Growth Profile and fatty acid accumulation of four Chaetoceros Taxa isolated from coastal water of Pahang, Malaysia

This indoor study was aimed to analyze the production of fatty acids with their growth profile from few marine algae under the genus Chaetoceros isolated from coastal water of Pahang, Malaysia. The algae were established into culture using standard marine media (f/2 media) and the variation of fatty acid for each species was determined using GCMS. Statistical analysis of one-way ANOVA was performed to evaluate the significant and homogeneity data on the growth of each alga and total fatty acid percentage obtained. The results showed that four taxa were successfully cultivated and identified as Chaetoceros baculites, Chaetoceros anastomosans, Chaetoceros affinis var. willei and Chaetoceros affinis var. affinis. Out of four Chaetoceros, C. baculites showed the highest growth rate (0.75 cell.day-1) and division’s value (1.08) while C. anastomosans showed the highest doubling time value (8.66). Statistical analysis showed that all species have significantly different growth rate (p<0.05). Myristic acid was the main component for fatty acid storage for C. baculites, C. anastomosans and C. affinis var. willei whereas palmitic acid for C. affinis var. affinis. All species contained about 35 to 75% of total percentage fatty acids throughout the growth day. Based on total percentage, both affinis varieties had high fatty acid percentage compared with the other two species with the total percentage of more than 70%. As a conclusion, all four taxa are suitable to be used in lipid industry in Malaysia with C. affinis var. affinis is the best candidate for bio-fuel industry and C. anastomosans for pharmaceutical industry

PCR assembly of synthetic human erythropoietin gene

Human erythropoietin (huEPO) is a glycoprotein with important physiological functions, such as erythropoiesis, angiogenesis, and wound healing. A therapeutic protein, huEPO is commonly used to treat patients suffering from renal and non-renal anemia. Recombinant human erythropoietin (rhuEPO) and endogenous huEPO are similar with respect to their biological and chemical properties. In this study, we describe the construction of synthetic huEPO gene to produce rhuEPO. The synthetic huEPO gene was constructed by overlapping oligonucleotides assembly and amplified by polymerase chain reaction (PCR). Twenty oligonucleotide sets, covering the huEPO gene sequence and two newly introduced restriction enzyme sites, were pulled together and amplified using Pfu DNA polymerase to produce the expected DNA products with sizes of ~500bp and ~600bp. The PCR products were ligated into pGEM-T plasmid vector to facilitate DNA sequencing process of the constructed huEPO gene and downstream cloning manipulation. DNA sequence analysis showed correctly assembled oligonucleotide sets, representing the huEPO gene sequence albeit with minor base mutations. Hence, oligonucleotides assembly and PCR amplification provide a convenient and speedy method for the synthesis of huEPO gene without depending on mRNA isolation and reverse transcription or the need to have a genomic library

The Effects Of Dry And Chilled Air On Tool Wear Behavior During Face Milling Of Inconel 718

Tool wear is one of the important criteria during the cutting process. It is mostly caused by the machining parameters, namely; cutting speed, feed rate, depth of cut, cooling condition, etc. This paper presents the behavior of cutting tool during dry and chilled air condition of face mill with the cutting speed of 20 to 40 m/min, the feed rate of 0.1 to 0.2 mm/tooth and axial depth of 0.1 mm. The analysis of variance (ANOVA) is applied to identify the significance of these factors effect on tool performance, later the mathematical model for the tool life prediction was developed. The investigation revealed that the cutting speed, feed rate dominating wear rate whilst the chilled air found to be marginally significant. Finally, the optimum condition for machining parameter for greater tool life can be obtained by the combination cutting speed of 20 m/min, the feed rate of 0.1 mm/tooth under chilled air condition. Implementation of chilled air contributed 7% improvement with 45 min compared to a dry condition. The study exhibited the round type insert of dry face milling is more prone to rapid flank wear than chilled air with no BUE appearance on the tool cutting edge

Pure cultivation and morphological studies of four chaetoceros taxa from the coastal waters of Pahang, Malaysia

This study was conducted to study the morphological features of species under the genus Chaetoceros isolated from the coastal waters of Pahang, Malaysia. The species were isolated, cultivated and viewed under light microscope (LM) and scanning electron microscope (SEM). Four taxa were successfully isolated and cultivated in pure culture, namely Chaetoceros affinis var. affinis, Chaetoceros affinis var. willei, Chaetoceros anastomosans and Chaetoceros baculites. Both varieties of C. affinis were considered as distinct taxa where both strains can be distinguished based on morphological characteristics. C. affinis var. affinis has a thick cell wall compared with C. affinis var. willei. C. anastomosans has special features including a silica bar on the intersection of setae and the production of mucous. The length of the aperture opening is a new record from this study. The new record obtained for C. baculites includes the size of the aperture, the terminal setae, spinal arrangement, the thinness of the cell wall and the location of the species in tropical waters. Detailed data on each species will be added to the taxonomic information for future studies

