Multiplexed two-photon excitation spectroscopy of single gold nanorods

Biological and Soft Matter Physic

Exploring molecular biology in sequence space: the road to next-generation single-molecule biophysics

Next-generation sequencing techniques have led to a new quantitative dimension in the biological sciences. In particular, integrating sequencing techniques with biophysical tools allows sequence-dependent mechanistic studies. Using the millions of DNA clusters that are generated during sequencing to perform high-throughput binding affinity and kinetics measurements enabled the construction of energy landscapes in sequence space, uncovering relationships between sequence, structure, and function. Here, we review the approaches to perform ensemble fluorescence experiments on next-generation sequencing chips for variations of DNA, RNA, and protein sequences. As the next step, we anticipate that these fluorescence experiments will be pushed to the single-molecule level, which can directly uncover kinetics and molecular heterogeneity in an unprecedented high-throughput fashion. Molecular biophysics in sequence space, both at the ensemble and single-molecule level, leads to new mechanistic insights. The wide spectrum of applications in biology and medicine ranges from the fundamental understanding of evolutionary pathways to the development of new therapeutics.Biological and Soft Matter Physic

Accuracy of the detection of binding events using 3d single particle tracking

Biological and Soft Matter Physic

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic

Multiplexing genetic and nucleosome positioning codes: A computational approach

Theoretical PhysicsBiological and Soft Matter Physic

Multiple aspects of atp-dependent nucleosome translocation by rsc and mi-2 are directed by the underlying dna sequence

Biological and Soft Matter Physic

Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding

Biological and Soft Matter Physic

Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate

Biological and Soft Matter PhysicsAnimal science

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic