Modulating receptor-ligand binding in biorecognition by setting surface wettability

[EN] Modulation of support wettability used for microarray format biosensing has led to an improvement of results. Hydrophobicity of glass chips was set by derivatizing with single vinyl organosilanes of different chain length and silane mixtures. Thiol-ene photochemical linking has been used as effective chemistry for covalent anchoring of thiolated probes. Lowest unspecific binding and highest signal intensity and SNR were obtained with large hydrocarbon chain (C-22) silanes or a shorter one (C-10) containing fluorine atoms. SNR resulting values are improved, reaching levels higher than 1500 in some cases, when using vinyl silanes modified with 1% C-10 alkyl fluorinated one, because mild hydrophobicity was achieved (water contact angle ca. 110 degrees) for all silanes, including the short C-2 and C-3, thus giving rise to smaller and better defined array spots. In addition, unspecific binding of reagents and targets was totally withdrawn. Hence, good-performing surfaces for biosensing applications can be built using appropriate organosilane reagent selection, including fluorinated ones.We thank Patricia Concepcion and Dolores Soriano (Instituto de Tecnologia Quimica, Universitat Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas) for helping with XPS analysis. Financial support from the Generalitat Valenciana (GVA-PROMETEO/2014/040), FEDER, and the Spanish Ministry of Economy and Competitiveness (CTQ2013-45875-R and CTQ2016-75749-R) is acknowledged.Aragón Revuelta, P.; Noguera Murray, PS.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, A.; González Martínez, MÁ. (2018). Modulating receptor-ligand binding in biorecognition by setting surface wettability. Analytical and Bioanalytical Chemistry. 410(23):5723-5730. https://doi.org/10.1007/s00216-018-1247-8S5723573041023González-Martínez MA, Puchades R, Maquieira A. Optical immunosensors for environmental monitoring: how far have we come? Anal Bioanal Chem. 2007;387:205–18.Kontogeorgis GM, Kiil S. Intermolecular and interparticle forces. In: Kontogeorgis GM, Kiil S, editors. Introduction to applied colloid and surface chemistry. Chichester: Wiley; 2016. p. 11–33.Ronkainen NJ, Halsall HB, Heineman WR. Electrochemical immunoassays and immunosensors. In: van Emon JM, editor. Immunoassay and other bioanalytical methods. Boca Raton: CRC Press, Taylor & Francis Group; 2007. Chapter 16.González-Lucas D, Bañuls M-J, Puchades R, Maquieira A. Versatile and easy fabrication of advanced surfaces for high performance DNA microarrays. Adv Mater Interfaces. 2016:1500850.Yin L, Lu M, Wielunski L, Song W, Tan J, Lu Y, et al. Fabrication and characterization of compact silicon oxynitride waveguides on silicon chips. J Opt. 2012;14:085501.Zhang F, Sautter K, Larssen AM, Findley DA, Davis RC, Samha H, et al. Chemical vapor deposition of three aminosilanes on silicon dioxide: surface characterization, stability, effects of silane concentration, and cyanine dye adsorption. Langmuir. 2010;26:14648–54.Escorihuela J, Bañuls M-J, Puchades R, Maquieira A. DNA microarrays on silicon surfaces through thiol-ene chemistry. Chem Commun. 2012;48:2116–8.Kontogeorgis GM, Kiil S. Wetting and adhesion. In: Kontogeorgis GM, Kiil S, editors. Introduction to applied colloid and surface chemistry. Chichester: Wiley; 2016. p. 121–60.Weinrich D, Köhn M, Jonkheijm P, Westerlind U, Dehmelt L, Engelkamp H, et al. Preparation of biomolecule microstructures and microarrays by thiol-ene .photoimmobilization. ChemBioChem. 2010;11:235–47.Mira D, Llorente R, Morais S, Puchades R, Maquieira A, Marti J. High-throughput screening of surface-enhanced fluorescence on industrial standard digital recording media. In: Carrano JC, Zukauskas A, editors. Optically Based Biological and Chemical Sensing for Defence, Proceedings SPIE Vol. 5617. Bellingham: SPIE; 2004, 0277-786X/04/$15. https://doi.org/10.1117/12.578301 .Wasserman SR, Tao YT, Whitesides GM. Structure and reactivity of alkylsiloxane monolayers formed by reaction of alkyltrichlorosilanes on silicon substrates. Langmuir. 1989;5:1074–87.Munsch J-N, Dentzer J, Claus W, Bassel H. Structural characterization of silanes modified silica by temperature programmed desorption coupled to mass-spectra (TPD-MS) technique. J Colloid Sci Biotech. 2014;3:30–7.Celia E, Darmanin T, de Givenchy ET, Amigoni S, Guittard F. Recent advances in designing superhydrophobic surfaces. J Colloid Interface Sci. 2013;402:1–18.Li L, Li J, Du X, Welle A, Grunze M, Trapp O, et al. Direct UV-induced functionalization of surface hydroxy groups by thiol-ol chemistry. Angew Chem Int Ed. 2014;53:3835–9.Marczak J, Kargol M, Psarski M, Celichowski G. Modification of epoxy resin, silicon and glass surfaces with alkyl- orfluoroalkylsilanes for hydrophobic properties. Appl Surf Sci. 2016;380:91–100

Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples

[EN] Xanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips. The specificity of the LFIA was tested against 87 X. arboricola pv. pruni strains from different countries worldwide, 47 strains of other Xanthomonas species and 14 strains representing other bacterial genera. All X. arboricola pv. pruni strains were detected and cross-reactions were observed only with four strains of X. arboricola pv. corylina, a hazelnut pathogen that does not share habitat with X. arboricola pv. pruni. The sensitivity of the LFIA was assessed with suspensions from pure cultures of three X. arboricola pv. pruni strains and with spiked leaf extracts prepared from four hosts inoculated with this pathogen (almond, apricot, Japanese plum and peach). The limit of detection observed with both pure cultures and spiked samples was 10(4) CFU ml(-1). To demonstrate the accuracy of the test, 205 samples naturally infected with X. arboricola pv. pruni and 113 samples collected from healthy plants of several different Prunus species were analyzed with the LFIA. Results were compared with those obtained by plate isolation and real time PCR and a high correlation was found among techniques. Therefore, we propose this LFIA as a screening tool that allows a rapid and reliable diagnosis of X. arboricola pv. pruni in symptomatic plants.The work was supported by the following: Instituto Nacional de Tecnologia Agraria y Alimentaria, Project RTA-2011-00140-C03-01 (http://www.inia.es), PLS MTG EMN MML; Instituto Nacional de Tecnologia Agraria y Alimentaria, FPI-INIA grant (http://www.inia.es), PLS; Generalitat Valenciana (Prometeo II 2014/040) (http://www.gva.es), PN RP AM; Ministerio de Economia y Competitividad (MINECO) (CTQ2013-45875R) (http://www.mineco.gob.es), PN RP AM; European Social Fund, PLS MTG EMN MML; and European Regional Development Fund, PLS MTG EMN MML.López-Soriano, P.; Noguera Murray, PS.; Gorris, MT.; Puchades, R.; Maquieira Catala, A.; Marco-Noales, E.; López, M. (2017). Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples. PLoS ONE. 12(4):1-13. https://doi.org/10.1371/journal.pone.0176201113124Tjou-Tam-Sin, N. N. A., van de Bilt, J. L. J., Bergsma-Vlami, M., Koenraadt, H., Naktuinbouw, J. W., van Doorn, J., … Martin, W. S. (2012). First Report of Xanthomonas arboricola pv. pruni in Ornamental Prunus laurocerasus in the Netherlands. Plant Disease, 96(5), 759-759. doi:10.1094/pdis-04-11-0265-pdnPothier, J. F., Vorhölter, F.-J., Blom, J., Goesmann, A., Pühler, A., Smits, T. H. M., & Duffy, B. (2011). The ubiquitous plasmid pXap41 in the invasive phytopathogen Xanthomonas arboricola pv. pruni: complete sequence and comparative genomic analysis. FEMS Microbiology Letters, 323(1), 52-60. doi:10.1111/j.1574-6968.2011.02352.xPalacio-Bielsa, A., Cubero, J., Cambra, M. A., Collados, R., Berruete, I. M., & López, M. M. (2010). Development of an Efficient Real-Time Quantitative PCR Protocol for Detection ofXanthomonas arboricolapv. pruni inPrunusSpecies. Applied and Environmental Microbiology, 77(1), 89-97. doi:10.1128/aem.01593-10Xanthomonas arboricola pv. pruni. (2006). EPPO Bulletin, 36(1), 129-133. doi:10.1111/j.1365-2338.2006.00925.xPagani MC. An ABC transporter protein and molecular diagnoses of Xanthomonas arboricola pv. pruni causing bacterial spot of stone fruits. Raleigh, North Carolina, USA: North Carolina State University, PhD thesis. 2004; Online, http://repository.lib.ncsu.edu/ir/bitstream/1840.16/4540/1/etd.pdfPark, S. Y., Lee, Y. S., Koh, Y. J., Hur, J.-S., & Jung, J. S. (2010). Detection of Xanthomonas arboricola pv. pruni by PCR using primers based on DNA sequences related to the hrp genes. The Journal of Microbiology, 48(5), 554-558. doi:10.1007/s12275-010-0072-3Pothier, J. F., Pagani, M. C., Pelludat, C., Ritchie, D. F., & Duffy, B. (2011). A duplex-PCR method for species- and pathovar-level identification and detection of the quarantine plant pathogen Xanthomonas arboricola pv. pruni. Journal of Microbiological Methods, 86(1), 16-24. doi:10.1016/j.mimet.2011.03.019Ballard, E. L., Dietzgen, R. G., Sly, L. I., Gouk, C., Horlock, C., & Fegan, M. (2011). Development of a Bio-PCR Protocol for the Detection of Xanthomonas arboricola pv. pruni. Plant Disease, 95(9), 1109-1115. doi:10.1094/pdis-09-10-0650Boonham, N., Glover, R., Tomlinson, J., & Mumford, R. (2008). Exploiting generic platform technologies for the detection and identification of plant pathogens. European Journal of Plant Pathology, 121(3), 355-363. doi:10.1007/s10658-008-9284-3Posthuma-Trumpie, G. A., Korf, J., & van Amerongen, A. (2008). Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey. Analytical and Bioanalytical Chemistry, 393(2), 569-582. doi:10.1007/s00216-008-2287-2De Boer, S. H., & López, M. M. (2012). New Grower-Friendly Methods for Plant Pathogen Monitoring. Annual Review of Phytopathology, 50(1), 197-218. doi:10.1146/annurev-phyto-081211-172942Thornton, C. R., Groenhof, A. C., Forrest, R., & Lamotte, R. (2004). A One-Step, Immunochromatographic Lateral Flow Device Specific to Rhizoctonia solani and Certain Related Species, and Its Use to Detect and Quantify R. solani in Soil. Phytopathology®, 94(3), 280-288. doi:10.1094/phyto.2004.94.3.280Lane, C. R., Hobden, E., Walker, L., Barton, V. C., Inman, A. J., Hughes, K. J. D., … Barker, I. (2007). Evaluation of a rapid diagnostic field test kit for identification of Phytophthora species, including P. ramorum and P. kernoviae at the point of inspection. Plant Pathology, 56(5), 828-835. doi:10.1111/j.1365-3059.2007.01615.xSafenkova, I., Zherdev, A., & Dzantiev, B. (2012). Factors influencing the detection limit of the lateral-flow sandwich immunoassay: a case study with potato virus X. Analytical and Bioanalytical Chemistry, 403(6), 1595-1605. doi:10.1007/s00216-012-5985-8Safenkova, I. V., Pankratova, G. K., Zaitsev, I. A., Varitsev, Y. A., Vengerov, Y. Y., Zherdev, A. V., & Dzantiev, B. B. (2016). Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens. Analytical and Bioanalytical Chemistry, 408(22), 6009-6017. doi:10.1007/s00216-016-9463-6Braun-Kiewnick, A., Altenbach, D., Oberhänsli, T., Bitterlin, W., & Duffy, B. (2011). A rapid lateral-flow immunoassay for phytosanitary detection of Erwinia amylovora and on-site fire blight diagnosis. Journal of Microbiological Methods, 87(1), 1-9. doi:10.1016/j.mimet.2011.06.015Safenkova, I. V., Zaitsev, I. A., Pankratova, G. K., Varitsev, Y. A., Zherdev, A. V., & Dzantiev, B. B. (2014). Lateral flow immunoassay for rapid detection of potato ring rot caused by Clavibacter michiganensis subsp. sepedonicus. Applied Biochemistry and Microbiology, 50(6), 675-682. doi:10.1134/s0003683814120011Hodgetts, J., Karamura, G., Johnson, G., Hall, J., Perkins, K., Beed, F., … Smith, J. (2014). Development of a lateral flow device for in-field detection and evaluation of PCR-based diagnostic methods forXanthomonas campestrispv.musacearum, the causal agent of banana xanthomonas wilt. Plant Pathology, 64(3), 559-567. doi:10.1111/ppa.12289Noguera, P., Posthuma-Trumpie, G. A., van Tuil, M., van der Wal, F. J., de Boer, A., Moers, A. P. H. A., & van Amerongen, A. (2010). Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Analytical and Bioanalytical Chemistry, 399(2), 831-838. doi:10.1007/s00216-010-4334-zCambra M, López MM. Titration of Agrobacterium radiobacter var. tumefaciens antibodies by using enzyme labeled anti-rabbit γ-globulines (ELISA indirect method). In: Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, ed. Station Pathologie Végétale, INRA Angers, 1978. pp: 327–331.O’Keeffe, M., Crabbe, P., Salden, M., Wichers, J., Van Peteghem, C., Kohen, F., … Moneti, G. (2003). Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine. Journal of Immunological Methods, 278(1-2), 117-126. doi:10.1016/s0022-1759(03)00207-2PM 7/98 (2) Specific requirements for laboratories preparing accreditation for a plant pest diagnostic activity. (2014). EPPO Bulletin, 44(2), 117-147. doi:10.1111/epp.12118Lamichhane, J. R., & Varvaro, L. (2013). Xanthomonas arboricoladisease of hazelnut: current status and future perspectives for its management. Plant Pathology, 63(2), 243-254. doi:10.1111/ppa.12152Fischer-Le Saux, M., Bonneau, S., Essakhi, S., Manceau, C., & Jacques, M.-A. (2015). Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing. Applied and Environmental Microbiology, 81(14), 4651-4668. doi:10.1128/aem.00050-15Bühlmann, A., Pothier, J. F., Tomlinson, J. A., Frey, J. E., Boonham, N., Smits, T. H. M., & Duffy, B. (2012). Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenicXanthomonas arboricolapv. pruniat the intraspecific level. Plant Pathology, 62(2), 475-484. doi:10.1111/j.1365-3059.2012.02654.xGarita-Cambronero, J., Palacio-Bielsa, A., López, M. M., & Cubero, J. (2017). Pan-Genomic Analysis Permits Differentiation of Virulent and Non-virulent Strains of Xanthomonas arboricola That Cohabit Prunus spp. and Elucidate Bacterial Virulence Factors. Frontiers in Microbiology, 8. doi:10.3389/fmicb.2017.00573Ghezzi, J. I., & Steck, T. R. (1999). Induction of the viable but non-culturable condition in Xanthomonas campestris pv. campestris in liquid microcosms and sterile soil. FEMS Microbiology Ecology, 30(3), 203-208. doi:10.1111/j.1574-6941.1999.tb00648.xDel Campo, R., Russi, P., Mara, P., Mara, Hã©., Peyrou, M., de León, I. P., & Gaggero, C. (2009). Xanthomonas axonopodispv.citriâ enters the VBNC state after copper treatment and retains its virulence. FEMS Microbiology Letters, 298(2), 143-148. doi:10.1111/j.1574-6968.2009.01709.

Elaboración de materiales de apoyo para incorporar la competencia “Planificación y Gestión del Tiempo” en las asignaturas de la Universitat Politècnica de València

[EN] In recent years, Higher Education has undergone many changes with the aim of adapting University to the new social context, not only in terms of technical training -such as teaching methods and quality of the learning processes-, but also at student employability level -through the acquisition of transversal competencies, which have an increasing demand by companies-. Following these guidelines, the Universitat Politècnica de València (UPV) has included in its Strategic Plan UPV-2015-2020 the following objective: "to be a leader in quality teaching and training aimed to fulfill the needs of the Society”. One of the many initiatives undertaken to achieve this is the ambitious project where the development, evaluation and certification of thirteen transversal competencies are considered. In this context, this work shows the activities undertaken by the working group of the UPV dedicated to the transversal competency Time Planning and Management. Work undertaken includes: definition of three skill levels for the acquisition of this competency; development of rubrics and activities to work and evaluate this competency; and the production of audiovisual material. All of which are aimed to aid the incorporation of this competency in subjects of the UPV.[ES] Las reformas en Educación Superior llevadas a cabo en los últimos años van orientadas a adaptar la universidad a la nueva realidad social, tanto a nivel de formación técnica: metodologías docentes, estructura de las enseñanzas y calidad de los procesos de aprendizaje, como a nivel de empleabilidad del alumno a través de la adquisición de las competencias transversales cada vez más demandadas por las empresas. Siguiendo estas directrices, la Universitat Politècnica de València (UPV) ha establecido en su Plan Estratégico UPV 2015‐2020 el objetivo de “ser un referente en docencia y formación de calidad orientada a las necesidades de la Sociedad”. Para ello ha puesto en marcha múltiples iniciativas entre las que destaca la creación de un proyecto institucional de desarrollo, evaluación y acreditación de trece competencias transversales. En este contexto, la siguiente comunicación presenta las actividades desarrolladas por el grupo de trabajo de la UPV respecto a la competencia transversal Planificación y Gestión del Tiempo, que han abarcado desde la definición de los tres niveles de dominio de esta competencia, elaboración de rúbricas y diseño de actividades hasta la generación de material educativo de apoyo a la hora de incorporar dicha competencia en las asignaturas de la UPV.Cáceres González, PA.; Martínez García, A.; Noguera Murray, PS.; Pérez Marín, E.; Sanabria Codesal, E. (2016). Elaboración de materiales de apoyo para incorporar la competencia “Planificación y Gestión del Tiempo” en las asignaturas de la Universitat Politècnica de València. En In-Red 2016. II Congreso nacional de innovación educativa y docencia en red. Editorial Universitat Politècnica de València. https://doi.org/10.4995/INRED2016.2016.4346OC

Propiedades ácido-base de las sales

Este objeto de aprendizaje describe la metodología para calcular el pH de disoluciones ácidas o básicas (bien sean fuertes o débiles) y permitirá al alumno diferenciar entre ácidos fuertes y débiles, así como entre bases fuertes y débiles, calcular el pH de disoluciones de ácidos o bases fuertes y utilizar la constante de equilibrio de ácidos o bases débiles para el cálculo del pH.Morais Ezquerro, SB.; Noguera Murray, PS.; Tortajada Genaro, LA.; Atienza Boronat, MJ.; Herrero Villen, MA. (2015). Propiedades ácido-base de las sales. http://hdl.handle.net/10251/5137

Celdas galvánicas o voltaicas

Las reacciones redox son procesos donde la energía que se libera en una reacción espontánea puede transformase en energía eléctrica. El dispositivo donde tiene lugar este tipo de proceso recibe el nombre de pila o celda galvánica, y son un tipo de las llamadas celdas electroquímicas.Atienza Boronat, MJ.; Herrero Villen, MA.; Noguera Murray, PS.; Tortajada Genaro, LA.; Morais Ezquerro, SB. (2016). Celdas galvánicas o voltaicas. http://hdl.handle.net/10251/68314DE

¿Cómo evaluaremos la competencia transversal “Planificación y gestión del tiempo” a un alumno de primer curso?