Single Stage String Inverter for Gridconnected Photovoltaic System with Modified Perturb and Observe (P&O) Fuzzy Logic Control(FLC)-based MPPT Technique

This paper presents an implementation of Single-phase Single stage String inverter for Grid connected Photovoltaic (PV) system. The proposed system uses Modified Perturb and Observe (P&O) algorithm implemented using Fuzzy Logic Control (FLC) as Maximum Power Point Tracking (MPPT). The inverter is designed for 340W system using two series of STP170s24/Ac PV modules. The MPPT unit keeps tracking the maximum power from the PV array by changing the modulation index and the phase angle of inverter’s output voltage. The simulation model is developed using Matlab/Simulink to evaluate the performance of the converter. Selected experimental results are also presented in this paper

Growth Profile and Fatty Acid Accumulation of Four Chaetoceros Species Isolated from Coastal Water of Pahang, Malaysia

This indoor study was aimed to analyze the production of fatty acids with their growth profile from few marine algae under the genus Chaetoceros isolated from coastal water of Pahang, Malaysia. The algae were established into culture using standard marine media (f/2 media) and the variation of fatty acid for each species was determined using GCMS. Statistical analysis of one-way ANOVA was performed to evaluate the significant and homogeneity data on the growth of each alga and total fatty acid percentage obtained. The results showed that four taxa were successfully cultivated and identified as Chaetoceros baculites, Chaetoceros anastomosans, Chaetoceros affinis var. willei and Chaetoceros affinis var. affinis. Out of four Chaetoceros, C. baculites showed the highest growth rate (0.75 cell.day-1) and division’s value (1.08) while C. anastomosans showed the highest doubling time value (8.66). Statistical analysis showed that all species have significantly different growth rate (p<0.05). Myristic acid was the main component for fatty acid storage for C. baculites, C. anastomosans and C. affinis var. willei whereas palmitic acid for C. affinis var. affinis. All species contained about 35 to 75% of total percentage fatty acids throughout the growth day. Based on total percentage, both affinis varieties had high fatty acid percentage compared with the other two species with the total percentage of more than 70%. As a conclusion, all four taxa are suitable to be used in lipid industry in Malaysia with C. affinis var. affinis is the best candidate for bio-fuel industry and C. anastomosans for pharmaceutical industry

The Malay great works students' perception towards interface design elements Massive Open Online Course (MOOC)

Technology learning, such as MOOC is necessary in modern society that ensures new knowledge and can be share. In teaching and learning, the use of MOOC will considered as a tool that can improve cognitive, psychomotor and affective to learners. MOOC, students can connect each other and free access that encourages borderless discussion. This study aims to identify issue and analyze student admissions to the MOOC design of BBK 4201 The Malay Great Works course. This study’s respondents involved 69 students of BBK 4201 The Malay Great Works at Universiti Putra Malaysia. This study’s emphasis will focus on the two main elements of design with the division of 8 items. Quantitative approaches will use to carry out this study. At the same time, the investigation will be using the Survey Monkey website and analyzed based on the Technology Acceptance Model (TAM) approach. This is because this approach will explicitly designed to analyze the technology acceptance. The study results show the level of tendency of students to be interested in MOOC design towards learning. The study results can also help give an overview to online course builders about face-to-face design that can increase student interest and understanding

PCR assembly of synthetic human erythropoietin gene

Human erythropoietin (huEPO) is a glycoprotein with important physiological functions, such as erythropoiesis, angiogenesis, and wound healing. A therapeutic protein, huEPO is commonly used to treat patients suffering from renal and non-renal anemia. Recombinant human erythropoietin (rhuEPO) and endogenous huEPO are similar with respect to their biological and chemical properties. In this study, we describe the construction of synthetic huEPO gene to produce rhuEPO. The synthetic huEPO gene was constructed by overlapping oligonucleotides assembly and amplified by polymerase chain reaction (PCR). Twenty oligonucleotide sets, covering the huEPO gene sequence and two newly introduced restriction enzyme sites, were pulled together and amplified using Pfu DNA polymerase to produce the expected DNA products with sizes of ~500bp and ~600bp. The PCR products were ligated into pGEM-T plasmid vector to facilitate DNA sequencing process of the constructed huEPO gene and downstream cloning manipulation. DNA sequence analysis showed correctly assembled oligonucleotide sets, representing the huEPO gene sequence albeit with minor base mutations. Hence, oligonucleotides assembly and PCR amplification provide a convenient and speedy method for the synthesis of huEPO gene without depending on mRNA isolation and reverse transcription or the need to have a genomic library