (0.19 – 0.46%), as well as a decreased proportion of ash, fat, and protein. The carbohydrate and energy values were not significantly different upon the increasing percentage of HM (p>0.05). Mineral analysis showed that HM composite flour had significantly lower Mg, Ca, K, P, Fe, Zn and Se than the control. HM composite flour exhibited greater water holding, water holding capacity, oil holding capacity and swelling power than the control sample. The hydrolysis index and eGI of HM composite flour decreased with higher HM substitution. In conclusion, HM composite flours showed a good potential to be used in functional food, where positive impacts have been observed for in vitro starch digestibility and eGI characteristic

Another look at follicular lymphoma: immunophenotypic and molecular analyses identify distinct follicular lymphoma subgroups

Aims The aim of this study was to analyse the immunophenotypic and molecular features of a large series of follicular lymphomas, focusing in particular on atypical cases that fail to express CD10 and/or bcl-2. Such cases present diagnostic pitfalls, especially with regard to the differential diagnosis from follicular hyperplasia and marginal zone B-cell lymphoma. Therefore, we also included an immunohistochemical evaluation of stathmin, which is strongly expressed by germinal centre B cells, as a putative new marker for follicular lymphomas, particularly those with an atypical phenotype. Methods and results Two hundred and five follicular lymphomas were investigated with immunohistochemistry and fluorescence in-situ hybridization (FISH). The use of three distinct anti-bcl-2 antibodies together with CD10 expression data and FISH analysis for bcl-2 and bcl-6 rearrangements allowed subclassification of follicular lymphoma into four distinct subgroups: (i) CD10-positive/bcl-2-positive, (ii) CD10-positive/bcl-2-negative, (iii) CD10-negative/bcl-2-positive, and (iv) CD10-negative/bcl-2-negative. All cases were bcl-6-positive. STMN1 (stathmin) was shown to be helpful in diagnosing bcl-2-negative and/or CD10-negative follicular lymphomas, and in their distinction from marginal zone B-cell lymphoma. Conclusions Combined immunohistological and molecular analyses reveal that follicular lymphomas showing an atypical immunophenotypic and molecular profile exist, and we demonstrate that STMN1 represents a novel useful diagnostic marker for these