[EN] Learning the competency "Planning and time management" is essential in the current context of university education. Especially interesting is its monitoring in novel freshman since their rates of failure and dropout are often related to mismanagement of available resources, including particularly time planning. The role of instructor is to select the best tools for teaching and assessing the competency, without affecting the course of a particular subject. However, the lack of prior experience in the university community generating uncertainly regarding implementation. In this paper, a study of the skills associated with this competency combined with a review of published procedures is presented. The context analysis was used to develop a set of guidelines and recommendations of interest to be included during the development of a first-year course. It will also be useful as a support for the acquisition and evaluation of these skills. The potential benefits of the teaching action are to enhance academic performance and, therefore, the satisfaction of students and teachers[ES] El aprendizaje de la competencia “Planificación y gestión del tiempo” es fundamental en el actual marco de la educación universitaria. Es especialmente interesante su evaluación en alumnos noveles dado sus tasas de fracaso y abandono, muchas veces asociadas con una gestión errónea de los recursos disponibles, entre ellos particularmente el tiempo. El papel del profesor es seleccionar las mejores herramientas para la enseñanza y evaluación de esta competencia, sin afectar el transcurso de la asignatura.Sin embargo, la falta de claras experiencias previas origina incertidumbres en la comunidad universitaria respecto su implementación. En esta comunicación, se presenta un estudio de las destrezas asociadas a esta competencia, combinado con una revisión de los procedimientos publicados. El análisis del contexto educativo ha permitido elaborar una serie de pautas y recomendaciones de interés para incorporar durante el desarrollo de una asignatura de primer curso. También servirá como material de apoyo para la adquisición y la evaluación de estas destrezas. Los beneficios potenciales de la acción docente son mejorar los rendimientos académicos y, por tanto, la satisfacción de los alumnos y los profesores.Tortajada Genaro, LA.; Herrero Villen, MA.; Noguera Murray, PS.; Morais Ezquerro, SB.; Atienza Boronat, MJ. (2015). ¿Cómo evaluaremos la competencia transversal “Planificación y gestión del tiempo” a un alumno de primer curso?. En In-Red 2015 - CONGRESO NACIONAL DE INNOVACIÓN EDUCATIVA Y DE DOCENCIA EN RED. Editorial Universitat Politècnica de València. https://doi.org/10.4995/INRED2015.2015.1533OC

Conceptos básicos de oxidación - reducción

Las reacciones de oxidación-reducción, también denominadas redox, son procesos de transferencia de electrones. La energía que se libera en una reacción espontánea puede transformarse en energía eléctrica. En un proceso redox se produce la oxidación de una especie a la vez que otra se reduceHerrero Villen, MA.; Atienza Boronat, MJ.; Morais Ezquerro, SB.; Tortajada Genaro, LA.; Noguera Murray, PS. (2016). Conceptos básicos de oxidación - reducción. http://hdl.handle.net/10251/68298DE

Disoluciones reguladoras de pH

El objeto de aprendizaje tiene como objetivo que ela lumno aprenda a identificar una disolución reguladora, explique cómo actúan las disoluciones reguladoras frente a pequeñas adiciones de ácidos o bases fuertes, y sepa calcular el pH de disoluciones reguladorasTortajada Genaro, LA.; Noguera Murray, PS.; Atienza Boronat, MJ.; Herrero Villen, MA.; Morais Ezquerro, SB. (2015). Disoluciones reguladoras de pH. http://hdl.handle.net/10251/5137

Pilas electrolíticas

En las celdas electrolíticas se produce una reacción química no espontánea cuando se aporta energía eléctrica. En este tipo de celda sucede el proceso contrario al de una celda galvánica.Atienza Boronat, MJ.; Herrero Villen, MA.; Morais Ezquerro, SB.; Noguera Murray, PS.; Tortajada Genaro, LA. (2016). Pilas electrolíticas. http://hdl.handle.net/10251/68342DE

Incertidumbre en las mediciones

En Química, como en cualquier ciencia experimental, cuando hablamos de incertidumbre de una medida se usan diferentes términos para describir la confianza de los resultados obtenidos y se establecen reglas para expresarlos correctamente. En este trabajo vamos a centrarnos en dos parámetros, exactitud y precisión, que se utilizan en el análisis de fiabilidad de los datos, así como en la presentación de los valores obtenidos, en concreto en el número de cifras significativas.Morais Ezquerro, SB.; Atienza Boronat, MJ.; Herrero Villen, MA.; Noguera Murray, PS.; Tortajada Genaro, LA. (2018). Incertidumbre en las mediciones. http://hdl.handle.net/10251/10435